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Samira Jebahi,Hassane Oudadesse,Jiheun Elleuch,Slim Tounsi,Hassib Keskese,Pascal Pellen-Mussi,Tarek Rebai,Abdelfatteh El Feki,Hafed El Feki 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.5
Strontium (Sr) compounds have become increasingly popular in the field of osteoporosis treatment. However, the quality of new bone after implantation of strontium-containing bioceramics has yet to be investigated. In the present study, the newly formed bone tissue around strontium-doped bioactive glass (BG-Sr) implants was characterized. BG-Sr was implanted in the femoral condyl of ovariectomised rats (OVX). The resected bone was prepared for analysis using several physico-chemical and biological assays such as Fourier transform infrared spectroscopy,X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray, and histomorphometry. BG-Sr biomaterial favored calcium phosphate layer integration on the surface of the glass and offered better bioactivity. Moreover, the histomorphometric analysis demonstrated that BV/TV, N. Ob were significantly higher in BGSr treated rats groups than those of BG groups. However, Ob. S/BS, and OV/BV were significantly lower in BG-Sr treated rats groups than those of BG groups. The (Oc.S/ BS) was significantly decreased in BG-Sr groups, when compared with that of BG rat groups. On the other hand, the MS/BS had not significantly decreased in the BG-Sr treated rats groups when compared with that of BG groups, however; it was significantly higher when compared with control and OVX groups. These findings suggest that BG-Sr can be used as an inhibitory therapeutic potential of osteoporosis by delivering strontium to stimulate new bone remodeling.
Jebahi, Samira,Oudadesse, Hassane,Elleuch, Jiheun,Tounsi, Slim,Keskes, Hassib,Pellen, Pascal,Rebai, Tarek,El Feki, Abdelfatteh,El Feki, Hafed The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6
Strontium (Sr) compounds have become increasingly popular in the field of osteoporosis treatment. However, the quality of new bone after implantation of strontium-containing bioceramics has yet to be investigated. In the present study, the newly formed bone tissue around strontium-doped bioactive glass (BG-Sr) implants was characterized. BG-Sr was implanted in the femoral condyl of ovariectomised rats (OVX). The resected bone was prepared for analysis using several physico-chemical and biological assays such as Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray, and histomorphometry. BG-Sr biomaterial favored calcium phosphate layer integration on the surface of the glass and offered better bioactivity. Moreover, the histomorphometric analysis demonstrated that BV/TV, N. Ob were significantly higher in BG-Sr treated rats groups than those of BG groups. However, Ob. S/BS, and OV/BV were significantly lower in BG-Sr treated rats groups than those of BG groups. The (Oc.S/BS) was significantly decreased in BG-Sr groups, when compared with that of BG rat groups. On the other hand, the MS/BS had not significantly decreased in the BG-Sr treated rats groups when compared with that of BG groups, however; it was significantly higher when compared with control and OVX groups. These findings suggest that BG-Sr can be used as an inhibitory therapeutic potential of osteoporosis by delivering strontium to stimulate new bone remodeling.
( Kais Jamoussi ),( Sameh Sellami ),( Zina Nasfi ),( Saloua Krichen Makni ),( Slim Tounsi ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Tuta absoluta (Povolny, 1994) is a devastating moth to the Solanaceae plants. It is a challenging pest to control, especially on tomatoes. In this work, we studied the entomopathogenic activity of the Cry-forming δ-endotoxins produced by Bacillus thuringiensis strain KS and B. thuringiensis kurstaki reference strain HD1 against T. absoluta. These strains carried the cry2, cry1Ab, cry1Aa / cry1Ac, and cry1I genes, and KS also carried a cry1C gene. The δ-endotoxins of KS were approximately twofold more toxic against the third instar larvae than those of HD1, as they showed lower 50% and 90% lethal concentrations (0.80 and 2.70 μg/cm² (δ-endotoxins/tomato leaf)) compared with those of HD1 (1.70 and 4.50 μg/cm²) (p < 0.05). Additionally, the larvae protease extract showed at least six caseinolytic activities, which activated the KS and HD1 δ- endotoxins, yielding the active toxins of about 65 kDa and the protease-resistant core of about 58 kDa. Moreover, the histopathological effects of KS and HD1 δ-endotoxins on the larvae midgut consisted of an apical columnar cell vacuolization, microvillus damage, and epithelial cell disruption. These results showed that the KS strain could be a candidate for T. absoluta control.
Salha Boulila,Hafed Elfeki,Hassane Oudadesse,Rim Kallel,Bertrand Lefeuvre,Mostafa Mabrouk,Slim Tounsi,Dhekra Mhalla,Amany Mostafa,Khansa Chaabouni,Fatma Makni-Ayedi,Allal Barroug,Tahia Boudawara,Abdel 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.5
Bioactive glass particle is used in the repair of bone defects. This material undergoes a series of surface in vivo reactions, which leads to osteointegration. We evaluated the effect of the bioactive glass synthesis, sol-gel (BG(S)) versus melting (BG(M)), associated with polyvinyl-alcohol (PVA) on in vivo bioactivity with biochemical parameters, liver-kidney histological structure and antibacterial in vitro activity. These composites were testified in many bacteria and implanted in ovariectomized rat. The serum and organs (liver and kidney) of all groups, control and treated rats, were collected to investigate the side effects of our composites, BG(S)-PVA and BG(M)-PVA, in comparison with control and ovariectomized rats. Also, the implants, before and after implantation, were prepared for analysis using physicochemical techniques such as Fourier transform infrared spectroscopy and X-ray diffraction. Our results have shown the stability of natremia, kaliemia, calcemia and phosphoremia. The histological structures of liver and kidney in implanted rats are intact compared to control and ovariectomized rats. BG(S)-PVA is characterized by a higher antibacterial effect on negative and positive gram bacteria than BG(M)-PVA. The physicochemical results have confirmed a progressive degradation of BG(S)-PVA and BG(M)-PVA, while replacing the implant by an apatite layer. But this bioactivity of BG(S)-PVA is faster than BG(M)-PVA. We can therefore confirm, on the one hand, the biocompatibility of our two implants and, on the other hand, the beneficial effect of sol-gel synthesis technique versus melting, both on the antibacterial effect and on the rapid formation of layer hydroxyapatite, and consequently on osteogenesis.