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( Ujjal Kumar Nath ),( Hoy Taek Kim ),( Khadiza Khatun ),( Jong In Park ),( Kwon Kyoo Kang ),( Ill Sup Nou ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.2
Rapeseed is a member of family Brassicaceae, cultivated as oil crop. Rapeseed oil is being utilized from early civilization, but its popularity being declined from the mid-nineteenth century due to presence of erucic acid (C22:1) and glucosinolates. Thereby, several attempts have been made to develop cultivars free from those toxins. In the past 20 years, breeders got success in developing ``00``- quality rapeseed, known as ``Canola``. The target mutagenesis of fae-1 and fae-2 of Brassica napus ensured such success. Thereafter, ``canola`` regains its market as a healthy vegetable oil. Moreover, high oleic acid rapeseed lines, with 86% oleic acid, have been developed by using chemical mutagenesis of FAD2 alleles responsible for desaturation of oleic acid (C18:1) to linoleic acid (C18:2). Recently, high erucic acid rapeseed oil regained interest for biodegradable plastic, cosmetic, emollient industries and for biodiesel. Therefore, breeding approaches have been pursued; unfortunately, that were failed to reach erucic acid level beyond 50% in seed-oil. Rapeseed genotypes over-expressed with Ld-LPAAT separately and Ld-LPAAT-FAE chimaric construct together were tried but failed to reach the erucic acid content more than 60%. Thereof, combined effort of conventional breeding and transgenic approaches are brought together to overcome three hypothesized bottlenecks; reviewed in this article, which restricted erucic acid level near to 60%. Finally, rapeseed genotypes with 78% erucic acid were developed successfully. This material is now available in Germany for using in emollient industries and for biodiesel. Therefore, this article is reviewed on the current status and future outlook for modification of fatty acid profiles of rapeseed oil for its end-use as food, industrial feed-stock and biodiesel.
Biological green synthesis of gold and silver nanoparticles
Sur, Ujjal Kumar Techno-Press 2014 Advances in nano research Vol.2 No.3
Nanomaterials synthesized by natural bioresources such as microorganisms, animals and plants in nature can also be synthesized in laboratories even on large scale. This is considered as an attractive prospect for eco-friendly or so-called green synthesis. Development of eco-friendly synthesis of biocompatible nanoparticles and their potential biomedical applications introduces the concept of nanobiotechnology. The lower cost and lesser side effects as compare to chemical methods of synthesis are the main advantages of biosynthesis. This review article demonstrates the role of various biological resources e.g. bacteria, fungi, actinomycetes, plant leaves, fruits and honey as well as animal tissues for the synthesis of nanoparticles mainly gold and silver with an overview of their potential applications.
Banerjee, Ujjal,Choudhuri, D.K. 한국곤충학회 1986 Korean journal of entomology Vol.16 No.1
Information about the toxic effects of nicotine on the biochemical components of the central nervous system is limited. A single sublethal dose of nicotine (54 $\mu\textrm{g}$/g body weight) was found to interfere with the enzyme acetylcholinesterase, acid phosphatase and nucleotidase. Acetylcho line is the familiar neurotransmitter in insects. But adenosine which acts as a putative neuro-transmitter during emergency period when acetylcholinesterase activity gets inhibited and transmission of nerve impulse becomes blocked is yet to be properly understood. The toxic stress produced due to nicotine treatment reduced the acetylcholinesterase activity and boosted up the activity of 5'-nucleotidase. The physiological significance of these findings have been discussed elaborately in this communication.
Haldar, Ujjal,Lee, Hyung-il American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.14
<P>Developing a simple and cheap analytical method for the selective detection and quantitative separation of toxic ions present in aqueous media is the biggest challenge faced by the chemosensing research community. Here, a 5,5-difluoro-1,3,7,9-tetramethyl-10-phenyl-5<I>H</I>-dipyrrolo-diazaborinine-derived water-soluble polymer integrated with thiosemicarbazone units was rationally designed and synthesized for the simultaneous detection and separation of Hg(II) ions in pure aqueous solution. The water-soluble polymer scaffold poly(<I>N</I>,<I>N</I>′-dimethyl acrylamide-<I>co</I>-5,5-difluoro-1,3,7,9-tetramethyl-10-phenyl-5<I>H</I>-dipyrrolo-diazaborinine-2-carbaldehyde) was synthesized by reversible addition-fragmentation chain transfer polymerization, followed by post-polymerization modification with thiosemicarbazide, leading to the formation of the target probe, P1. The nonemitting P1 exhibited bright yellow emission upon exposure to Hg(II) ions, with a limit of detection as low as 0.37 μM. This turn-on emission behavior triggered by Hg(II) ions might originate from the suppression of isomerization around the C═N bond of the thiosemicarbazone moiety caused by the formation of a coordination complex between P1 and Hg(II) ions. In addition, P1 displayed excellent selectivity toward Hg(II) ions over other metal cations. Finally, the selective removal of Hg(II) ions from an aqueous solution containing various metal ions was achieved by precipitation, which is probably caused by the fact that coordination complexes whereby Hg(II) ions acted as bridgeheads between P1 molecules had formed.</P> [FIG OMISSION]</BR>
Haldar, Ujjal,Lee, Hyung-il The Royal Society of Chemistry 2018 Polymer chemistry Vol.9 No.39
<P>The development of water-soluble polymeric probes capable of recognizing more than one analyte is quite challenging and has attracted significant attention. A water-soluble random copolymer (P1) consisting of hydrophilic <I>N</I>,<I>N</I>′-dimethyl acrylamide (DMA) and aldehyde-terminated difluoroboron dipyrromethene (BODIPY) methacrylate segment was synthesized <I>via</I> the reversible addition-fragmentation chain transfer (RAFT) polymerization for the detection of Hg<SUP>2+</SUP> and HSO4<SUP>−</SUP> ions, respectively. The aldehyde terminal end of P1 was post-functionalized with 4-amino phenol through a Schiff-base condensation reaction, leading to a final polymeric probe (P2). P2 exhibited both colorimetric and fluorimetric responses according to the pH of the aqueous solution or bubbling the solution with CO2, manifesting itself as a pH indicator and CO2 sensor. Upon the addition of Hg<SUP>2+</SUP> ions, P2 showed apparent color changes from pink to light yellow under ambient light and colorless to bright green emission under 365 nm UV light with a very low limit of detection (LOD = 1.10 μM). At low pH, no change in the fluorescence enhancement was observed upon exposure to Hg<SUP>2+</SUP> ions, whereas the fluorescence intensity of P2 increased remarkably at high pH. Similar sensing behavior was observed for HSO4<SUP>−</SUP> ions with a LOD of 1.12 μM. P2 was quite selective toward Hg<SUP>2+</SUP> and HSO4<SUP>−</SUP> over the other metal cations and anions tested, respectively. The detection of both Hg<SUP>2+</SUP> and HSO4<SUP>−</SUP> ions originated from the direct hydrolysis of imine bonds of P2.</P>
Sur, Ujjal Kumar Techno-Press 2013 Advances in nano research Vol.1 No.2
Surface-enhanced Raman scattering (SERS) effect deals with the enhancement of the Raman scattering intensity by molecules in the presence of a nanostructured metallic surface. The first observation of surface-enhanced Raman spectra was in 1974, when Fleischmann and his group at the University of Southampton, reported the first high-quality Raman spectra of monolayer-adsorbed pyridine on an electrochemically roughened Ag electrode surface. Over the last thirty years, it has developed into a versatile spectroscopic and analytical technique due to the rapid and explosive progress of nanoscience and nanotechnology. This review article describes the recent development in field of surface-enhanced Raman scattering research, especially fabrication of various SERS active substrates, mechanism of SERS effect and its various applications in both surface sciences and analytical sciences.
Swati Dasgupta,Ujjal K Ray,Arpita Ghosh Mitra,Deboshree M. Bhattacharyya,Ashis Mukhopadhyay,Priyabrata Das,Sudeshna Gangopadhyay,Sudip Roy,Soma Mukhopadhyay 대한혈액학회 2017 Blood Research Vol.51 No.2
Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.