http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
이성도,정형복,임봉수,이제희,김유철,김효원,THANTHRIGETHIUNUWAN PRIYATHILAKA,W. D. Niroshana Wickramaarachchi,김세재,김신권 한국유전학회 2014 Genes & Genomics Vol.36 No.4
Arginine vasotocin (AVT) is a neurohypophysialhormone of non-mammal vertebrates functions in variousphysiological processes and homologous to mammalvasopressin. In this study, a full-length cDNA coding for aputative AVT precursor was isolated from flounder, Paralichthysolivaceus by rapid amplification of cDNA ends. Itwas 641 bp long, coding 153 amino acids, and consisted in69 bp long 50-untranslated region (UTR), 462 bp openreading frame and 110 bp 30-UTR. The putative amino acidsequence of flounder AVT was determined, and comparedwith other AVTs from different species. Also, 1,447 bp offlounder AVT gene, corresponding to the isolated cDNAregion with 2 introns within it, was isolated. Reverse transcriptionpolymerase chain reaction result showed thatflounder AVT was strongly expressed in the brain but not inthe any other tissues examined, confirming it is brain-specificgene. Expression profile of the AVT, together with arylalkylamineN-acetyltransferase, heat shock protein 70,interferon-stimulated gene 15 and natural resistance-associatedmacrophage protein genes were surveyed fromStreptococcus iniae infected flounders by real-time quantitativePCR. It revealed that flounder AVT is up-regulatedunder acute stress compared to the non-challenged control.