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- 저자 : Vanessa C Rodrigues(Vanessa C Rodrigues ) (검색결과 4 건)
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Lara E Silva(Lara E Silva ),Vanessa C Rodrigues(Vanessa C Rodrigues ),Gisele R Vicente(Gisele R Vicente ),Marco Machado(Marco Machado ) 사피엔시아 2021 Exercise Medicine Vol.5 No.-
Objectives: Plyometrics has been used in sports training and many aspects of this modality are poorly understood. This study measures the magnitude of variations of serum creatine kinase (CK) activity and perceived muscle soreness after one session of Countermovement Jump (CMJ) session and compares 1 and 3 minutes rest intervals between each sets. Methods: A group of 30 young male was dividing in two groups and performed ten sets of ten CMJ with 1 or 3 minutes rest interval between sets. Before and after session (24, 48, and 72-h) they informed the muscle soreness perception and blood samples were collected for dosage of serum CK activity. Two (time) x Two (treatment) ANOVA was used to test differences in serum CK activity and muscle soreness. The alpha level was set at 0.05. Results: Serum CK activity measured 48 h after session augments ~2 and ~1.5 times in 1 min and 3 min rest interval respectively. No significant difference between groups that rests 1 or 3 minutes between sets was found. Muscle soreness rises 24 h after session without significant differences between groups. Conclusions: The findings show that the CMJ increases perceived muscle soreness and increases the serum CK activity, and the intervals of rest do not modulate these variables.
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Kinetic characterization of a novel acid ectophosphatase from Enterobacter asburiae
Vanessa Sayuri Sato,Renato F. Galdiano Júnior,Gisele Regina Rodrigues,Eliana G. M. Lemos,João Martins Pizauro Junior 한국미생물학회 2016 The journal of microbiology Vol.54 No.2
Expression of acid ectophosphatase by Enterobacter asburiae, isolated from Cattleya walkeriana (Orchidaceae) roots and identified by the 16S rRNA gene sequencing analysis, was strictly regulated by phosphorus ions, with its optimal activity being observed at an inorganic phosphate concentration of 7 mM. At the optimum pH 3.5, intact cells released p-nitrophenol at a rate of 350.76 ± 13.53 nmol of p-nitrophenolate (pNP)/min/108 cells. The membrane-bound enzyme was obtained by centrifugation at 100,000 × g for 1 h at 4°C. p-Nitrophenylphosphate (pNPP) hydrolysis by the enzyme follows “Michaelis-Menten” kinetics with V = 61.2 U/mg and K0.5 = 60 μM, while ATP hydrolysis showed V = 19.7 U/mg, K0.5 = 110 μM, and nH = 1.6 and pyrophosphate hydrolysis showed V = 29.7 U/mg, K0.5 = 84 μM, and nH = 2.3. Arsenate and phosphate were competitive inhibitors with Ki = 0.6 mM and Ki = 1.8 mM, respectively. p-Nitrophenyl phosphatase (pNPPase) activity was inhibited by vanadate, while p-hydroxymercuribenzoate, EDTA, calcium, copper, and cobalt had no inhibitory effects. Magnesium ions were stimulatory (K0.5 = 2.2 mM and nH = 0.5). Production of an acid ectophosphatase can be a mechanism for the solubilization of mineral phosphates by microorganisms such as Enterobacter asburiae that are versatile in the solubilization of insoluble minerals, which, in turn, increases the availability of nutrients for plants, particularly in soils that are poor in phosphorus.
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Ilda P. Ribeiro,Joana M. Rodrigues,Alexandra Mascarenhas,Vanessa Marques,Francisco Caramelo,Maria J. Julião,Thomas Liehr,Joana B. Melo,Isabel M. Carreira 한국유전학회 2019 Genes & Genomics Vol.41 No.10
Background Head and neck squamous cell carcinoma cell lines are useful preclinical models to understand the molecular processes underlying the development of such tumors, and to establish targeted therapies. Objective We performed a comprehensive (cyto)genomic and epigenetic characterization of three new established primary human head and neck squamous cell carcinoma cultures and an established, yet undercharacterized cell line: BICR 10. Methods Karyotyping, multiplex fluorescence in situ hybridization, array comparative genomic hybridization and methylation- specific multiplex ligation-dependent probe amplification were applied. Results The three primary cultures turned out to be a near-triploid and BICR 10 near-diploid. Banding and molecular cytogenetic analysis revealed non-random numerical and structural aberrations. The most common rearrangements identified in BICR 10 cell line were non-complex derivatives of reciprocal translocations, in which the breakpoints often appeared in centromeric/near-centromeric regions. In the 3 primary cell cultures the most common rearrangements observed were iso- and derivatives chromosomes derived from translocations. Overall, gains of 7p, 8q and losses at 3p, 8p, 9p, 18q and Xp were present in all four studied samples. Among the analyzed genes, BICR 10 cell line exhibited enhanced methylation of gene promoter; however, in all studied samples PAX5, WT1 and GATA5 were methylated. Conclusion The here reported comprehensive characterization of BICR 10 cell line and the new established cultures enriches the resources available for head and neck cancer research, especially for testing therapeutic agents.
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Katia Cristina Toyokawa-Sperandio,Ana Clá,udia de Castro Ferreira Conti,Thais Maria Freire Fernandes,Renata Rodrigues de Almeida-Pedrin,Marcio Rodrigues de Almeida,Paula Vanessa Pedron Oltramari 대한치과교정학회 2021 대한치과교정학회지 Vol.51 No.5
Objective: To compare the magnitude of external apical root resorption (EARR) 6 months after starting orthodontic treatment using orthodontic aligners (OAs) and fixed appliances (FAs). Methods: This parallel randomized clinical trial included 40 patients randomized into two groups: OA group (n = 20, 160 incisors) and FA group (n = 20, 160 incisors). For evaluation of the tooth length, periapical radiographs and standardized linear measurements of the maxillary and mandibular incisors were acquired before (T0) and 6 months after treatment initiation (T1). EARR was calculated through the difference in length between the two time points (T1–T0). Statistical comparisons were performed by means of using t -tests, chi-squared test and covariance analysis (a = 5%). Results: Rounding of the root apex was observed in both groups; the resorption involved 2.88% of the root length, so 97.12% of the tooth length remained intact. Intragroup comparisons between the two time points revealed a significant difference, with (T1–T0) ranging from −0.52 to −0.88 mm in the FA group and from −0.52 to −0.85 mm in the OA group. In the intergroup comparisons, only tooth #21 presented a statistically significant difference (OA: −0.52 ± 0.57 mm, FA: −0.86 ± 0.60 mm); however, the overall differences between groups were not clinically relevant, ranging from 0.03 to 0.35 mm. Conclusions: OA and FA treatment resulted in a similar degree of EARR in the maxillary and mandibular incisors at 6 months after treatment initiation. However, the amount of resorption was small and does not impair tooth longevity.
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