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RISS 인기검색어
- 검색키워드
- 저자 : Tengku Azman Tengku Mohd (검색결과 2 건)
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Effects of Rapamycin on Cell Apoptosis in MCF-7 Human Breast Cancer Cells
Tengku Din, Tengku Ahmad Damitri Al-Astani,Seeni, Azman,Khairi, Wirdatul-Nur Mohd,Shamsuddin, Shaharum,Jaafar, Hasnan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24
Background: Rapamycin is an effective anti-angiogenic drug. However, the mode of its action remains unclear. Therefore, in this study, we aimed to elucidate the antitumor mechanism of rapamycin, hypothetically via apoptotic promotion, using MCF-7 breast cancer cells. Materials and Methods: MCF-7 cells were plated at a density of $1{\times}10^5$ cells/well in 6-well plates. After 24h, cells were treated with a series of concentrations of rapamycin while only adding DMEM medium with PEG for the control regiment and grown at $37^{\circ}C$, 5% $CO_2$ and 95% air for 72h. Trypan blue was used to determine the cell viability and proliferation. Untreated and rapamycin-treated MCF-7 cells were also examined for morphological changes with an inverted-phase contrast microscope. Alteration in cell morphology was ascertained, along with a stage in the cell cycle and proliferation. In addition, cytotoxicity testing was performed using normal mouse breast mammary pads. Results: Our results clearly showed that rapamycin exhibited inhibitory activity on MCF-7 cell lines. The $IC_{50}$ value of rapamycin on the MCF-7 cells was determined as $0.4{\mu}g/ml$ (p<0.05). Direct observation by inverted microscopy demonstrated that the MCF-7 cells treated with rapamycin showed characteristic features of apoptosis including cell shrinkage, vascularization and autophagy. Cells underwent early apoptosis up to 24% after 72h. Analysis of the cell cycle showed an increase in the G0G1 phase cell population and a corresponding decrease in the S and G2M phase populations, from 81.5% to 91.3% and 17.3% to 7.9%, respectively. Conclusions: This study demonstrated that rapamycin may potentially act as an anti-cancer agent via the inhibition of growth with some morphological changes of the MCF-7 cancer cells, arrest cell cycle progression at G0/G1 phase and induction of apoptosis in late stage of apoptosis. Further studies are needed to further characterize the mode of action of rapamycin as an anti-cancer agent.
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Chemical composition of rainwater harvested in East Malaysia
Siti Nor Fazillah Abdullah,Azimah Ismail,Hafizan Juahir,R Badlishah Ahmad,Fathurrahman Lananan,Noor Muzlinda Hashim,Nadiana Ariffin,Munirah Abdul Zali,Tengku Azman Tengku Mohd,Md Hafriz Fikrie Hussin 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.2
As part of the implementation of a rainwater harvesting system as an alternative water source supply for non-potable use, therefore the characteristic of chemical compounds was significantly explored. The Department of Chemistry, Malaysia, gave the data set for three years (2017-2019). Some chemometric techniques, including PCA, were performed to identify the dimensionality of the rainwater data, hence establishing the rainfall index"s purity to determine the quality of rainwater in the study area. Discriminant analysis managed to differentiate each rain gauge station. Cluster analysis was then applied to perform smaller group of rain gauge stations. The result demonstrates that sea salt, secondary aerosols, trace metals, crustal origin, and organic acid dominated the dimensionality of rainwater data with a total variance of 53.38% and indicated that the PRI was significantly diversified into good purity of rainfall index (GPRI), (Labuan and Danum Valley), moderate purity of rainfall index (MPRI), (Kuching and Tawau) and bad purity of rainfall index (BPRI), (Kota Kinabalu and Bintulu). From the study, it can be stipulated that the chemical composition of rainwater in the study area was attributable to the local activities.
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