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A facile approach to enhance antigen response for personalized cancer vaccination
Li, Aileen Weiwei,Sobral, Miguel C.,Badrinath, Soumya,Choi, Youngjin,Graveline, Amanda,Stafford, Alexander G.,Weaver, James C.,Dellacherie, Maxence O.,Shih, Ting-Yu,Ali, Omar A.,Kim, Jaeyun,Wucherpfen Nature Publishing Group UK 2018 Nature materials Vol.17 No.6
<P>Existing strategies to enhance peptide immunogenicity for cancer vaccination generally require direct peptide alteration, which, beyond practical issues, may impact peptide presentation and result in vaccine variability. Here, we report a simple adsorption approach using polyethyleneimine (PEI) in a mesoporous silica microrod (MSR) vaccine to enhance antigen immunogenicity. The MSR-PEI vaccine significantly enhanced host dendritic cell activation and T-cell response over the existing MSR vaccine and bolus vaccine formulations. Impressively, a single injection of the MSR-PEI vaccine using an E7 peptide completely eradicated large, established TC-1 tumours in about 80% of mice and generated immunological memory. When immunized with a pool of B16F10 or CT26 neoantigens, the MSR-PEI vaccine eradicated established lung metastases, controlled tumour growth and synergized with anti-CTLA4 therapy. Our findings from three independent tumour models suggest that the MSR-PEI vaccine approach may serve as a facile and powerful multi-antigen platform to enable robust personalized cancer vaccination.</P>
Sn loaded Au–ZnO photocatalyst for the degradation of AR 18 dye under UV-A light
A. Senthilraja,B. Krishnakumar,B. Subash,Abilio J.F.N. Sobral,M. Swaminathan,M. Shanthi 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.33 No.-
Sn–Au–ZnO was successfully synthesized by precipitation–decomposition method. The catalyst wascharacterized by XRD, FE-SEM, EDS, TEM, DRS, PL, XPS, TGA, DTA and BET surface area measurements. Sn–Au–ZnO is found to be more efficient than bare ZnO and other commercial catalysts at pH 11 for themineralization of AR 18 dye under UV-A light. The effects of operational parameters on photomineralization of AR 18 dye have been investigated. The mineralization of dye has been confirmed byCOD measurements. A possible mechanism is proposed for the degradation of AR 18 under UV-A light. The catalyst is found to be reusable
Effect of ripening time on bacteriological and physicochemical goat milk cheese characteristics
Rodrigo V. Moreira,Marion P. Costa,Beatriz S. Frasao,Vivian S. Sobral,Claudius C. Cabral,Bruna L. Rodrigues,Se´rgio B. Mano,Carlos A. Conte-Junior 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.4
Cheese ripening involves lactose metabolism, lipolysis and proteolysis, which are affected by many factors. The aim of this study was to assess changes due to ripening (90 days) of goat milk cheese through bacteriological and physicochemical analysis in order to verify if, at the end of ripening period, this cheese could be considered ‘‘lactose-free’’. Three batches of the goat milk cheese were manufactured and ripened at 10 C and 80% relative humidity for 90 days. Titratable acidity increased by about 59 D due to carbohydrate degradation and organic acid production. However, pH (5.31–5.25) remained constant. Lactococcus was the dominant cheese microbiota, acting in the fermentation of lactose (1.17–0.06 mg/g) and lactic acid production (5.49– s10.01 mg/g). Thus, ripening time was decisive for bacteriological and physicochemical goat milk cheese characteristics.
Kumar, Santosh,Garg, Pankaj,Pandey, Shambhavi,Kumari, Mridula,Hoon, Seonwoo,Jang, Kyoung-Je,Kapavarapu, Ravikumar,Choung, Pill-Hoon,Sobral, Abilio J. F. N.,Hoon Chung, Jong The Royal Society of Chemistry 2015 Journal of Materials Chemistry B Vol.3 No.17
<P>Gene therapy is the treatment of human disorders by the introduction of genetic material to specific target cells of a patient. Chitosan and its derivatives show excellent biological properties including biocompatibility, biodegradability and nonallergenicity. Primary amines of chitosan are responsible for its cationic nature and hence binding and protection of DNA for intracellular delivery. But the transfection efficiency of chitosan based gene transporters is severely hampered by its poor physical properties such as low water solubility and high viscosity. In this study, primary amines of low molecular weight (LMW) chitosan were coupled with 2-acrylamido-2-methylpropane sulphonic acid (AMP) making it water soluble for its application in gene delivery. AMP modified chitosan (CSAMP) showed an enhanced interaction with DNA and a higher buffering capacity due to AMP amines leading to a higher transfection efficiency in cancer cells (A549, HeLa and HepG2) compared to native chitosan and Lipofectamine®. <I>In vivo</I> studies in Balb/c through intravenous injection demonstrated a higher luciferase expression compared to LMW chitosan.</P>