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Dahee KIM,Ju-Hyun YU,Kyung-Sik HONG,Chan-Duck JUNG,Hoyong KIM,Jungbae KIM,Suwan MYUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The production of value-added products such as xylooligosaccharides (XOS) from lignocellulosic biomasses using eco-friendly refining technologies has become increasingly important. In this study, high-concentration XOS from empty fruit bunch (EFB) was produced on a pilot scale using twin-screw extrusion, continuous hydrothermal treatment, and membrane-based concentration in sequence. And laboratory-scale optimization using the xylanases-based hydrolysis of hemicellulose hydrolysates was performed to produce high content of xylobiose and xylotriose, which are the most suitable nutritive materials for the growth and activity of Bifidobacterium and Lactobacillus brevis spp. The xylobiose and xylotriose under the optimized conditions were acquired on the pilot scale. Furthermore, a novel approach involving a combination of enzyme-mediated radical polymerization and ultrafiltration-membrane-based separation was applied to purify XOS by removing the undesired side products such as phenolic compounds. As a result, more than half of the total phenolic compounds in the crude XOS were removed, and the developed process was proved to be effective.