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RISS 인기검색어
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- 저자 : Subramaniyam Suresh (검색결과 2 건)
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Subramaniyam Suresh,Kuravappullam Vedhaiyan Radha 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2
Mustard oilcake was used as a novel substrate for phytase production with Rhizopus oligosporus MTCC 556. A mixed substrate (wheat bran:mustard oil cake) was used to optimize different medium components under solid state fermentation at a 62% moisture content, pH 5.5, 30ºC, an inoculum level of 15%, and a ratio of the volume of a salt solution to the mass of mixed substrate 1.5 mL:10 g. Different carbon and nitrogen sources were used for production of phytase. Use of the nutritional supplements maltose and ammonium sulfate resulted in high phytase yields. The hydrolytic ability of partially purified phytase was evaluated using 5 different food grains. Wheat flour showed a 43.78% phytic acid reduction, rice flour showed 92% release of inorganic phosphorus, and corn flour showed 81 and 68% releases of soluble proteins and reducing sugars. Thus, phytase is suitable for feed applications.
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Ramasamy Selvam,Marimuthu Saravanakumar,Subramaniyam Suresh,CV Chandrasekeran,D Souza Prashanth 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.11
Objective: The study was designed to establish choline deficiency model (CDM) in broilers for evaluating efficacy of polyherbal formulation (PHF) in comparison with synthetic choline chloride (SCC). Methods: A total of 2,550 one-day-old Cobb 430 broiler chicks were randomly assigned to different groups in three experiments. In experiment 1, G1 and G2 served as normal controls and were fed a basal diet with 100% soybean meal (SBM) as a major protein source supplemented with and without SCC, respectively. In G3, G4, G5, and G6 groups, SBM was replaced at 25%, 50%, 75%, and 100% by soy protein isolate (SPI) to induce a graded level of choline deficiency. In experiment 2, PHF (500 and 1,000 g/ton) in comparison with SCC (1,000 g/ton) were evaluated. In experiment 3, dose-response of PHF (200, 400, and 500 g/ton) with SCC (400 g/ton) was determined. Results: Replacement of SBM by SPI produced a linear decrease in body weight gain (BWG) with a poor feed conversion ratio (FCR). 25% SBM replacement by SPI yielded an optimum negative impact on BWG and FCR; hence, it is considered for further studies. In experiment 2, PHF (500 and 1,000 g/ton) and SCC (1,000 g/ton) showed a similar performance in BWG, FCR and relative liver weight. In experiment 3, PHF produced an optimum efficacy at 400 g/ton and was comparable to SCC in the restoration of serum aspartate aminotransferase activity, abdominal fat, breast muscle lipid content and liver histopathological abnormalities. Conclusion: Replacement of SBM by SPI caused choline deficiency characterised by worsening of BWG, FCR, elevation in liver enzymes and histopathological changes indicating fatty liver. CDM was found valid for evaluating SCC and PHF. It is concluded that PHF has the potential to mimic biological activities of SCC through the restoration of negative effects caused by CDM.
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