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Wacim Bejar,Ameny Farhat-Khemakhem,Slim Smaoui,Mohamed Makni,Mounira Ben Farhat,Badis Abdelmalek,Lotfi Mellouli,Samir Bejar,Hichem Chouayekh,Emmanuelle Maguin 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.6
Nine lactobacilli previously selected for high antagonism against food borne bacterial pathogens were identified via 16S rRNA gene sequencing and screened for probiotic potential for use in poultry production. The lactobacilli were subjected to a subtractive in vitro analysis system using a certified probiotic as reference. This allowed for selection of a milk-derived Lactobacillus plantarum strain, termed TN627. This organic acid-producing bacterium was free of harmful enzymatic activity and sensitive to several antibiotics. It also showed good growth at pH 4 and in the presence of bile. L. plantarum TN627 also exhibited high efficacy of adhesion to chicken enterocytes, which correlated with detecting genes encoding the mucusbinding,adhesion-promoting proteins (Mub and MapA)and the adhesion-like factor EF-Tu, commonly involved in adherence of lactobacilli to mucosal surfaces. Taken together, our findings suggest that TN627 is a promising probiotic candidate with high potential for application as a supplement in the animal feed industry.
Felhi, Samir,Baccouch, Noura,Salah, Hichem Ben,Smaoui, Slim,Allouche, Noureddine,Gharsallah, Neji,Kadri, Adel 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The present study revealed that the nutritive value of grape seeds (Vitis vinifera L.) was $383.55{\pm}0.13Kcal/100g$, with magnesium as the most abundant mineral element ($70.44{\pm}0.88 mg/L$). The maximum phenolic ($392.58{\pm}1.70mg\;of\;GAE/g$), flavonoid ($256.16{\pm}1.60mg\;of\;QE/g$), and tannin ($30.95{\pm}0.17mg\;of\;CE/g$) contents were also found in the ethanol, dichloromethane, and hexane extracts, respectively. The major phytochemical compounds in the ethyl acetate extract were identified via gas chromatography-mass spectrometry (GC-MS) analysis. The ethanol extract has the highest antioxidant activity ($IC_{50}=140{\pm}1.20{\mu}g/mL$ for DPPH, $145.28{\pm}0.45mg$ ${\alpha}-tocopherol/g$ for total antioxidant capacity, and $EC_{50}=80{\pm}1.41{\mu}g/mL$ for ferric-reducing power assays). For ${\beta}-carotene$ test, the highest antioxidant activity was obtained in the hexane extract. A satisfactory antimicrobial activity was found against a panel of microorganisms with the ethyl acetate extract as the best antimicrobial agent. Additionally, it was found that the bactericidal concentration required for the grape seed extract to kill Listeria monocytogenes should be less than 12.50 mg/mL (minimum inhibitory concentration=4).
Samir Felhi,Noura Baccouch,Hichem Ben Salah,Slim Smaoui,Noureddine Allouche,Néji Gharsallah,Adel Kadri 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The present study revealed that the nutritive value of grape seeds (Vitis vinifera L.) was 383.55±0.13 Kcal/100 g, with magnesium as the most abundant mineral element (70.44±0.88 mg/L). The maximum phenolic (392.58±1.70mg of GAE/g), flavonoid (256.16±1.60 mg of QE/g), and tannin (30.95±0.17mg of CE/g) contents were also found in the ethanol, dichloromethane, and hexane extracts, respectively. The major phytochemical compounds in the ethyl acetate extract were identified via gas chromatography–mass spectrometry (GC–MS) analysis. The ethanol extract has the highest antioxidant activity (IC50=140±1.20 μg/mL for DPPH, 145.28±0.45mg α-tocopherol/g for total antioxidant capacity, and EC50=80±1.41 μg/mL for ferric-reducing power assays). For β-carotene test, the highest antioxidant activity was obtained in the hexane extract. A satisfactory antimicrobial activity was found against a panel of microorganisms with the ethyl acetate extract as the best antimicrobial agent. Additionally, it was found that the bactericidal concentration required for the grape seed extract to kill Listeria monocytogenes should be less than 12.50 mg/mL (minimum inhibitory concentration=4).