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Jung, Suk-Chae,Smith, Chris L.,Lee, Ki-Sung,Hong, Min-Eui,Kweon, Dae-Hyuk,Stephanopoulos, Gregory,Jin, Yong-Su American Society for Microbiology 2010 Applied and environmental microbiology Vol.76 No.18
<B>ABSTRACT</B><P>A point mutation (E115K) resulting in slower growth of <I>E</I><I>scherichia</I><I>coli</I> DH5α and XL1-Blue in minimal media was identified in the <I>purB</I> gene, coding for adenylosuccinate lyase (ASL), through complementation with an <I>E. coli</I> K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation to the wild type restored growth phenotypes in minimal media.</P>