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Immobilization of cellulase on functionalized cobalt ferrite nanoparticles
Shivaji Hariba Pawar,Raghvendra Ashok Bohara,Nanasaheb Devappa Thorat 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.1
Amine functionalized cobalt ferrite (AF-CoFe2O4) magnetic nanoparticles (MNPs) were used for immobilization of cellulase enzyme via 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDS) and N-hydroxysuccinimide (NHS) coupling reaction. The structural, morphological and magnetic properties of AF-CoFe2O4 were determined. TEM micrograph revealed a mean diameter of ~8 nm and showed that the AF-CoFe2O4 remain distinct with no significant change in size after binding with cellulase. Fourier transform infrared (FT-IR) spectroscopy confirmed the binding of cellulase to AF-CoFe2O4. The properties of immobilized cellulase were investigated by optimizing binding efficiency, pH, temperature and reusability. The results showed that the immobilized cellulase has higher thermal stability than free cellulase, which might be due to covalent interaction between cellulase and AF-CoFe2O4 surface. The immobilized cellulase also showed good reusability after recovery. Therefore, AF-CoFe2O4 MNPs can be considered as promising candidate for enzyme immobilization.
Developments in photocatalytic antibacterial activity of nano TiO2: A review
Hemraj Mahipati Yadav,Shivaji Hariba Pawar,Jung-Sik Kim 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.7
TiO2, which is one of the most explored materials, has emerged as an excellent photocatalyst material for environmental and energy fields, including air and water purification, self-cleaning surfaces, antibacterial and water splitting. This review summarizes recent research developments of TiO2-based photocatalyst used for photocatalytic antibacterial applications. Several strategies to enhance the efficiency of TiO2 photocatalyst are discussed, including doping with metal ions, noble metals, non-metals, and coupling with other materials. The mechanism of photocatalytic antibacterial activity in the presence of nano-sized TiO2 is also discussed. The modified TiO2 photocatalyst significantly inhibits the growth of bacterial cells in response to visible light illumination. TiO2 photocatalysis appears to be promising as a route of advanced oxidation process for environmental remediation.