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민관기,표석능,이동권 성균관대학교 약학연구소 1999 成均藥硏論文集 Vol.11 No.-
Abstract-Streptococcus pneumoniae (pneumococcus) is the most frequent causative agent of acute bacterial pneumonia. Outstanding characteristic of pneumococcus is an ample polysaccharide capsule that is highly antigenic agent and is the major factor for classification of pneumococcus into more than 94 serotypes. In this study, production of capsular polysaccharide from Streptococcus pneumoniae type 23 was optimized by supplementation of metal ions or by modulation of culture conditions. When brain heart infusion (BHI) broth was supplemented with 1 mM concentration of CaCl_2, CuSO_4 and MgSO_4, growth of pneumococcus as well as polysaccharide yield were stimulated. Also slight aeration gave rise to better polysaccharide yield.
김수남,이동권,표석능 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1
Streptococcus pneumoniae remains a major cause of bacteremia, meningitis, penumonia, sinusitis, and otitis media. Although this organism was originally exquisitely susceptible to penicillin, the last two decades have witnessed the emergence of strains resistant to penicillin as well as other antimicrobial agents in many parts of the world. 102 isolates obtained from patients with invasive pneumococcal disease have been examined the susceptibility to various antibiotics. 52% to 85% of isolates tested were resistant to various antibiotics. These data suggest that more powerful antibiotics should be used for the treatment of pneumococal infection.
(Suhk Neung Pyo),(Byung Oh Kim) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.3
N/A Helicobacter pylori is a major cause of gastric-associated diseases. In our previous study, the Adhesin/CTXA2B was expressed as insoluble recombinant chimeric protein derived from the H. pylori adhesin genetically coupled to CTXA2B subunit in Escherichia coli. Since it is very important to optimize IPTG concentration, culture temperature and composition of medium to maximize cell growth and productivity, these conditional growth factors were determined for increasing the productivity of the expressed Adhesin/ CTXA2B chimeric protein in Escherichia coli JM 101 carrying pTEDhpa/ctxa2b. Our data demonstrate that optimal medium for increased production of chimeric protein was a YCP/Glu medium composed of 2% yeast extract, 1% casamino acid, phosphate solution [0.3% KH₂P0₄, 0.4% Na₂HPO₄, 0.25% (NH₄)₂HPO₄], and 0.5% glucose. In addition, optimal concentration of IPTG was 1 mM and culture temperature, 37℃.
Levels of Viral Glycoprotein Provide a Measure of Modulated Chemotherapeutic Effect
Pyo, Suhk Neung,Shin, Jae Yong,Yoon, Yeon Sook 한국응용약물학회 1999 Biomolecules & Therapeutics(구 응용약물학회지) Vol.7 No.3
A chemosensitivity assay with small replicate Mm5mt/cl C3H mammary tumor cell cultures was developed to determine whether changes in viral antigen expression and release into culture fluids could be utilized as an in vitro measure of modulating drug effect. The 52,000 MW viral envelope glycoprotein (gp52) of the mouse mammary tumor virus (MMTV) was measured in culture fluids of control and drug-treated cultures while cell density was simultaneously determined by cell staining and OD 664 nm determination. While extracellular gp52 levels and cell density progressively increased over 72 hours for control cultures, declines in both parameters provided dual measures of effect for combination [N(phophonacetyl-L-aspartic acid)+ 5-fluorouracil], combination [N(phophonacetyl-L-aspartic acid) + 5-fluoro-5'-deoxyuridine] and single component treatment of this combination. At each treated time point, these combinations begin to produce a greater decline in both cell density and gp52 levels as compared to single drug treatments. These results indicate that N(phophonacetyl-L-aspartic acid) in combination can enhance the effectiveness of single drug.
Characterization of dnaK Mutants in Streptococcus pneumoniae
Pyo,Suhk-Neung,Kim,Seung-Whan,Rhee,Dong-Kwon The Korea Science and Technology Center 2000 BMB Reports Vol.33 No.1
DnaK is a major heat shock protein and known to be highly conserved in all species. Previously, the dnaK in Streptococcus pneumoniae was cloned and the immunogenic nature characterized. In this study, dnaK mutants were generated by insertion of duplication mutagenesis and their characteristics examined. They had defective growths at all temperatures (20℃-42℃) and cell divisions, and formed filaments after a temperature shift from 30 to 42. A unique feature of the dnaK mutants of S. pneumoniae, unlike those of E. coli and B. subtilis, was the growth capability at high temperature (42℃) without producing the putative GroES. Our results suggest that DnaK may serve as a regulator and/or modifier in GroEL gene expression.
Kang, Nam-Sung,Pyo, Suhk-Neung,Sohn, Eun-Hwa The Korean Society of Food Science and Nutrition 2010 Preventive Nutrition and Food Science Vol.15 No.1
This study examined the effects of Acrylamide (ACR) and L-ascorbic acid (AsA) on the proliferation of splenocytes and the mitogen-stimulated lymphocyte proliferation in young (8 weeks) and aged (82 weeks) C57BL/6male mice in vitro. AsA increased splenocyte proliferation in both groups; however, this effect was higher in old mice, while the proliferation of lymphocyte was decreased except for treatment at $1\;{\mu}g/mL$ low concentration in both mice. In addition, ACR treatment resulted in decreased LPS-induced B lymphocyte proliferation and ConA-induced T lymphocyte proliferation in both groups. However, AsA increased LPS/ConA-induced lymphocyte proliferation in young groups and had no effects in old mice except at $0.5\;{\mu}g/mL$ Thus, the present data indicate that there is no difference effect of ACR and AsA on lymphocyte proliferation, whereas the effect of AsA on mitogen-induced cell proliferation was reduced in old mice. Overall, our results suggest that various immunomodulators have differing effects of lymphocytic proliferation on young versus aged mice.
Characterization of dnaK Mutants in Streptococcus pneumoniae
Kim, Seung-Whan,Pyo, Suhk-Neung,Rhee, Dong-Kwon Korean Society for Biochemistry and Molecular Biol 2000 Journal of biochemistry and molecular biology Vol.33 No.1
DnaK is a major heat shock protein and known to be highly conserved in all species. Previously, the dnaK in Streptococcus pneumoniae was cloned and the immunogenic nature characterized. In this study, dnaK mutants were generated by insertion of duplication mutagenesis and their characteristics examined. They had defective growths at all temperatures ($20^{\circ}C-42^{\circ}C$)and cell divisions, and formed filaments after a temperature shift from 30 to 42. A unique feature of the dnaK mutants of S. pneumoniae, unlike those of E. coli and B. subtilis, was the growth capability at high temperature ($42^{\circ}C$) without producing the putative GroEL. Our results suggest that DnaK may serve as a regulator and/or modifier in GroEL gene expression.