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Cho, Eun Sook,Jin, Byung Rae,Sohn, Hung Dae,Choi, Kwang Ho,Kim, Soung Ryul,Kang, Seok Woo,Yun, Eun Young,Kim, Sang Hyun,Kim, Keun Young,Je, Yeon Ho,Kang, Seok Kwon 한국잠사학회 1998 한국잠사곤충학회지 Vol.40 No.2
To construct transfurmed Bm5 cells, Autographa californica nuclear polyhedrosis virus(AcNPV) IE1 gene, an immediate early viral gene was firstly used in this study. AcNPV IE1 gene, which shares on 95.3% nucleotide sequence homology with Bombyx mori nuclear polyhedrosis virus (BmNPV) IE1 gene, was isolated and cloned into pBluescript. Neomycin gene from pKO-neo was inserted under the control of the IE1 promoter to yield pAcIE1-neo. The plasmid pAcIE1-neo was transfected into Bm5 or Sf9 cells, and neomycin-resistant cells were selected in TC100 medium containing 10% fetal bovine serum (FBS) and 1 mg/ml G4l8 for two weeks. Individual clones were picked and each was amplified for further characterization. The genomic DNA from neomycin-resistant cells was isolated and characterized by PCR using AcNPV IEI gene-specific primers and by Southern blot analysis using neomycin gene probe. We concluded that AcNPV IE1 gene was functional in B. mori-derived Bm5 cells as well as Spodoptera fugjprrda-derived Sf9 cells to produce stably-transformed insect cells
Sang Jun Park,권병석,김혜정,이종수,Hong Rae Cho 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.6
CD137 (also called 4-1BB and TNFRSF9) has recently received attention as a therapeutic target for cancer and a variety of autoimmune and inflammatory diseases. Stimulating CD137 in vivo enhances CD8+ T cell-activity and results in strong immunosuppression in some contexts. This paradoxical phenomenon may be partially explained by the ability of CD137-stimulating reagents (usually agonistic monoclonal antibodies against CD137) to over-activate T cells and other CD137-expressing cells. This over-activity is associated with deleting pathogenic T cells and B cells or generating a tolerogenic microenvironment. Recent studies, however, suggest that the biology of CD137 and its ligand (CD137L) are more complex, mainly due to bidirectional signaling between CD137 and CD137L. For example, signaling through CD137L in non-hemato-poietic cells such as epithelial cells and endothelial cells has been shown to play an essential role in sterile inflammation by regulating immune cell recruitment. One outstanding, and clinically important, issue is understanding how bidirectional signaling through CD137 and CD137L controls the vicious cycle of sterile inflammation (e.g., ischemia-reperfusion tissue injury and meta-inflammatory diseases).
Cho, Eun Jeong,Chun, Sung-Min,Park, Hwan,Sung, Chang Ohk,Kim, Kyu-Rae Elsevier 2020 Gynecologic oncology Vol.157 No.1
<P><B>Abstract</B></P> <P><B>Objective</B></P> <P>Genomic characteristics of gestational trophoblastic neoplasm (GTN) are mostly unknown. This study reveals the molecular features of malignant GTN, including choriocarcinoma (CC), epithelioid trophoblastic tumor (ETT), and placental site trophoblastic tumor (PSTT), by whole transcriptome sequencing analysis.</P> <P><B>Methods</B></P> <P>Data obtained from the total RNA sequencing of 2 CC, 4 ETT, and 4 PSTT were evaluated for differential gene expression, pathway alteration, fusion gene, infiltrating immune cell type, PD-L1 and <I>PTEN</I> expression level, and mutation analysis was performed.</P> <P><B>Results</B></P> <P>The transcriptome data were correlated with known biomarkers, including <I>HDS3B1</I>, p63, hCG, and hPL for all tumor types. ETT and PSTT were more closely clustered compared with CC in clustering analysis using gene expression; however, ETT showed various altered signaling pathways, including PI3K-Akt-mTOR, with frequent loss of PTEN protein expression. This finding was both well correlated with <I>PIK3CA</I> c.3140A > G pathogenic mutation, detected in 1 ETT, and further confirmed using the MassARRAY method. PSTT showed an overexpressed gene cluster associated with muscle contraction and G protein-coupled receptor activity. No significant fusion gene was seen in all 10 cases. In tumor-infiltrating immune cell profiles, CD4 memory T cell and macrophage signature were relatively high in ETT and PSTT. PD-L1 mRNA expression level was high in all cases, which was significantly correlated with the PD-L1 level by immunohistochemistry (<I>p</I> = 0.03) with positivity in all 10 cases.</P> <P><B>Conclusions</B></P> <P>ETT and PSTT were similar at the transcriptome level, with a high level of PD-L1 expression in all tumor types; however, specific pathways, such as PI3K signaling, were altered in ETT.</P> <P><B>Highlights</B></P> <P> <UL> <LI> ETT and PSTT are closely linked in whole transcriptome level. </LI> <LI> ETT is altered in PI3K-AKT-mTOR signaling pathway. </LI> <LI> ETT harbors <I>PIK3CA</I> mutation. </LI> <LI> ETT, PSTT, and CC show a variable high expression level of PD-L1. </LI> </UL> </P>
Cho, Sang-Rae,Kim, Ui-Hyung,Kumar, Kuldeep,Lee, Seung-Hwan,Lee, Myeung-Sik,Kim, Hyeon-Shup,Lee, Hyun-Jae,Yang, Byoung-Chul The Korean Society of Embryo Transfer 2014 한국동물생명공학회지 Vol.29 No.4
The objective of this study was to investigate the interrelationship of weight and hormonal changes through the administration of vitamin A to first time pregnant heifers. A total of 28 Korean heifers was used for this study. The heifers were divided into two groups - with vitamin A (n=14) and without vitamin A (control) (n=14) in the feed. Body weight increased in vitamin A treated heifers starting 9 months until 15 months. After pregnancy, vitamin A treated heifers were found to maintain higher body weights than the control group. Pre-pregnancy and post pregnancy progesterone levels were not different between the two groups. Serum estradiol levels of heifers at different growth stages showed relatively higher E2 levels than the control. Also, the control during pregnancy may show higher serum E2 levels than the vitamin A treated heifers. The growth phase serum estradiol levels in heifers may be relatively higher than the control. During pregnancy it showed a similar trend. Serum levels of vitamin A treated heifers did not differ from pregnant heifers at 5 months of age. However, after 5 months from conception until 8 months of treatment it showed a high level. Serum cholesterol in pregnant cows was higher in the control group than the treatment from beginning until the end of pregnancy. This is considered to be related to fetus development during pregnancy, as well as the mother's health.
Sung-Sik Kang,Ui-Hyung Kim,Jun Sang Ahn,Jeong Il Won,Sang-Rae Cho 한국동물생명공학회(구 한국동물번식학회) 2021 Journal of Animal Reproduction and Biotechnology Vol.36 No.2
In the present study, we examined if deep uterine artificial insemination (DUAI) can improve the pregnancy rate of artificial insemination (AI) using epididymal spermatozoa (ES) in Hanwoo cattle. The estrus cycles of 88 Hanwoo cows were synchronized, and 17 cows were artificially inseminated using the DUAI method with ES, 20 cows were artificially inseminated via the uterine body (BUAI) method with ES, and as a control, 51 cows were inseminated by using the BUAI method with ejaculated spermatozoa from 1 proven bull after frozen thawing. The pregnancy rate of the DUAI method (58.8%) was higher than that of the BUAI method (25.0%, p = 0.0498). The motility of ES was examined immediately after thawing and after 3 and 6 h of incubation. The rapid progressive sperm motility of the control group was significantly higher than that of the ES group immediately after thawing and after 3 and 6 h of incubation (p < 0.05). The straight line velocity and average path velocity of the ES group after 6 h of incubation were significantly lower than those in the control group (p < 0.05). The linearity and amplitude of lateral head of ES were lower than those at 6 h (p < 0.05). The flagellar beat cross frequency and hyperactivation of ES were lower than the control spermatozoa immediately after thawing and at 3 h (p < 0.05). These motility parameters suggested that ES had a low motility and fertilization ability compared to the control spermatozoa. After frozen-thawing and 3 h of incubation, the percentage of live spermatozoa with intact acrosomes in the ES was significantly lower than that in ejaculated spermatozoa (p < 0.05). Our findings suggested that the DUAI method can overcome the low pregnancy rate of ES, despite the low motility, viability, and fertilization ability of ES.
Development of a Camera Self-calibration Method for 10-parameter Mapping Function
Sung-Min Park,Chang-je Lee,Dae-Kyeong Kong,Kwang-il Hwang,Deog-Hee Doh,Gyeong-Rae Cho 한국해양공학회 2021 韓國海洋工學會誌 Vol.35 No.3
Tomographic particle image velocimetry (PIV) is a widely used method that measures a three-dimensional (3D) flow field by reconstructing camera images into voxel images. In 3D measurements, the setting and calibration of the camera"s mapping function significantly impact the obtained results. In this study, a camera self-calibration technique is applied to tomographic PIV to reduce the occurrence of errors arising from such functions. The measured 3D particles are superimposed on the image to create a disparity map. Camera self-calibration is performed by reflecting the error of the disparity map to the center value of the particles. Vortex ring synthetic images are generated and the developed algorithm is applied. The optimal result is obtained by applying self-calibration once when the center error is less than 1 pixel and by applying self-calibration 2–3 times when it was more than 1 pixel; the maximum recovery ratio is 96%. Further self-correlation did not improve the results. The algorithm is evaluated by performing an actual rotational flow experiment, and the optimal result was obtained when self-calibration was applied once, as shown in the virtual image result. Therefore, the developed algorithm is expected to be utilized for the performance improvement of 3D flow measurements.