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Debjani Ghosh,Shruti S. Bandyopadhyay,Udipta R Chatterjee,Bimalendu Ray,Peter Capek 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2
Chirata (Swertia chirata) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we analyzed the water-extracted carbohydrate polymer (WECP) of this herb using chemical, chromatographic,and spectroscopic methods. The anti-oxidant capacity of this fraction was studied by ferric reducing anti-oxidant power (FRAP) and DPPH radical assays. Effect of WECP on bovine serum albumin spectrum (BSA) was determined using excitation wavelength of 282 nm. Anion exchange chromatography of WECP yielded 5 fractions (F1-F5) with different chemical compositions. The major fraction (F5)was homogeneous, had an apparent molecular mass of 4.5kDa, and contains both carbohydrates (57%) and phenolics (34%). The anti-oxidant capacities of WECP and F5 were comparable to standard anti-oxidants. Notably, activities of the carbohydrate polymers (F1-F5) correlate with their phenolics content. Fluorescence quenching measurement suggests that F5 can form complex with BSA and the value of the binding constant is K=6.28×105/M.
Sharmistha Sinha,Shruti S. Bandyopadhyay,Debjani Ghosh,Udipta Ranjan Chatterjee,Sudipta Saha,Pradyot K. Ghosal,Bimalendu Ray 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.4
Senna (Cassia angustifolia) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we have demonstrated that water extracted arabinogalactan protein (AGP) rich fraction possesses strong antioxidative activity. The AGP rich fraction was analyzed using chemical, chromatographic, and spectroscopic methods. Effect of water extracted polymers on bovine serum albumin spectrum was determined using excitation wavelength of 282 nm. The antioxidant capacity of this fraction was studied by ferric reducing antioxidant power (FRAP) and DPPH radical assays. This polymeric fraction,≥88% of which gets precipitated with Yariv reagent,consisted mainly of (1→5)-/(1→3,5)-linked α-arabinosyl,(1→3)-/(1→3,6)-linked-galactosyl residues and terminal arabinofuranosyl residues. Its in vitro antioxidant capacity is comparable to that of standard antioxidants. Fluorescence quenching studies furnishes evidence for the interaction of the arabinogalactan protein with bovine serum albumin.