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      • Computer Simulation of Metal Surface Micro-Crack Inspection Using Pulsed Laser Thermography

        Tang Qingju,Bu Chiwu,Liu Yuanlin,Yu Fengyun,Zhao Yawei 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.3

        Surface micro cracks are easy to produce in the preparation and service process of metal material, which impacts on the safe operation of metal components. Pulsed laser spot excitation and infrared thermal imaging technology are combined to detect metal surface micro-cracks. The working principle of laser infrared thermal imaging detection technology was described. The three dimensional heat conduction model of pulsed laser excitation flux transfer in metal plate was established, and calculated using finite element method (FEM). The results showed that, thermal flow in the image is a “D” shape. There are temperature differences between the sound regions and defective regions, and the defects experiences the process of obscure, gradually clear, and gradually obscure. Pulsed infrared thermography sequence was processed by polynomial fitting method, and the coefficient images effectively improve the contrast between defective and non-defective areas, which is beneficial to the determination and of recognition micro cracks.

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        Synthesis of Polyamides Containing N-Methylpyrrole and N-Methylimidazole and Their Anticancer Activity

        Yuan, Gu,Xiao, Junhua,Huang, Weiqiang,Tang, Feili,Zhou, Yawei The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.5

        Three hairpin polyamides were designed and synthesized by a haloform reaction and DCC/HOBt coupling reaction without amino protection and deprotection. Their anticancer activity were investigated with three kinds of cell lines-hepatic carcinoma, lung carcinoma and gastric carcinoma, and the values of $IC_{50}$ were at range of $10^{-7}~10^{-8}M$.

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        Identification of HvLRX, a new dehydration and light responsive gene in Tibetan hulless barley (Hordeum vulgare var. nudum)

        Liang Junjun,Zhang Haili,Yi Ling,Tang Yawei,Long Hai,Yu Maoqun,Deng Guangbing 한국유전학회 2021 Genes & Genomics Vol.43 No.12

        Background Tibetan hulless barley (Hordeum vulgare var. nudum), adjusting to the harsh environment on Qinghai–Tibet Plateau, is a good subject for analyzing drought tolerance mechanism. Several unannotated diferentially expressed genes (DEGs) were identifed through our previous RNA-Seq study using two hulless barley accessions with contrasting drought tolerance. One of these DEGs, HVU010048.2, showed up-regulated pattern under dehydration stress in both drought tolerant (DT) and drought susceptible (DS) accessions, while its function in drought resistance remains unknown. This new gene was named as HvLRX (light responsive X), because its expression was induced under high light intensity while suppressed under dark. Objective To provide preliminary bioinformatics prediction, expression pattern, and drought resistance function of this new gene. Methods Bioinformatics analysis of HvLRX were conducted by MEGA, PlantCARE, ProtParam, CELLO et al. The expression pattern of HvLRX under diferent light intensity, dehydration shock, gradual drought stress, NaCl stress, polyethylene glycol (PEG) 6000 stress and abscisic acid (ABA) treatment was investigated by quantitative reverse transcription-polymerase chain reaction (RT-qPCR). The function of HvLRX was analyzed by virus induced gene silencing (VIGS) in hulless barley and by transgenic method in tobacco. Results Full cDNAs of HvLRX were cloned and compared in three hulless barley accessions. Homologues of HvLRX protein in other plants were excavated and their phylogenetic relationship was analyzed. Several light responsive elements (ATC-motif, Box 4, G-box, Sp1, and chs-CMA1a) were identifed in its promoter region. Its expression can be promoted under high light intensity, dehydration shock, gradual drought stress, PEG 6000, and NaCl stress, but was almost unchanged in ABA treatment. HvLRX-silenced plants had a higher leaf water loss rate (WLR) and a lower survival rate (SR) compared with controls under dehydration stress. The infected leaves of HvLRX-silenced plants lost their water content quickly and became withered at 10 dpi. The SR of HvLRX overexpressed transgenic tobacco plants was signifcantly higher than that of wild-type plants. These results indicated HvLRX play a role in drought resistance. Besides, retarded vegetative growth was detected in HvLRX-silenced hulless barley plants, which suggested that this gene is important for plant development. Conclusions This study provided data of bioinformatics, expression pattern, and function of HvLRX. To our knowledge, this is the frst report of this new dehydration and light responsive gene.

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