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      • KCI등재

        Nutritional alterations and damages to stored chickpea in relation with the pest status of Callosobruchus maculatus (Chrysomelidae)

        Soumaya Haouel Hamdi,Sourour Abidi,Dorra Sfayhi,Mohamed Zied Dhraief,Moez Amri,Emna Boushih,Mariam Hedjal-Chebheb,Khouja Mouhamed Larbig,Jouda Mediouni Ben Jemâa 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

        The identification of substitute products for the replacement of wheat flours for paste and bread is a strategy which may help to overcome the wheat yield problems and to meet the huge consumption rates in Tunisia. In this respect, chickpea flour can provide good opportunities. Nevertheless, seed beetles were the major constraints to achieve this goal. This paper presents extensive data on the pest status of Callosobruchus maculatus infesting chickpea in relation to its damage on chickpea seeds and flour. Data on population dynamics, growth and demographic parameters, economic injury level (EIL) and impact on functional and physical properties under two food supply conditions were studied. Results revealed that C. maculatus is a major pest altering nutritional properties of stored chickpea. Our results showed that the food supplies influence the reproductive behavior and demographic traits of C. maculatus leading to significant impacts on seed germination, weight loss and functional and physical properties. Under food optimal conditions, fertility rates were 38.1 and 47.2% respectively for Amdoun 1 and Beja 1. Moreover, the intrinsic rate of increase r and the finite rate of increase λ reached, respectively, 0.057 and 1.06 ♀/♀/days for Amdoun 1 and 0.048 and 1.05 ♀/♀/days for Beja 1. On the other hand, results showed that germination reduction depended on C. maculatus infestation level. Moreover, this work pointed out the variability of EILs with host varieties. Results also revealed that C. maculatus seed infestation led to nutritional changes in the seeds. The proximate seed composition was significantly influenced by variety and seeds category. Increases in percentages of protein (33.05 and 22.53% for 53 Amdoun 1), moisture (10.80 and 10.67% for Amdoun 1) and ash have been observed in infested seeds; decreases were observed in percentages of crude fat, carbohydrates (47.96 and 58.69% for Amdoun 1) and nutritional values (355.90 and 367.51 kcal for Amdoun 1) for the same infested seeds.

      • KCI등재

        Screening for insecticidal potential and acetylcholinesterase activity inhibition of Urginea maritima bulbs extract for the control of Sitophilus oryzae (L.)

        Asma Mami Maazoun,Takoua Ben Hlel,Soumaya Haouel Hamdi,Feten Belhadj,Jouda Mediouni Ben Jemâa,Mohamed Nejib Marzouki 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3

        Interest in botanical insecticides derived from plants has increased as a result of environmental concerns and insect resistance to chemical insecticides. In this study, we explored the insecticidal activity of Urginea maritima bulbs extract and its inhibitory effect on acetylcholinesterase enzyme system in the rice weevil Sitophilus oryzae (L.) (Coleoptera: Curculionidae). Phytochemical screening and HPLC-ESI/TOF-MS analysis revealed the presence of bioactive molecules with high insecticidal potential. Content of polyphenols (130.88 ± 0.44 mg GAE/g FW), flavonoids (50.81 ± 0.25 mg RE/g FW) as well as alkaloids (12.09 ± 0.16 mg AE/g FW) were high. HPLCESI/ TOF-MS analysis results demonstrated that ferulic acid (124.19 μg/g FW), vanillic acid (75.84 μg/g FW) and 4-hydroxybenzoic acid (59.73 μg/g FW) were the major phenolic compounds of U. maritima bulbs extract. The toxic potential against the rice weevil using contact and repellent bioassays showed the highest insecticidal potential of U. maritima bulbs extract. The LD50, LC50 and RC50 values were 19.03 μg/insect, 2.35 μg/cm2 and 0.009 μg/cm2 for contact bioassay by topical application, contact bioassay by treated filter paper method and repellent bioassay, respectively. Furthermore, U. maritima bulbs extract inhibited acetylcholinesterase (AChE) activity and median inhibition concentration IC50 was evaluated to 66.08 μg/ml. The findings of the present investigation confirmed that Urginea maritima may be recommended as an eco-friendly alternative to synthetic insecticides.

      • KCI등재

        Parasitoids of chickpea leafminer Liriomyza cicerina (Diptera: Agromyzidae) and their parasitism rate on chickpea fields in North Tunisia

        Abir Soltani,Ahmet Beyareslan,Lütfiye Gençer,Soumaya Haouel Hamdi,Arbia Bousselmi,Moez Amri,Jouda Mediouni Ben Jemâa 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4

        In recent years, as a consequence of climatic changes, the leafminer Liriomyza cicerina had emerged as a serious pest in chickpea crops causing considerable damage in Tunisia. Thus, the search of control methods is necessary. The aim of this research was to monitor and identify the naturally occurring parasitoids of the chickpea leafminer Liriomyza cicerina in insecticide-free chickpea-growing areas located in North-Western Tunisia. Chickpea leaves with mines were weekly sampled from Beja and Kef sites during 2016 and 2017 and kept to observe and count emerging leafminer and parasitoid adults in the laboratory. Results revealed that L. cicerina parasitoids complex species included three hymenopteran parasitoids namely Opius monilicornis (Fisher, 1962) (Braconidae); Diglyphus isaea (Walker, 1838) (Eulophidae) and Diaulinopsis arenaria (Erdös, 1951) (Eulophidae). These parasitoids were identified and recorded for the first time in Tunisia on L. cicerina with parasitism rate varied between 13% and 52.5%. The most common species was O. monilicornis. The correlation between infestation rate on chickpea plants and number of emerged three parasitoids was calculated revealing high significant and positive correlations. The diversity index (H′) was also calculated. The three parasitoids were found in two sites Beja and Kef; while the diversity differed during the developmental stage of chickpea crops.

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