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        A Novel Recombined Potato virus Y Isolate in China

        Shuxin Han,Yanling Gao,Guoquan Fan,Wei Zhang,Cailing Qiu,Shu Zhang,Yanju Bai,Junhua Zhang,Carl Spetz 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.4

        This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants (PVYN-Wi) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other PVYN-Wi isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical PVYN-Wi isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.

      • SCIEKCI등재

        A Novel Recombined Potato virus Y Isolate in China

        Han, Shuxin,Gao, Yanling,Fan, Guoquan,Zhang, Wei,Qiu, Cailing,Zhang, Shu,Bai, Yanju,Zhang, Junhua,Spetz, Carl The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.4

        This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants ($PVY^{N-Wi}$) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other $PVY^{N-Wi}$ isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical $PVY^{N-Wi}$ isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.

      • KCI등재

        Dynamic Response of High Strength Steel Beams Subjected to Explosion Induced Blast Load

        Xinchang Feng,Xiyue Liu,Zhiyang He,Shuxin Bai,Shun Li,Yu Tang 한국강구조학회 2023 International Journal of Steel Structures Vol.23 No.6

        A series of anti-explosion tests were performed on H-type high strength steels (Q460JSC and HQ600) and ordinary steels (Q345B) to address the problem of anti-explosion in the technical application of high strength steel. The dynamic behavior of steel beams during explosion and the propagation of shock waves were analyzed. The effects of proportionate distance, steel strength, high-span ratio, section shapes were investigated. The finite element software (ANSYS/LS-DYNA) was used to analyze the anti-explosion performance of high strength steel beams. The finite element models which adopted modified Johnson–Cook constitutive model and damage criterion were validated by comparing with the experimental results. The influence factors of anti-explosion performance of high strength steel were explored by numerical simulation as well. The results reveal that under near-explosion conditions, the peak values of overpressure predicted by empirical formulas are often less than the experimental outcomes. As the scaled distance reduces, the damage to the steel beam increases, making it more prone to local fracture. By decreasing the scaled distance, increasing the high-span ratio, and strengthening the constraint of H-type steel beam, the deformation and damage of steel beams can reduce. The results provide an effective basis to evaluate the safety of high strength steel beams applied in practical engineering when subjected to explosion.

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