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      • Inhomogeneous Ferromagnetism and Spin-Glass-Like Behavior in <inline-formula> <tex-math notation='TeX'> <tex> $({\rm Nd}_{1-x}{\rm Y}_{x})_{0.7}{\rm Sr}_{0.3}{\rm MnO}_{3}$ </tex> </tex-math></inline-formula> With <inline-formula> <tex-math notation='TeX

        Phan, T. L.,Nguyen, V. D.,Ho, T. A.,Khiem, N. V.,Thanh, T. D.,Phuc, N. X.,Thang, P. D.,Yu, S. C. IEEE 2014 IEEE transactions on magnetics Vol.50 No.6

        <P>The magnetic properties of polycrystalline ceramic samples (Nd1-xYx)(0.7)Sr0.3MnO3 with x = 0.21 - 0.35 were studied by means of dc magnetization and ac susceptibility measurements. Experimental results reveal a strong decrease of the ferromagnetic (FM)-paramagnetic phase-transition temperature (T-C) from 97 to 65 K as increasing x from 0.21 to 0.35, respectively. There is magnetic inhomogeneity associated with short-range FM order. Particularly, the samples undergo a spin-glass (SG) phase transition at the so-called blocking temperature (T-B) below T-C, which shifts toward lower temperatures with increasing the applied field, Hex; T-B -> T-g (the SG phase-transition temperature) as H-ex -> 0. The existence of the SG behavior in these samples was also confirmed by frequency (f) dependences of the ac susceptibility. For the in-phase/real component, chi' (T), it shows a frequency-dependent peak at the SG freezing temperature (T-f); T-f -> T-g as f -> 0. Dynamics of this process were analyzed by means of the slowing down scaling law, tau/tau(0) proportional to (T-f/T-g - 1)(-zv), where tau(0) and zv are the characteristic time and critical exponent, respectively. Fitting the experimental T-f(f) data to the scaling law gave the results of zv = 10.1-12.3 and tau(0) = 10(-21)-10(-15)s. These values are different from those expected for canonical SG systems with zv = 10 and tau(0) = 10(-13)s, revealing the cluster-SG behavior of (Nd1-xYx)(0.7)Sr0.3MnO3 samples. Notably, the increase in Y content leads to the shift of tau(0) and zv values toward those of canonical SG systems, which is ascribed to an expansion of SG clusters.</P>

      • Mathematical models applied to dispersal of pest populations in greenhouse

        T. V. Nguyen,C.Xia,B.K.Chung,H.Y.Kim,T.S.Chona 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

        A genome project has been launched and aims to sequence total genome of Cotesia plutellae bracovirus. On this process, several open reading frames (ORFs) have been identified. This study was intended to clone and express protein tyrosine phosphatase genes, PTP1 and PTP6. The ORFs of these two genes consist of 900 and 891 bp, respectively. PTP1 and PTP6 are genes of a group of genes that has been implicated as important regulatory components in cell growth, differentiation and malignant transformation by certain viruses. In this work,we studied the cloning and expression patterns of these genes in Plutella xylostella, a lepidopteran host of C. plutellae. A polymerase chain reaction (PCR) produced the corresponding products of PTP1/6. These PCR products were cloned and expressed using an expression vector pBAD-TOPO, and then over-expressed using an inducer, L-arabinose. Then the purified proteins were confirmed using Western blotting (immunoblotting using V5 antibody) and the apparent molecular weights of both proteins were about 40 kDa. Expression of PTP genes were analyzed in the parasitized P. xylostella by realtime RT-PCR, which indicated late expression pattern of both PTPs during parasitization. We are pursuing to elucidate their physiological function in the parasitized host insect.

      • KCI등재

        The Influence of Mixed Solvents Volatility on Charge State Distribution of Peptides During Positive Electrospray Ionization Mass Spectrometry

        ( Birthe V. Nielsen ),( Daniel A. Abaye ),( Minh T. L. Nguyen ) 한국질량분석학회 2017 Mass spectrometry letters Vol.8 No.2

        Understanding the mechanisms that control and concentrate the observed electrospray ionisation (ESI) response from peptides is important. Controlling these mechanisms can improve signal-to-noise ratio in the mass spectrum, and enhances the generation of intact ions, and thus, improves the detection of peptides when analysing mixtures. The effects of different mixtures of aqueous: organic solvents (25, 50, 75%; v/v): formic acid solution (at pH 3.26) compositions on the ESI response and charge-state distribution (CSD) during mass spectrometry (MS) were determined in a group of biologically active peptides (molecular wt range 1.3 - 3.3 kDa). The ESI response is dependent on type of organic solvent in the mobile phase mixture and therefore, sol-vent choice affects optimal ion intensities. As expected, intact peptide ions gave a more intense ESI signal in polar protic solvent mixtures than in the low polarity solvent. However, for four out of the five analysed peptides, neither the ESI response nor the CSD were affected by the volatility of the solvent mixture. Therefore, in solvent mixtures, as the composition changes during the evaporation processes, the pKb of the amino acid composition is a better predictor of multiple charging of the peptides.

      • KCI등재

        Discordance between quantitative ultrasound and dual-energy X-ray absorptiometry in bone mineral density: The Vietnam Osteoporosis Study

        Huy G. Nguyen,Khanh B. Lieu,Thao P. Ho-Le,Lan T. Ho-Pham,Tuan V. Nguyen 대한골다공증학회 2021 Osteoporosis and Sarcopenia Vol.7 No.1

        Objectives: Calcaneal quantitative ultrasound measurement (QUS) has been considered an alternative to dual-energy X-ray absorptiometry (DXA) based bone mineral density (BMD) for assessing bone health. This study sought to examine the utility of QUS as an osteoporosis screening tool by evaluating the correlation between QUS and DXA. Methods: The study was a part of the Vietnam Osteoporosis Study that involved 1270 women and 773 men aged 18 years and older. BMD at the femoral neck, total hip and lumbar spine was measured using DXA. Osteoporosis was diagnosed based on the femoral neck T-score using World Health Organization criteria. Broadband ultrasound attenuation (BUA) at the calcaneus was measured by QUS. The concordance between BUA and BMD was analyzed by the linear regression model. Results: In all individuals, BUA modestly correlated with femoral neck BMD (r ¼ 0.35; P < 0.0001) and lumbar spine BMD (r ¼ 0.34; P < 0.0001) in both men and women. In individuals aged 50 years and older, approximately 16% (n ¼ 92/575) of women and 3.2% (n ¼ 10/314) of men were diagnosed to have osteoporosis. Only 0.9% (n ¼ 5/575) women and 1.0% (n ¼ 3/314) men were classified as “Low BUA”. The kappa coefficient of concordance between BMD and BUA classification was 0.09 (95% CI, 0.04 to 0.15) for women and 0.12 (95% CI, 0.03 to 0.22) for men. Conclusions: In this population-based study, QUS BUA modestly correlated with DXA BMD, suggesting that BUA is not a reliable method for screening of osteoporosis

      • SCOPUSKCI등재

        The Influence of Mixed Solvents Volatility on Charge State Distribution of Peptides During Positive Electrospray Ionization Mass Spectrometry

        Nielsen, Birthe V.,Abaye, Daniel A.,Nguyen, Minh T.L. Korean Society for Mass Spectrometry 2017 Mass spectrometry letters Vol.2 No.4

        Understanding the mechanisms that control and concentrate the observed electrospray ionisation (ESI) response from peptides is important. Controlling these mechanisms can improve signal-to-noise ratio in the mass spectrum, and enhances the generation of intact ions, and thus, improves the detection of peptides when analysing mixtures. The effects of different mixtures of aqueous: organic solvents (25, 50, 75%; v/v): formic acid solution (at pH 3.26) compositions on the ESI response and charge-state distribution (CSD) during mass spectrometry (MS) were determined in a group of biologically active peptides (molecular wt range 1.3 - 3.3 kDa). The ESI response is dependent on type of organic solvent in the mobile phase mixture and therefore, solvent choice affects optimal ion intensities. As expected, intact peptide ions gave a more intense ESI signal in polar protic solvent mixtures than in the low polarity solvent. However, for four out of the five analysed peptides, neither the ESI response nor the CSD were affected by the volatility of the solvent mixture. Therefore, in solvent mixtures, as the composition changes during the evaporation processes, the $pK_b$ of the amino acid composition is a better predictor of multiple charging of the peptides.

      • SCISCIESCOPUS

        Direct typing and molecular evolutionary analysis of field samples of foot-and-mouth disease virus collected in Viet Nam between 2006 and 2007

        Lee, K.N.,Nguyen, T.,Kim, S.M.,Park, J.H.,Do, H.T.,Ngo, H.T.,Mai, D.T.,Lee, S.Y.,Nguyen, C.V.,Yoon, S.H.,Kweon, C.H.,Cho, I.S.,Kim, H. Elsevier Scientific Pub. Co 2011 Veterinary microbiology Vol.147 No.3

        In this study, we used universal or duplex serotype-specific (O and Asia 1) RT-PCR to analyze clinical field samples of foot-and-mouth disease virus (FMDV) or virus isolates collected in Viet Nam between 2006 and 2007. We found viral serotypes O and Asia 1 circulating concurrently during this period. Direct sequencing of type-specific RT-PCR products revealed the existence of three different topotypes of serotype O: Southeast Asia (SEA), Middle East-South Asia (ME-SA), and Cathay. Of these, SEA was most prevalent during the period. All samples of serotype Asia 1 belonged to genetic group V. Based on the rooted maximum likelihood phylogenetic trees inferred from the VP1 region, new lineages in topotype SEA were originating from Viet Nam, and group V strains of Asia 1 have undergone fewer passages from the common ancestor, compared with other genetic groups. The co-circulation of different types of FMDV may complicate the individual or population genomic structures of FMDV and make conventional multiplex diagnostic methods and phylogenetic analyses with relevant evolutionary models essential in Viet Nam.

      • KCI등재후보

        The Vietnam Osteoporosis Study: Rationale and design

        Lan T. Ho-Pham,Tuan V. Nguyen 대한골다공증학회 2017 Osteoporosis and Sarcopenia Vol.3 No.2

        Objectives: Osteoporosis and fracture impose a significant health care burden on the contemporary populations in developing countries. The Vietnam Osteoporosis Study (VOS) sought to assess the burden of osteoporosis and its comorbidities in men and women. Methods: The study was designed as a population-based family investigation in which families were randomly recruited from Ho Chi Minh City, Vietnam. Individuals were assessed for bone health, including bone mineral density (BMD) and body composition and trabecular and cortical bone properties by pQCT (peripheral quantitative computed tomography). Fasting blood samples were obtained for the analysis of plasma glucose, glycosylated hemoglobin, and bone turnover markers. Genomic DNA extraction from whole blood samples for further genetic and genomic analyses. Results: We have recruited more than 4157 individuals from 817 families. The average age of participants was 51, with approximately 45% of the individuals aged 50 years and older. Approximately 3% of participants were obese (body mass index ? 30 kg/m2), and 21% were overweight. Notably, 11% of participants aged 40 years and older were diabetic. Among those aged 50 years and older, approximately 14% of women and 5% of men had osteoporosis (i.e., femoral neck BMD T-scores ? ?2.5). There were modest correlations between volumetric BMD and areal BMD. Conclusions: VOS is a major bone research project in Vietnam aimed at comprehensively documenting the burden osteoporosis, its co-occurrence of chronic diseases, and their underlying etiologies. The Study will make important contributions to the literature of bone health worldwide.

      • Liquid-liquid extraction of Cd(II) from pure and Ni/Cd acidic chloride media using Cyanex 921: A selective treatment of hazardous leachate of spent Ni-Cd batteries

        Choi, S.Y.,Nguyen, V.T.,Lee, J.c.,Kang, H.,Pandey, B.D. Elsevier Scientific Pub. Co 2014 Journal of hazardous materials Vol.278 No.-

        The present paper is focused on solvent extraction of hazardous Cd(II) from acidic chloride media by Cyanex 921, a new extractant mixed with 10% (v/v) TBP in xylene. The optimum conditions for extraction and stripping of Cd(II) were investigated with an aqueous feed of 0.1mol/L Cd(II) in 2.0mol/L HCl. McCabe-Thiele diagram was in good agreement with the simulation studies, showing the quantitative extraction (99.9%) of Cd(II) within two counter-current stages utilizing 0.30mol/L Cyanex 921 at O/A ratio of 3/2 in 10min. Stoichiometry of the complexes extracted was determined and confirmed by numerical treatment and graphical method, revealing the formation of HCdCl<SUB>3</SUB>.2L and HCdCl<SUB>3</SUB>.4L for Cyanex 921(L) concentration in the range 0.03-0.1mol/L and 0.1-1.0mol/L, respectively. The thermodynamic parameters for the extraction of cadmium were also determined. The stripping efficiency of cadmium from the loaded organic with 0.10mol/L HCl was 99.6% in a three-stage counter-current process at an O/A ratio of 2/3. Cyanex 921 was successfully applied for the separation of Cd(II) from Ni(II) in the simulated leach liquor of spent Ni-Cd batteries. The study demonstrates the applicability of the present hydrometallurgical approach for the treatment of hazardous waste, the spent Ni-Cd batteries.

      • The evolutionary dynamics of highly pathogenic avian influenza H5N1 in south-central Vietnam reveals multiple clades evolving from Chinese and Cambodian viruses

        Nguyen, T.H.,Than, V.T.,Thanh, H.D.,Nguyen, V.Q.,Nguyen, K.H.,Nguyen, D.T.,Park, J.H.,Chung, I.S.,Jeong, D.G.,Chang, K.T.,Oh, T.K.,Kim, W. Pergamon Press 2015 Comparative immunology, microbiology and infectiou Vol.42 No.-

        In Vietnam, highly pathogenic avian influenza (HPAI), such as that caused by H5N1 viruses, is the most highly contagious infectious disease that has been affecting domestic poultry in recent years. Vietnam might be an evolutionary hotspot and a potential source of globally pandemic strains. However, few studies have reported viruses circulating in the south-central region of Vietnam. In the present study, 47 H5N1-positive samples were collected from both vaccinated and unvaccinated poultry farms in the South Central Coast region of Vietnam during 2013-2014, and their genetic diversity was analyzed. A common sequence motif for HPAI virus was identified at HA-cleavage sites in all samples: either RERRRKR/G (clades 2.3.2.1c and 2.3.2.1a) or REGRRKKR/G (clade 1.1.2). Phylogenetic analysis of HA genes identified three clades of HPAI H5N1: 1.1.2 (n=1), 2.3.2.1a (n=1), and 2.3.2.1c (n=45). The phylogenetic analysis indicated that these Vietnamese clades may have evolved from Chinese and Cambodian virus clades isolated in 2012-2013 but are less closely related to the clades detected from the Tyva Republic, Bulgaria, Mongolia, Japan, and Korea in 2009-2011. Detection of the coexistence of virus clades 2.3.2.1 and the very virulent 1.1.2 in the south-central regions suggests their local importance and highlights concerns regarding their spread, both northwards and southwards, as well as the potential for reassortment. The obtained data highlight the importance of regular identification of viral evolution and the development and use of region-specific vaccines.

      • KCI등재

        Sorting CD4+ T Cells in Blood by Using Magnetic Nanoparticles Coated with Anti-CD4 Antibody

        N. T. Khuat,V. T. A. Nguyen,T. N. Phan,L. H. Hoang,C. V. Thach,N. H. Hai,N. Chau 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.6

        We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then, the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4- MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes. We used Fe3O4 magnetic nanoparticles (MNPs) which are coated with antiCD4 monoclonal antibody to bind selectively onto membranes of CD4+ T cells (hereafter antiCD4-MNPs). The antiCD4-MNPs were prepared through direct covalent interaction between the carboxyl group of the antiCD4 antibody and the amino group of amino-modified MNPs. The antiCD4-MNPs were mixed with human blood cells, followed by bursting the red blood cells with hypotonic buffer; then, the antiCD4-MNPs coated cells were separated by using a magnet. We observed the number of cells bound with magnetite clusters and particles. When fluorescence isothiocyanate labeled antiCD4- MNPs was used to observe the CD4+ T cells, the fluorescent intensity was improved by about two times compared to that when cells were labeled with the antiCD4 antibody only. This is a potential method to sort helper CD4+ T cells for observation under conventional microscopes.

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