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Ngan, Luong Thi My,Moon, Joon-Kwan,Shibamoto, Takayuki,Ahn, Young-Joon American Chemical Society 2012 Journal of agricultural and food chemistry Vol.60 No.36
<P>An assessment was made of the growth-inhibiting, bactericidal, and urease inhibitory activities of paeonol (PA), benzoic acid (BA), methyl gallate (MG), and 1,2,3,4,6-penta-<I>O</I>-galloyl-β-<SMALL>d</SMALL>-glucopyranose (PGG) identified in Paeonia lactiflora root, structurally related compounds, and four antibiotics toward three reference strains and four clinical isolates of Helicobacter pylori using broth dilution bioassay and Western blot. BA and PA showed strong bactericidal effect at pH 4, while MG and PGG were effective at pH 7. These constituents exhibited strong growth-inhibiting and bactericidal activity toward the five strains resistant to amoxicillin (minimal inhibitory concentration (MIC) 12.5 mg/L), clarithromycin (64 mg/L), metronidazole (64 mg/L), or tetracycline (15 mg/L), indicating that these constituents and the antibiotics do not share a common mode of action. Structural characteristics, such as types of functional groups and carbon skeleton, and hydrophobicity appear to play a role in determining the anti-H. pylori activity. H. pylori urease inhibitory activity of PGG was comparable to that of acetohydroxamic acid, while MG was less potent at inhibiting urease than thiourea. The UreB band disappeared at 250 mg/L PGG on Western blot, while the UreA bands were faintly visible at 1000 mg/L PGG. These constituents showed no significant cytotoxicity. Global efforts to reduce the level of antibiotics justify further studies on P. lactiflora root-derived materials containing MG, PA, and PGG as potential antibacterial products or lead molecules for the prevention or eradication from humans from diseases caused by H. pylori.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2012/jafcau.2012.60.issue-36/jf3035034/production/images/medium/jf-2012-035034_0009.gif'></P>
Yeon Park, Jun,Young Kim, Hyun,Shibamoto, Takayuki,Su Jang, Tae,Cheon Lee, Sang,Suk Shim, Jae,Hahm, Dae-Hyun,Lee, Hae-Jeung,Lee, Sanghyun,Sung Kang, Ki Pergamon Press 2017 Bioorganic & medicinal chemistry letters Vol.27 No.17
<P><B>Abstract</B></P> <P>The biological activities of the ethanol extract from <I>Cirsium japonicum</I> var. <I>maackii</I> (ICF-1) and its major component, polyphenol cirsimaritin, were investigated as part of the search for possible alternative drugs for breast cancer. Three <I>in vitro</I> cell-based assays were used: the cell proliferation assay, tube-formation assay, and Western blot analysis. Both the ICF-1 extract and cirsimaritin inhibited the viability of HUVECs in a dose-dependent manner. The inhibition achieved was 36.89% at a level of 200μg/ml by the ICF-1 extract and 62.04% at a level of 100μM by cirsimaritin. The ICF-1 extract and cirsimaritin reduced tube formation by 12.69% at level of 25μg/ml and 32.18% at the levels of 6.25μM, respectively. Cirsimaritin inhibited angiogenesis by downregulation of VEGF, p-Akt and p-ERK in MDA-MB-231 cells, suggesting that cirsimaritin is potentially useful as an anti-metastatic agent. The present study demonstrated that <I>Cirsium japonicum</I> extract and its active component cirsimaritin is an excellent candidate as an alternative anti-breast cancer drug.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Analysis before and after Cooking Processes of a Trace Chlorpyrifos Spiked in Polished Rice
Lee, Su-Rae,Mourer, Charles R.,Shibamoto, Takayuki 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-
Polished rice grains were fortified with 500 ppb of chlorpyrifos. The fortified residues were analyzed by gas chromatography with a nitrogen-phosphorus detector or a Hall detector. Chlorpyrifos and its breakdown product 3,5,6-trichloro-2-pyridinol were recovered from fortified rice grains in the levels of 456 and 3.4 ppb, respectively. Washing rice grains with water removed approximately 60% of the chlorpyrifos residues. Fortified chlorpyrifos was found in cooked rice at a level of 130 ppb, which was approximately 30% of the original quantity. Acid treatment was required to remove chlorpyrifos bound to proteins or carbohydrates in the cooked rice.
Isolation and Identification of Headspace Volatiles Formed in Heated Butter
Lee, Su-Rae,Macku, Carlos,Shibamoto, Takayuki 이화여자대학교 생명과학연구소 1991 생명과학연구논문집 Vol.2 No.-
Unsalted sweet butter was heated at 100,150,or 200℃ for 5h. The headspace volatiles formed were collected during heating using a simultaneous purging and solvent extraction apparatus. Among 140 peaks on a gas chromatogram of the headspace sample from butter heated at 200℃, 77 were positively identified by gas chromatography and gas chromatograpy/ mass spectrometry. Major compounds identified were 21 aldehydes, 12 fatty acids, 11 ketones, 10 nitrogen-and /or sulfur-containing compounds, 7 alkanes, 6ð-lactones, and 4 furans, which constituted over 85% of total volatiles recovered. The number of volatiles formed in a headspace of butter heated at 100 or 150℃ was much less than that formed at 200℃. However, all volatiles formed at 100 and 150℃ was much less than that formed at 200℃. However, all volatiles formed at 100 and 150℃ were found in a sample heated at 200℃.
Ngan, Luong Thi My,Moon, Joon-Kwan,Kim, Jeong-Han,Shibamoto, Takayuki,Ahn, Young-Joon Springer 2012 World journal of microbiology biotechnology Vol.28 No.4
<P>The growth-inhibiting activities of Paeonia lactiflora (Paeoniaceae) root steam distillate constituents and structurally related compounds against nine harmful intestinal bacteria and eight lactic acid-producing bacteria were compared with those of two antibiotics, amoxicillin and tetracycline. Thymol, α-terpinolene, (-)-perilla alcohol and (1R)-(-)-myrtenol exhibited high to extremely high levels of growth inhibition of all the harmful bacteria, whereas thymol and α-terpinolene (except for Lactobacillus casei ATCC 393) inhibited the growth of all the beneficial bacteria (MIC, both 0.08-0.62 mg mL(-1)). Tetracycline and amoxicillin exhibited extremely high level of growth inhibition of all the test bacteria (MIC, <0.00002-0.001 mg mL(-1)). 1,8-Cineole, geraniol, (-)-borneol, (1S,2S,5S)-(-)-myrtanol, nerol, (S)-(-)-β-citronellol and ()-lavandulol also exhibited inhibitory activity but with differing specificity and levels of activity. Structure-activity relationship indicates that structural characteristics, such as geometric isomerism, degrees of saturation, types of functional groups and types of carbon skeleton, appear to play a role in determining the growth-inhibiting activity of monoterpenoids. Global efforts to reduce the level of antibiotics justify further studies on naturally occurring P. lactiflora root-derived materials as potential preventive agents against various diseases caused by harmful intestinal bacteria such as clostridia.</P>
Lee, Dahae,Ko, Hyeonseok,Kim, Young-Joo,Kim, Su-Nam,Choi, Kyung-Chul,Yamabe, Noriko,Kim, Ki Hyun,Kang, Ki Sung,Kim, Hyun Young,Shibamoto, Takayuki American Chemical Society 2016 Journal of agricultural and food chemistry Vol.64 No.4
<P>The effects of a red raspberry component, sanguiin H-6 (SH-6), on the induction of apoptosis and the related signaling pathways in A2780 human ovarian carcinoma cells were investigated. SH-6 caused an antiproliferative effect and a severe morphological change resembling that of apoptotic cell death but no effect on the cancer cell cycle arrest: In addition, SH-6 induced an early apoptotic effect and activation of caspases as well as the deavage of PARP, which is a hallmark of apoptosis. The early apoptotic percentages of A2780 cells exposed to 20 and 40 mu M SH-6 were 35.39 and 41.76, respectively. Also, SH-6 caused the activation of mitogen-activated protein, kinases (MAPKs), especially p38, and the increase of truncated p15/BID. These results in the present study suggest that the apoptosis of A2780 human ovarian carcinoma cells by SH-6 is mediated by the MAPK p38 and a caspase-8-dependent BID cleavage pathway.</P>