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RISS 인기검색어
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- 저자 : Smooker, A M (검색결과 2 건)
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Smooker, A M,Wells, R,Morgan, C,Beaudoin, F,Cho, K,Fraser, F,Bancroft, I Springer 2011 TAG. Theoretical and applied genetics. Theoretisch Vol.122 No.6
<P>We constructed a linkage map for the population QDH, which was derived from a cross between an oilseed rape cultivar and a resynthesised Brassica napus. The linkage map included ten markers linked to loci orthologous to those encoding fatty acid biosynthesis genes in Arabidopsis thaliana. The QDH population contains a high level of allelic variation, particularly in the C genome. We conducted quantitative trait locus (QTL) analyses, using field data obtained over 3 years, for the fatty acid composition of seed oil. The population segregates for the two major loci controlling erucic acid content, on linkage groups A8 and C3, which quantitatively affect the content of other fatty acids and is a problem generally encountered when crossing 'wild' germplasm with cultivated 'double low' oilseed rape cultivars. We assessed three methods for QTL analysis, interval mapping, multiple QTL mapping and single marker regression analysis of the subset of lines with low erucic acid. We found the third of these methods to be most appropriate for our main purpose, which was the study of the genetic control of the desaturation of 18-carbon fatty acids. This method enabled us to decouple the effect of the segregation of the erucic acid-controlling loci and identify 34 QTL for fatty acid content of seed oil, 14 in the A genome and 20 in the C genome. The QTL indicate the presence of 13 loci with novel alleles inherited from the progenitors of the resynthesised B. napus that might be useful for modulating the content or extent of desaturation of polyunsaturated fatty acids, only one of which coincides with the anticipated position of a candidate gene, an orthologue of FAD2.</P>
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Cho, Kwangsoo,O’Neill, Carmel M.,Kwon, Soo-Jin,Yang, Tae-Jin,Smooker, Andrew M.,Fraser, Fiona,Bancroft, Ian Blackwell Publishing Ltd 2010 The Plant journal Vol.61 No.4
<P>Summary</P><P>We conducted a sequence-level comparative analyses, at the scale of complete bacterial artificial chromosome (BAC) clones, between the genome of the most economically important <I>Brassica</I> species, <I>Brassica napus</I> (oilseed rape), and those of <I>Brassica rapa</I>, the genome of which is currently being sequenced, and <I>Arabidopsis thaliana</I>. We constructed a new <I>B. napus</I> BAC library and identified and sequenced clones that contain homoeologous regions of the genome including stearoyl-ACP desaturase-encoding genes. We sequenced the orthologous region of the genome of <I>B. rapa</I> and conducted comparative analyses between the <I>Brassica</I> sequences and those of the orthologous region of the genome of <I>A. thaliana</I>. The proportion of genes conserved (∼56%) is lower than has been reported previously between <I>A. thaliana</I> and <I>Brassica</I> (∼66%). The gene models for sets of conserved genes were used to determine the extent of nucleotide conservation of coding regions. This was found to be 84.2 ± 3.9% and 85.8 ± 3.7% between the <I>B. napus</I> A and C genomes, respectively, and that of <I>A. thaliana</I>, which is consistent with previous results for other <I>Brassica</I> species, and 97.5 ± 3.1% between the <I>B. napus</I> A genome and <I>B. rapa</I>, and 93.1 ± 4.9% between the <I>B. napus</I> C genome and <I>B. rapa</I>. The divergence of the <I>B. napus</I> genes from the A genome and the <I>B. rapa</I> genes was greater than anticipated and indicates that the A genome ancestor of the <I>B. napus</I> cultivar studied was relatively distantly related to the cultivar of <I>B. rapa</I> selected for genome sequencing.</P>
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