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RNA-based dynamic genetic controllers: development strategies and applications
Jang, Sungho,Jang, Sungyeon,Yang, Jina,Seo, Sang Woo,Jung, Gyoo Yeol Elsevier 2018 Current opinion in biotechnology Vol.53 No.-
<P>Dynamic regulation of gene expression in response to various molecules is crucial for both basic science and practical applications. RNA is considered an attractive material for creating dynamic genetic controllers because of its specific binding to ligands, structural flexibility, programmability, and small size. Here, we review recent advances in strategies for developing RNA-based dynamic controllers and applications. First, we describe studies that re-engineered natural riboswitches to generate new dynamic controllers. Next, we summarize RNA-based regulatory mechanisms that have been exploited to build novel artificial dynamic controllers. We also discuss computational methods and high-throughput selection approaches for <I>de novo</I> design of dynamic RNA controllers. Finally, we explain applications of dynamic RNA controllers for metabolic engineering and synthetic biology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Unique properties of RNA lead to the development of RNA-based dynamic genetic controllers. </LI> <LI> Natural riboswitches are re-engineered to detect new molecules. </LI> <LI> RNA-based regulatory mechanisms are exploited to construct novel dynamic RNA controllers. </LI> <LI> Computational methods and <I>in vitro</I>–<I>in vivo</I> selection enable <I>de novo</I> design of dynamic RNA controllers. </LI> <LI> Dynamic RNA controllers are utilized for metabolic engineering and synthetic biology. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>