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- 저자 : Srisa-Art, Monpichar (검색결과 1 건)
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Analysis of Protein–Protein Interactions by Using Droplet-Based Microfluidics
Srisa-Art, Monpichar,Kang, Dong-Ku,Hong, Jongin,Park, Hyun,Leatherbarrow, Robin J.,Edel, Joshua B.,Chang, Soo-Ik,deMello, Andrew J. WILEY-VCH Verlag 2009 Chembiochem Vol.10 No.10
<P>Every little drop: The K<SUB>D</SUB> values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein–protein interactions. <img src='wiley_img/14394227-2009-10-10-CBIC200800841-content.gif' alt='wiley_img/14394227-2009-10-10-CBIC200800841-content'> </P> <B>Graphic Abstract</B> <P>Every little drop: The K<SUB>D</SUB> values of angiogenin (ANG) interactions as shown by FRET analysis of thousands of pL-sized droplets agree with data from bulk-fluorescence polarization measurements. Importantly, the use of fluorophores does not affect the activity of ANG or the binding of anti-ANG antibodies to ANG. Such an experimental platform could be applied to the high-throughput analysis of protein–protein interactions. <img src='wiley_img/14394227-2009-10-10-CBIC200800841-content.gif' alt='wiley_img/14394227-2009-10-10-CBIC200800841-content'> </P>
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