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Sunggil Kim,Kil Sun Yoo,Leonard M. Pike 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.4
Bulb color is an important trait in onion (Allium cepa) breeding, but the lack of a method to quantify red color intensity in a large number of samples has hindered assessment of essential breeding parameters such as heritability. We developed a new method based on digital imaging that allows facile quantification of red color intensity in a large number of samples. The brightness of dry skin in digital images was measured using the histogram function of Adobe<SUP>®</SUP> Photoshop<SUP>®</SUP> and showed highly significant correlation with both visual rating of red intensity and anthocyanin content. This method was utilized to assess the uniformity of red color intensity of doubled haploid (DH) lines and location effects on red color development. Three red DH lines, four pink F₁ hybrids, and three red commercial cultivars were grown at two locations with three replications. DH lines showed less variation of color intensity than commercial cultivars. A significant location effect on the red color intensity was observed in combined analysis of variance from two locations.
Sunggil Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
To reveal the linkage relationship between the Ms locus, a restorer-of-fertility gene for cytoplasmic male-sterility (CMS) caused by CMS-S cytoplasm in onion (Allium cepa L.) and previously reported molecular markers linked to the Ms locus, 11 recombinants selected from 4,273 segregating plants originating from the cross between male-sterile maternal and male-fertile paternal lines were analyzed. Results showed that genotypes of a codominant marker, jnurf12, were perfectly matched with the male-fertility phenotypes in all recombinants, but that this marker was not applicable in diverse breeding lines due to multiple band patterns. For the development of more reliable markers, a 12-bp indel was identified from the sequences which were obtained by genome walking, and was used to develop a simple PCR marker which was designated jnurf13. When 104 diverse breeding lines containing CMS-S cytoplasm were analyzed with the jnurf13 marker, male-fertility phenotypes of all breeding lines were perfectly matched with marker genotypes. To our surprise, phenotypes of 153 breeding lines containing CMS-T-like cytoplasm were also matched with genotypes of the jnurf13 marker which was linked to the Ms locus for the CMS-S system. Furthermore, phenotypes of four F2 populations containing CMS-T-like cytoplasm co-segregated perfectly with jnurf13 genotypes. Allelic segregation distortion was detected in two F2 populations using the jnurf13 maker. The results of this study were in conflict with a previous model for inheritance of fertility restoration in the CMS-T system. Therefore, we proposed a new model based on the data analyzed with the jnurf13 marker, which was in linkage disequilibrium with restorer-of-fertility genes for both CMS systems.