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RISS 인기검색어
- 검색키워드
- 저자 : Tayyab Husnain (검색결과 3 건)
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Farzana, Khanum,Husnain, Tayyab,Riazuddin, Sheikh 한국식물학회 1998 Journal of Plant Biology Vol.41 No.2
An efficient protocol for in vitro micropropagation of seven indica rice varieties was developed from meristem culture. Meristem (leaf base) was isolated from different age of seedlings and cultured on MS medium without hormones and supplemented with different concentrations of NAA and BAP. Regeneration of plantlets from meristem was observed within five days of culture. The meristem isolated from 4-day old seedlings gave highest regeneration on hormone free MS medium. Histological study of meristem (leaf base) from 4-day old seedlings confirmed the presence of meristematic cells. Regenerated plants were multiplied on MS medium supplemented with 0.05 ㎎/L NAA and 5 ㎎/L BAP. An average of five plants were obtained from single regenerated meristem. The plants regenerated from meristem showed morphological uniformity.
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Transformation and Inheritance of Bt Genes in Gossypium hirsutum
( Bushra Rashid ),( Zafar Saleem ),( Tayyab Husnain ),( Sheikh Riazuddin ) 한국식물학회 2008 Journal of Plant Biology Vol.51 No.4
Transgenic plants offer many unique opportunities for managing pest populations. However, the inheritance, integration, and expression of multiple transgenes are prerequisite for maintaining sustainable resistance against insects in crops. We took a gene-pyramiding approach to produce Bt cotton expressing two Bt genes, cry1Ac and cry2A. Using sonication-assisted Agrobacterium-mediated transformation (SAAT), we achieved an efficiency of 6.26%. Putative transgenic plants were confirmed via PCR, Southern hybridization, and western-blotting. Those showing mortality of 75 to 100% for the second instar of Heliothis armigera (compared with 0% for the control) were considered Bt-positive. Transgenes were segregated according to a 3:1 Mendelian inheritance pattern in the T1 generation for Heliothis resistance. In our insect bioassay, the control plants showed >95% leaf damage, and insects reached the 4th instar stage of larval growth. In contrast, leaf damage on transgenic plants was limited to only a few bites, and insect mortality was 75 to 100%. ELISA confirmed transgene expression, and Bt protein was detected in leaf tissue. This performance was consistent with that of the parent transgenics. PCR and Southern blots verified integration of the cry1Ac and cry2A genes into the progeny. Therefore, this strategy provides a pathway toward cotton improvement and the development of durable resistance against insect damage.
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Field Evaluation and Fiber Analysis of Transgenic Cotton
Bushra Rashid,Ghazanfar Ali Khan,Tayyab Husnain,Sheikh Riazuddin 한국작물학회 2009 Journal of crop science and biotechnology Vol.12 No.3
We report the field evaluation of second generation of transgenic cotton expressing Bacillus thuringiensis (Bt) genes cry1Ac and cry2A under CaMV 35S promoter. Sixty-five transgenic lines were grown under RCBD design. Transgenic plants exhibited inherent ability to resist target insect (p < 0.05 and 0.01). Morphological studies showed significant reduction in plant height making them favorable for breeding. Yield was significantly increased for the transgenic lines. Fiber analysis showed improved gin turn out 40% for transgenic lines in comparison to 32% for non-transformed lines. Fibre quality of transgenic lines was not affected when compared with non transgenic lines. Inheritance pattern for transgenic lines suggests the need of further studies to understand the complex molecular mechanisms for resistance management and biosafety studies to develop new Bt cotton varieties. We report the field evaluation of second generation of transgenic cotton expressing Bacillus thuringiensis (Bt) genes cry1Ac and cry2A under CaMV 35S promoter. Sixty-five transgenic lines were grown under RCBD design. Transgenic plants exhibited inherent ability to resist target insect (p < 0.05 and 0.01). Morphological studies showed significant reduction in plant height making them favorable for breeding. Yield was significantly increased for the transgenic lines. Fiber analysis showed improved gin turn out 40% for transgenic lines in comparison to 32% for non-transformed lines. Fibre quality of transgenic lines was not affected when compared with non transgenic lines. Inheritance pattern for transgenic lines suggests the need of further studies to understand the complex molecular mechanisms for resistance management and biosafety studies to develop new Bt cotton varieties.
ScienceON
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