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RISS 인기검색어
- 검색키워드
- 저자 : T. Dennis Thomas (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
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Advances in Mulberry Tissue Culture
Thomas, T. Dennis 한국식물학회 2002 Journal of Plant Biology Vol.45 No.1
The mulberry (Morus spp.) is an important tree in the sericultural industry because its leaves constitute the sole source of food for the Mori silkworm (Bombyx mori). Qualitative and quantitative improvements in mulberry varieties play a vital role in industrial advances. However, the perennial nature of the plants, coupled with the species prolonged juvenile period, slows this process. Plant tissue-culture techniques have been used extensively for stock improvement During the last thirty years, several researchers have reported success in plant regeneration from different explants types. This review describes the major findings in mulberry tissue-culture research.
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Direct Somatic Embryogenesis of Curculigo orchioides Gaertn., an Endangered Medicinal Herb
Thomas, T.Dennis,Jacob, Alphonsa The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.3
In vitro multiplication of Curculigo orchioides was achieved by direct somatic embryogenesis in young leaf segments. Immature leaf segments of about 0.5 cm in length were cultured on MS medium supplemented with different concentrations of BAP (2-10 $\mu{M}$) or Kin (2-10 $\mu{M}$). Optimum response in terms of per cent cultures responding (89%) and the number of embryos per explant (16) were observed on MS medium supplemented with 8 $\mu$M BAP. The emergence of several somatic embryos on the adaxial side of the leaf segments was observed one month after the culture. Germinated somatic embryos were grown up to about 1.5 cm length before transferring to maturation medium. For maturation, the individual embryos were isolated and transferred to MS medium supplemented with BAP (5 $\mu{M}$) and NAA (0.5 $\mu{M}$). The plantlets emerged from the embryos were transferred to soil containing 1 peat: 1 sand with 90% success. The embryos were formed directly on the leaf segments without any callus phase. Direct regeneration of somatic embryos is important for the conservation of this endangered species, as rare somaclonal variants are likely to arise than from indirect regeneration.
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T. Dennis Thomas,Surabhi Shankar 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.1
An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA (0.5–8 lM) or Kn (0.5– 8 lM) alone or in combination with NAA (0.5–1.5 lM). Maximum multiple shoot induction was observed on MS medium supplemented with 4 lM BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of 1 lM NAA along with 4 lM BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA (5 lM) and 2,4-D (2 lM). The callus was subcultured on MS medium supplemented with BA (2–15 lM) or Kn (2–15 lM) alone or in combination with NAA (0.5–2 lM) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with 10 lM BA and 1 lM NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on halfstrength MS medium supplemented with NAA (1–7 lM) or IBA (1–7 lM). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant. An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA (0.5–8 lM) or Kn (0.5– 8 lM) alone or in combination with NAA (0.5–1.5 lM). Maximum multiple shoot induction was observed on MS medium supplemented with 4 lM BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of 1 lM NAA along with 4 lM BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA (5 lM) and 2,4-D (2 lM). The callus was subcultured on MS medium supplemented with BA (2–15 lM) or Kn (2–15 lM) alone or in combination with NAA (0.5–2 lM) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with 10 lM BA and 1 lM NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on halfstrength MS medium supplemented with NAA (1–7 lM) or IBA (1–7 lM). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success. This standardized protocol will help to conserve this rare medicinal plant.
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High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
Puthur, Jos T.,Thomas, T. Dennis 한국식물학회 2004 Journal of Plant Biology Vol.47 No.1
An effective and reproducible protocol for the micropropagation of Kigelia pinnata L through high frequency callus regeneration is described. Seeds were surface sterilized before culturing on Gamborgs basal medium (B5 medium). After two weeks the cotyledonary node along with a portion of the hypocotyl were carefully excised from well-developed embryos and subcultured on B5 medium supplemented with different concentrations of 2,4-D and BAP. The cultured cotyledonary node explants showed callus formation at the base of the lower cut end of the hypocotyl. This callus showed shoot initiation after two weeks of subculture on the regeneration medium supplemented with various concentrations of BAP alone or in combination with NAA. The highest number of shoot regeneration occurred on medium containing 5 μM BAP and 0.1 μM NAA. The optimum rooting of the regenerated shoots was observed on %B5 medium supplemented with 4 μM IBA. Micropropagated plants were successfully established in soil in field condition with a survival frequency of 100%.
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