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Polygalacturonase (PG) Gene Expression in Musa acuminata Cultivars from Kerala
Thulasy Gayathri,Ashalatha Sunkarankutty Nair,Vidhu Aniruddha Sane 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.6
Banana cannot be preserved for a long timeafter harvesting due to a short shelf life. Fruit softening isassociated with textural changes due to disassembly of theprimary cell wall and modification of the structure andcomposition of various polysaccharides. Cell wall degradationis caused by the action of various cell wall hydrolaseenzymes. Polygalacturonase (PG) is the key enzymeinvolved in this process. The ripening period is different incultivars maintained under domesticated cultivation inKerala. PG activity was profiled in eight Musa acuminatacultivars from Kerala and expression analysis of the PGgene was accomplished using semi-quantitative RT-PCR. Maximum PG activity was observed in Palayankodan andminimum activity was observed in Kadali. Gene expressionanalysis showed variation between ethylene treated fruitsand controls in Palayankodan, whereas the expressionpatterns in Kadali were similar. The fruit softening processis cultivar specific.