Impact of Nosema sp. Infection on Nutritional Physiology and Growth of the Tasar Silkworm Antheraea mylitta

( Sudhansu Sekhar Rath ),( Mrinal Kanti Singh ),( Suryanarayana N ) 한국잠사학회 2009 International Journal of Industrial Entomology Vol.19 No.2

Nosema sp. infection in the Indian tasar silkworm, Antheraea mylitta exerts a complex of influences on its host. The instar duration was extended significantly (P<0.001) except in 1st instar. The infected larvae took about 48 days to reach the spinning stage against 40 days in the uninfected ones. The final weight attained by the larva at the end of each instar of development declined significantly following infection, as did weight gain and relative growth rate (RGR). The growth recorded/ day declined in infected larvae compared to uninfected ones from 8.2% during 1st instar to 43.3% during 5th instar. Food ingestion and digestion increased with advancement of the instar significantly irrespective of the status of the larvae but the relative consumption rate (RCR) declined. These parameters significantly declined in infected larvae (except food digested during 2nd instar). The decline was more during 3rd instar. In contrast, the approximate digestibility (AD %) was significantly higher in infected larvae than uninfected ones leaving the 1st instar larvae unaffected. The efficiency of conversion of ingested food (ECI) and efficiency of conversion of digested food (ECD) did not change in a patterned way following the microsporidia (Nosema sp.) infection. The values of ECI significantly changed during 2nd, 3rd and 5th instars; while the change in ECD during 2nd, 4th and 5th were significant. During the entire larval life all the parameters tends to decline significantly following microsporidia infection but AD registered a significant increase. Nosema sp. spore concentration has increased 270.7 times during larval development in the course of experimentation.

Computational fluid dynamics analysis of flow through immobilized catalyzed packed bed reactor for removal of 4-chlorophenol from wastewater

Sudhansu Sandhibigraha,Soumya Sasmal,Tarun Kanti Bandyopadhyay,Biswanath Bhunia 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

The computational fluid dynamics (CFD) simulation of the packed bed reactor (PBR) was carried out using ANSYS Fluent software. The various process parameters, such as inlet concentration of 4-chlorophenol (4-CP), flow rate, bed height, and porosity, were optimized to predict maximum biodegradation of 4-CP in immobilized catalyzed PBR. The geometrical mesh of the PBR was constructed using Gambit software, and a mesh size of 236995 was selected from the grid-independent study. A laminar flow model was used to understand the hydrodynamics as well as concentration profile of 4-CP inside the PBR using Fluent software. Through CFD, the effect of the flow rate, inlet concentration, and the bed height and porosity of the immobilized catalyst bed on the static pressure, mass imbalance, velocity, and stress-strain field inside the PBR was visualized. CFD simulation study predicted that maximum biodegradation of 4-CP was found in the presence of 500 mg/L of inlet concentration of 4-CP, 4 mL/min of flow rate, 18 cm of bed height and 0.375 of porosity. An experimental study was conducted for wastewater flow through the B. subtilis MF447840.1 immobilized catalyzed PBR to remove the 4-CP in the laminar flow region. It was evident that CFD simulated results agreed well with experimental values.

Impact of Quantitative Feeding on Nutritional Parameters of Fifth Instar Larvae of Antheraea mylitta

( Sudhansu Sekhar Rath ) 한국잠사학회 2010 International Journal of Industrial Entomology Vol.20 No.2

Feeding is an important aspect of insect nutrition which in turn supports growth, development, reproduction and survival. The impact of quantitative feeding on nutritional parameters of fifth instar Antheraea mylitta larvae was studied by providing fresh leaves of Terminalia tomentosa for 1 to 4 times a day. All the parameters improved upon providing fresh diets more times over the single diet (1FD). The absolute body weight increased with increase in number of feeds/day significantly (P<0.001). While the maximum weight recorded was 29.61 g in 1FD, the respective weights for two feeds/day (2FD), three feeds/day (3FD) and four feeds/day (4FD) were 39.41 g, 40.63 g and 42.66 g respectively. Feeding period and instar duration were declined (P<0.001) and survival increased significantly (P<0.001) upon increase in the number of diets. Nutritional indices like ingestion, digestion, relative consumption rate (RCR), relative growth rate (RGR) and gain in body weight increased significantly with the increase in number of feeds/day, but approximate digestibility (AD) and efficiency of conversion of ingested food (ECI) declined, while efficiency of conversion of digested food (ECD) did not change.

A kinetic study of 4-chlorophenol biodegradation by the novel isolated Bacillus subtilis in batch shake flask

Sudhansu Sandhibigraha,Sagnik Chakraborty,Tarunkanti Bandyopadhyay,Biswanath Bhunia 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.1

Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolated bacterial strain was phylogenetically related with B. subtilis. This strain was capable of breaking down both phenol and 4-CP at the concentration of 1,000 mg/L. Also, the isolated strain can able to metabolize five diverse aromatic molecules such as 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 4-nitrophenol, and pentachlorophenol for their growth. An extensive investigation was performed to portray the kinetics of cell growth along with 4-CP degradation in the batch study utilizing 4-CP as substrate. Various unstructured models were applied to evaluate the intrinsic kinetic factors. Levenspiel’s model demonstrates a comparatively enhanced R² value (0.997) amongst every analyzed model. The data of specific growth rate (μ), saturation constant (KS), and YX/S were 0.11 h<SUP>-1</SUP>, 39.88 mg/L, along with 0.53 g/g, correspondingly. The isolated strain degrades 1,000 mg/L of 4-CP within 40 h. Therefore, B. subtilis MF447840.1 was considered a potential candidate for 4-CP degradation.

Male Mating History in Antheraea mylitta and its Effect on Ejaculation Size and Female Reproductive Fitness

( Sudhansu Sekhar Rath ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.22 No.2

Reproductive activity in the economically important insect, Antheraea mylitta is very important because the female reproduces only once in her life time and the aging is very rapid and costly. It is a capital breeder and strictly a monoandrous species. To know how strategically the insect behaves and the male allocates its ejaculate resource more prudently during its successive mating with virgin females to maximize its own fitness and the fitness of the female with whom it mated, both being most important to sericulture industry. So, the present study was undertaken and the results revealed fresh and virgin females always prefer to mate with fresh virgin males (84%) and receives high dose of ejaculates leading to higher hatchability than to virgin males of one day old (13.7%) and one day old males with mating experience (2.3%). The ejaculation size (as referred to eupyrene sperm count in the ejaculation) declined significantly over successive mating (r = -0.9931, P < 0.001), so also the male body weight (r = -0.9560, P < 0.001). The quantity of ejaculate passed to female also dramatically declined during aging (r = -0.9982, P < 0.001). It was found that male weight contribute substantially to the quantum of ejaculate (r = -0.9519, P < 0.001), so also higher fecund females receive relatively more ejaculate than the lower group to reach higher reproductive fitness. The life time fecundity was found to be 334±31.

Study of surface roughness and flank wear in hard turning of AISI 4140 steel with coated ceramic inserts

Sudhansu Ranjan Das,Debabrata Dhupal,Amaresh Kumar 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.10

This experimental investigation deals with dry hard turning of AISI 4140 steel using PVD-TiN coated Al2O3+TiCN mixed ceramic inserts. The combined effect of cutting parameters (cutting speed, feed and depth of cut) on performance characteristics such as surfaceroughness and flank wear is explored by Full factorial design (FFD) and analysis of variance (ANOVA). The results show that feed is theprincipal cutting parameter influencing surface roughness, followed by cutting speed. However, flank wear is affected by the cuttingspeed and interaction of feed-depth of cut, although depth of cut has not been found statistically significant, but flank wear is an increasingfunction of depth of cut. Observations are made on the machined surface, and worn tool by Scanning electron microscope (SEM) toestablish the process. Abrasion was the major wear mechanism found during hard turning within the studied range. The effect of toolwear on surface roughness was also studied. The experimental data were analyzed to predict the optimal range of surface roughness andflank wear. Based on Response surface methodology (RSM), mathematical models were developed for surface roughness (Ra) and flankwear (VB) with 95% confidence level. Finally, under optimum cutting conditions (obtained by response optimization technique), tool lifewas evaluated to perform cost analysis for justifying the economic viability of coated ceramic inserts in hard turning. The estimated machiningcost per part for TiN coated ceramic was found to be lower (Rs. 12.31) because of higher tool life (51 min), which results in thereduction of downtime and increase in savings.

Impact of Quantitative Feeding on Nutritional Parameters of Fifth Instar Larvae of Antheraea mylitta

Rath, Sudhansu Sekhar Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.20 No.2

Feeding is an important aspect of insect nutrition which in turn supports growth, development, reproduction and survival. The impact of quantitative feeding on nutritional parameters of fifth instar Antheraea mylitta larvae was studied by providing fresh leaves of Terminalia tomentosa for 1 to 4 times a day. All the parameters improved upon providing fresh diets more times over the single diet (1FD). The absolute body weight increased with increase in number of feeds/day significantly (P<0.001). While the maximum weight recorded was 29.61 g in 1FD, the respective weights for two feeds/day (2FD), three feeds/day (3FD) and four feeds/day (4FD) were 39.41 g, 40.63 g and 42.66 g respectively. Feeding period and instar duration were declined (P<0.001) and survival increased significantly (P<0.001) upon increase in the number of diets. Nutritional indices like ingestion, digestion, relative consumption rate (RCR), relative growth rate (RGR) and gain in body weight increased significantly with the increase in number of feeds/day, but approximate digestibility (AD) and efficiency of conversion of ingested food (ECI) declined, while efficiency of conversion of digested food (ECD) did not change.

Male Mating History in Antheraea mylitta and its Effect on Ejaculation Size and Female Reproductive Fitness

Rath, Sudhansu Sekhar Korean Society of Sericultural Science 2011 International Journal of Industrial Entomology Vol.22 No.2

Reproductive activity in the economically important insect, Antheraea mylitta is very important because the female reproduces only once in her life time and the aging is very rapid and costly. It is a capital breeder and strictly a monoandrous species. To know how strategically the insect behaves and the male allocates its ejaculate resource more prudently during its successive mating with virgin females to maximize its own fitness and the fitness of the female with whom it mated, both being most important to sericulture industry. So, the present study was undertaken and the results revealed fresh and virgin females always prefer to mate with fresh virgin males (84%) and receives high dose of ejaculates leading to higher hatchability than to virgin males of one day old (13.7%) and one day old males with mating experience (2.3%). The ejaculation size (as referred to eupyrene sperm count in the ejaculation) declined significantly over successive mating (r = -0.9931, P < 0.001), so also the male body weight (r = -0.9560, P < 0.001). The quantity of ejaculate passed to female also dramatically declined during aging (r = -0.9982, P < 0.001). It was found that male weight contribute substantially to the quantum of ejaculate (r = -0.9519, P < 0.001), so also higher fecund females receive relatively more ejaculate than the lower group to reach higher reproductive fitness. The life time fecundity was found to be $334{\pm}31$.

Assessment of genetic diversity in androgenic-based doubled haploid-derived improved restorer lines of indica rice

Bhuyan Sudhansu Sekhar,Barik Durga Prasad,Dash Byomkesh,Rout Prachitara,Pattnaik Snigdha Samir,Verma Ramlakhan,Katara Jawahar Lal,Parameswaran C.,Devanna B. N.,Sahoo Raj Kishore,Mishra Anindita,Sabari 한국작물학회 2024 Journal of crop science and biotechnology Vol.27 No.2

Hybrid rice has a considerable yield advantage over inbred lines, but the adoption rate in India is quite slow. In a three-line hybrid rice breeding programme, the development of fertility restorer lines that have enhanced grain quality is crucial. Employment of doubled haploid (DH) technology will accelerate the development of improved R lines in a short period of time, thus hastening the pace of hybrid rice breeding. This investigation was carried out for the evaluation of genetic diversity and assessment of population differentiation in 113 DHs derived from BC1F1s of IMP. IR 42266-29-3R and Nagina 22. A total of 46 SSR (Simple Sequence Repeat) markers resulted in an average of 2.00 alleles per locus. Polymorphism information content (PIC) value ranged from 0.31 to 0.37 with an average of 0.36. RM496, RM25520, RM483 and RM553 were found to be the best markers for the identification of genetic diversity. Structure analysis classified 113 DHs into four groups, which matched the Neighbour-Joining method using UPGMA cluster analysis. The AMOVA results demonstrated substantial genetic variations within subpopulation than among sub-population. The SSR marker-based molecular fingerprinting could serve as a sound basis in the identification of genetically distant as well as in the duplicate sorting of the morphologically close population in future breeding programmes

Determination of many-electron basis functions for a quantum Hall ground state using Schur polynomials

Mandal, Sudhansu S.,Mukherjee, Sutirtha,Ray, Koushik Elsevier 2018 Annals of physics Vol.390 No.-

<P><B>Abstract</B></P> <P>A method for determining the ground state of a planar interacting many-electron system in a magnetic field perpendicular to the plane is described. The ground state wave-function is expressed as a linear combination of a set of basis functions. Given only the flux and the number of electrons describing an incompressible state, we use the combinatorics of partitioning the flux among the electrons to derive the basis wave-functions as linear combinations of Schur polynomials. The procedure ensures that the basis wave-functions form representations of the angular momentum algebra. We exemplify the method by deriving the basis functions for the 5 2 quantum Hall state with a few particles. We find that one of the basis functions is precisely the Moore–Read Pfaffian wave function.</P>