FEASIBILITY OF USING REMOTE SENSING AS A TOOL FOR SCHEDULING AND CONTROL OF DREDGING

GERRITSEN HERMAN,TATMAN SHARON,DIRKS WOUTER,HESSELMANS GERARD,NOORBERGEN HEIN 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

An Outbreak of Tularemia in Western Black Sea Region of Turkey

Saban Gurcan,Muserref Tatman-Otkun,Metin Otkun,Osman Kursat Arikan,Burcin Ozer 연세대학교의과대학 2004 Yonsei medical journal Vol.45 No.1

The aim of this study was to investigate the source and the size of a tularemia outbreak in a village located in a non-endemic area. Five patients from the same village were admitted to hospital with the same complaints all within one week of September 2001. Tularemia was suspected and a diagnosis was made after physical and anamnesis examinations. The village was visited the same week that the patients were admitted to the hospital, in the January and April 2002. The villagers were examined and screened serologically by microagglutination method and the water sources were investigated bacteriologically. A total of 14 people were found to be infected from the outbreak and the oropharyngeal form was the only clinical presentation. Antibody titers ranged between 1:80 and 1:640. The patients responded well to the aminoglycoside plus tetracycline therapy. Examination of the pipewater and three springs revealed that all the water sources were contaminated by coliforms, however, Francisella tularensis could not be isolated in glucose-cystine medium. Antibody levels stayed stable or decreased seven months after. Tularemia had not been reported in this area before, so the first patients were misdiagnosed. In conclusion tularemia should be considered in differential diagnosis of patients with fever, sore throat and cervical lymphadenopaties.

Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions

Kim, Sun Jeong,Tatman, Philip D,Song, Da-Hyun,Gee, Albert O,Kim, Deok-Ho,Kim, Sang Jun Dove Medical Press 2018 International journal of nanomedicine Vol.13 No.-

<P><B>Background</B></P><P>Tendon-derived stem cells (TDSCs) are key factors associated with regeneration and healing in tendinopathy. The aim of this study was to investigate the effects of mechanical stiffness and topographic signals on the differentiation of TDSCs depending on age and pathological conditions.</P><P><B>Materials and methods</B></P><P>We compared TDSCs extracted from normal tendon tissues with TDSCs from tendinopathic Achilles tendon tissues of Sprague Dawley rats in vitro and TDSCs cultured on nanotopographic cues and substrate stiffness to determine how to control the TDSCs. The tendinopathy model was created using a chemical induction method, and the tendon injury model was created via an injury-and-overuse method. Norland Optical Adhesive 86 (NOA86) substrate with 2.48 GPa stiffness with and without 800 nm-wide nanogrooves and a polyurethane substrate with 800 nm-wide nanogrooves were used.</P><P><B>Results</B></P><P>TDSCs from 5-week-old normal tendon showed high expression of type III collagen on the flat NOA86 substrate. In the 15-week normal tendon model, expression of type III collagen was high in TDSCs cultured on the 800 nm NOA86 substrates. However, in the 15-week tendon injury model, expression of type III collagen was similar irrespective of nanotopographic cues or substrate stiffness. The expression of type I collagen was also independent of nanotopographic cues and substrate stiffness in the 15-week normal and tendon injury models. Gene expression of scleraxis was increased in TDSCs cultured on the flat NOA86 substrate in the 5-week normal tendon model (<I>P</I>=0.001). In the 15-week normal tendon model, scleraxis was highly expressed in TDSCs cultured on the 800 nm and flat NOA86 substrate (<I>P</I>=0.043). However, this gene expression was not significantly different between the substrates in the 5-week tendinopathy and 15-week tendon injury models.</P><P><B>Conclusion</B></P><P>Development and maturation of tendon are enhanced when TDSCs from normal tendons were cultured on stiff surface, but not when the TDSCs came from pathologic models. Therapeutic applications of TDSCs need to be flexible based on tendon age and tendinopathy.</P>

A Food-Borne Outbreak Caused by Salmonella Enteritidis

Ozlem Tansel,Galip Ekuklu,Metin Otkun,Müşerref Tatman Otkun,Filiz Akata,Murat Tuğrul 연세대학교의과대학 2003 Yonsei medical journal Vol.44 No.2

This study was designed to define the epidemiology of a food-borne outbreak caused by Salmonella enteritidis that affected only one squadron of a military battalion located in the vicinity of the city of Edirne in Turkey. The outbreak was analyzed by a standard surveillance form of the Centers for Disease Control and Prevention. The relationship between the eaten foods and cases was analyzed by Fisher's exact chi-square test, and odds ratios were calculated by a case-control study. The outbreak affected 60 of 168 soldiers in the squadron, 16 of whom were hospitalized. S.enteritidis was cultured in stools from 13 of the hospitalized soldiers and from 3 soldiers who had prepared the food. All strains were completely susceptible to antibiotics; their plasmid profiles were also identical. The highest attack rate detected was 55.7% in an omelet eaten 24 hours before (p < 0.001). Furthermore, it was the riskiest food according to the case-control study (ORe because they had been discarded. All of the hospitalized cases recovered, and none of the control cultures of stools yielded the pathogen after three weeks. In conclusion, although our results didn't indicate the exact source of the outbreak microbiologically, the omelet was considered to be the source based on the epidemiological proofs.

일차수응력부식균열(PWSCC) 및 염화이온부식균열(CISCC) 저감용 표면개질기술 적용을 위한 코드케이스 개발

조성우,편영식,Nick Mohr,Jon Tatman,John Broussard,Jean Collin,이원근,오은종,장동현,구경회,황성식,최선웅,홍현욱 한국압력기기공학회 2019 한국압력기기공학회 논문집 Vol.15 No.1

In nuclear power plant operation and spent fuel canisters, it is necessary to provide a sound technical basis for the safety and security of long-term operation and storage respectively. Recently, the peening technology is being discussed and the technology will be adopted to ASME Section III, Division 1, Subsection NX (2019 Edition). The peening is prohibited in current edition, but it will be approved in 2019 Edition and adopted. However, Surface stress improvement techniques such as the peening is used to mitigate SCC susceptible in operating nuclear plants. Although the peening will be approved to ASME CODE, there are no performance criteria listed in the 2019 edition. The Korean International Working Group (KIWG) formed a new Task Group named “Advanced Surface Stress Improved Technology”. The task group will develop a CODE CASE to address PWSCC(Primary Water Stress Corrosion Cracking) and CISCC(Chloride Induced Stress Corrosion Cracking) for new ASME Section III components. TG-ASSIT was started to make peening performance criteria for ASME Section III (new fabrication) applications. The objective of TG-ASSIT is to gain consensus among the relevant Code groups that requirements/mitigation have been met.

Effect of self-assembled peptide–mesenchymal stem cell complex on the progression of osteoarthritis in a rat model

Kim, Ji Eun,Lee, Sang Mok,Kim, Soo Hyun,Tatman, Phil,Gee, Albert O,Kim, Deok-Ho,Lee, Kyung Eun,Jung, Youngmee,Kim, Sang Jun Dove Medical Press 2014 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.9 No.1

<P><B>Purpose</B></P><P>To evaluate the efficacy of mesenchymal stem cells (MSCs) encapsulated in self-assembled peptide (SAP) hydrogels in a rat knee model for the prevention of osteoarthritis (OA) progression.</P><P><B>Materials and methods</B></P><P>Nanostructured KLD-12 SAPs were used as the injectable hydrogels. Thirty-three Sprague Dawley rats were used for the OA model. Ten rats were used for the evaluation of biotin-tagged SAP disappearance. Twenty-three rats were divided into four groups: MSC (n=6), SAP (n=6), SAP-MSC (n=6), and no treatment (n=5). MSCs, SAPs, and SAP-MSCs were injected into the knee joints 3 weeks postsurgery. Histologic examination, immunofluorescent staining, measurement of cytokine levels, and micro-computed tomography analysis were conducted 6 weeks after injections. Behavioral studies were done to establish baseline measurements before treatment, and repeated 3 and 6 weeks after treatment to measure the efficacy of SAP-MSCs.</P><P><B>Results</B></P><P>Concentration of biotinylated SAP at week 1 was not significantly different from those at week 3 and week 6 (<I>P</I>=0.565). Bone mineral density was significantly lower in SAP-MSC groups than controls (<I>P</I>=0.002). Significant differences in terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling staining between the control group and all other groups were observed. Caspase-8, tissue inhibitor of metalloproteinases 1, and matrix metalloproteinase 9 were diffusely stained in controls, whereas localized or minimal staining was observed in other groups. Modified Mankin scores were significantly lower in the SAP and SAP-MSC groups than in controls (<I>P</I>=0.001 and 0.013). Although not statistically significant, synovial inflammation scores were lower in the SAP (1.3±0.3) and SAP-MSC (1.3±0.2) groups than in controls (2.6±0.2). However, neither the cytokine level nor the behavioral score was significantly different between groups.</P><P><B>Conclusion</B></P><P>Injection of SAP-MSC hydrogels showed evidence of chondroprotection, as measured by the histologic grading and decreased expression of biochemical markers of inflammation and apoptosis. It also lowered subchondral bone mineral density, which can be increased by OA. This suggests that the SAP-MSC complex may have clinical potential to inhibit OA progression.</P>