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RISS 인기검색어
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- 저자 : Shillitoe, E.J. (검색결과 1 건)
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Shillitoe, E.J.,Hermonat, P.L.,Noonan, S.,Shi, B. Korean Academy of Oral Biology and the UCLA Dental 2001 International Journal of Oral Biology Vol.26 No.1
The p5 and p40 promoters of the human adeno-associated virus (AAV) were tested for ability to promote expression of either the lacZ marker gene or the thymidine kinase (TK) suicide gene in human oral cancer cells. For comparison, the early gene promoter of the SV40 virus and the immediate-early promoter of human cytomegalovirus (CMV) were used. In the oral cancer cell line Tu183 the highest levels of expression of the marker gene was obtained from the CMV promoter. Lower levels were seen from the SV40 promoter and lower still from the p5 and p40 promoters. Nonetheless, both p5 and p40 were sufficiently active to produce a toxic level of expression of the TK suicide gene, with the p5 promoter being extremely effective. Testing in neuroblastoma cells revealed that the p5 and p40 promoters were less cancer-specific than the other promoters. Neither adenovirus nor hydroxyurea increased the expression from any promoter. The results imply that 1) the promoters of AAV could be used in gene therapy of human squamous cell carcinomas for expression of toxic drugs or other purposes, 2) the presence of DNA-damaging agents should not be expected to enhance the effects of the promoters, and 3) no specificity for oral cancer cells may be expected.
RISS
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