Molecular cloning and characterization of novel human JNK2 (MAPK9) transcript variants that show different stimulation activities on AP-1

( Ping Zhang Wang ),( Ying Xiong ),( Chuan Ma ),( Tai Ping Shi ),( Da Long Ma ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.11

The c-Jun NH2-terminal kinase (JNK) signaling pathway participates in many physiological functions. In the current study we reported the cloning and characterization of five novel JNK2 transcript variants, which were designated as JNK2 α3, JNK2 α4, JNK2 β3, JNK2 γ1 and JNK2 γ2, respectively. Among them, JNK2 α4 and JNK2 γ2 are potential non-coding RNA because they contain pre-mature stop codons. Both JNK2α3 and JNK2β3 contain an intact kinase domain, and both encode a protein product of 46 kDa, the same as those of JNK2α1 and JNK2β1. JNK2γ1 contains a disrupted kinase domain and it showed a disable function. When over-expressed in mammalian cells, JNK2α3 showed higher activity on AP-1 than that of JNK2β3 and JNK2γ1. Furthermore, JNK2α3 and JNK2β3 showed different levels of substrate phosphorylation, although they both could promote the proliferation of 293T cells. Our results further demonstrate that JNK2 isoforms preferentially target different substrates and may regulate the expression of various target genes. [BMB reports 2010; 43(11): 738-743]

Human HS1BP3 induces cell apoptosis and activates AP-1

( Tai Ping Shi ),( Jie Shi Xie ),( Ying Xiong ),( Wei Wei Deng ),( Jin Hai Guo ),( Feng Wang ),( Da Long Ma ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.6

In the present study, we characterized the function of HS1-binding protein 3 (HS1BP3), which is mutated in essential tremor and may be involved in lymphocyte activation. We found that HS1BP3 localized to the mitochondria and endoplasmic reticulum partially. Overexpression of HS1BP3 induced apoptosis in HEK293T and HeLa cell lines. When these cell lines were transfected with HS1BP3, they exhibited nuclear DNA condensation, externalization of phosphatidylserine (PS), and cleavage of poly ADP ribose polymerase (PARP). Furthermore, suppression of HS1BP3 or HS1 expression attenuates HS1BP3 induced apoptosis. In addition, HS1BP3 enhanced activator protein 1 (AP-1)-mediated transcription in a dose-dependent manner. Therefore, we conclude that HS1BP3 regulates apoptosis via HS1 and stimulates AP-1-mediated transcription. [BMB reports 2011; 44(6): 381-386]

모션그래픽에 나타난 메타포 표현 - 솔바스와 카일쿠퍼의 영화 오프닝 타이틀을 중심으로 -

왕사평 ( Shi-ping Wang ),김해태 ( Hae-tae Kim ) 한국디자인트렌드학회 2021 한국디자인포럼 Vol.26 No.1

연구배경 모션그래픽은 정보를 재설계하고 평면, 광고전파, 애니메이션, 영화 등 많은 분야에서 융합되어 활용되어 지고 있다. 특히 영상에 표현된 메타포는 정보 전달 수단이자 인지를 간소화하는 방법이다. 메타포 이론으로 영상을 분석하면 영화를 더 잘 해석하고 본질을 발굴해 규율을 탐구하는 데 도움이 된다. 연구방법 영화 오프닝타이틀의 대표적인 디자이너 솔바스(Saul Bass)와 카일 쿠퍼(Kyle Cooper)의 작품이 분석 대상이다. 방법은 영화 오프닝 타이틀의 구성요소와 모션그래픽의 조형요소로 구분하여 메타포의 유형별 특징을 근거로 메타포 언어와 이미지 분석을 통해 영화 오프닝의 시각적 이미지에 대한 메타포 표현을 분석한다. 연구결과 영화 오프닝 타이틀은 메타포를 활용하여 다소 무거운 주제와 답답한 배경을 결합하고 고도로 감정을 파고드는 표현으로 영화 속 은유적인 요소뿐만 아니라 내면의 열광적인 예술적 특성까지 드러내며 뛰어난 예술적 상상력과 표현력을 보여주었다. 결론 영화의 오프닝 타이틀은 모션그래픽의 표현으로 감각적 자극과 무한한 상상을 관객들에게 보여주었다. 또한 영화 오프닝 타이틀의 발전과 맥락을 맞추거나 보완하면서 음악, 문자, 메타포 등 디자인과 영화언어의 조합을 통해 보여주는 새로운 시각적 표현방식을 실감케 했다. 이에 본 연구는 모션그래픽의 발달에 대한 이론적 근거를 제공할 수 있고 미래 모션그래픽의 효율적인 발전 방향을 제시하고 궁극적으로 확장된 사고를 통하여 미래 정보 확산에 영향을 미칠 수 있는 연구가 되기를 기대한다. Background Motion graphics are being redesigned and used in many areas, including planar, advertising, animation, and film. Metaphor in particular expressed in images, is a means of information delivery and a method of simplifying recognition. Analyzing images with metaphor theory helps to better interpret movies and explore discipline by discovering the essence. Methods The works of leading designers Saul Bass and Kyle Cooper in the opening title of the films are always subjected to analysis. The method breaks down the components of the film opening title and the formative elements of motion graphics to analyze the metaphor representation of the film opening through the metaphor language and image analysis based on the type-specific characteristics of the metaphor. Result The opening title of the film combines a rather heavy theme with a stuffy background using metaphor, which reveals not only the metaphorical elements in the film but also the enthusiastic artistic characteristics, and shows the imagination and expression of this outstanding artistic. Conclusion The opening title of film is an expression of motion graphics, showing the audiences’ sensory stimulation and infinite imagination. It also shows a new way of visual expression through a combination of design and film language, such as music, text, and metaphor, while matching or complementing the development of the opening title. Therefore, this work provides a theoretical basis for the development of motion graphics. We hope to present efficient development directions for future motion graphics, and ultimately influence the future information diffusion through extended thinking.

No Association Between the USP7 Gene Polymorphisms and Colorectal Cancer in the Chinese Han Population

Li, Xin,Wang, Yang,Li, Xing-Wang,Liu, Bao-Cheng,Zhao, Qing-Zhu,Li, Wei-Dong,Chen, Shi-Qing,Huang, Xiao-Ye,Yang, Feng-Ping,Wang, Quan,Wang, Jin-Fen,Xiao, Yan-Zeng,Xu, Yi-Feng,Feng, Guo-Yin,Peng, Zhi-Ha Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.5

Colorectal cancer (CRC), now the third most common cancer across the world, is known to aggregate in families. USP7 is a very important protein with an important role in regulating the p53 pathway, which is critical for genomic stability and tumor suppression. We here genotyped eight SNPs within the USP7 gene and conducted a case-control study in 312 CRC patients and 270 healthy subjects in the Chinese Han population. No significant associations were found for any single SNP and CRC risk. Our data eliminate USP7 as a potential candidate gene towards for CRC in the Han Chinese population.

Mutations of TYR and MITF Genes are Associated with Plumage Colour Phenotypes in Geese

Wang, Ye,Li, Si-Ming,Huang, Jing,Chen, Shi-Yi,Liu, Yi-Ping Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6

The polymorphism of microphthalmia-associated transcription factor (MITF) and tyrosinase (TYR) genes have been proposed to play a vital role in coat colour genesis in mammals, but their role remains ambiguous in geese at best. Here, we cloned and sequenced 1,397 bp coding region of MITF gene and a 588 bp fragment of TYR exon 1 for polymorphism analysis among 157 domestic geese showing three types of plumage colour. We detected a total of three SNPs (c.280T>C, c.345G>A, and c.369G>A) in TYR and six haplotypes (H1-H6). Among them, haplotypes H1, H2, H3, and H5 were significantly associated with white plumage trait of Zhedong White Geese. However, only diplotype H1H1 and H3H5 were significantly associated with white plumage trait of Zhedong White Geese (p<0.01). We only detected one SNP (c.1109C>T) for MITF gene and found that genotype CT and TT were significantly associated with white plumage trait of Zhedong White Geese. Briefly, our study suggested an association between polymorphisms of TYR and MITF genes and the plumage colour trait in domestic geese.

Optimized Low-Switching-Loss PWM and Neutral-Point Balance Control Strategy of Three-Level NPC Inverters

Shi-Zhou Xu,Chun-Jie Wang,Tian-Cheng Han,Xue-Ping Li,Xiang-Yu Zhu 전력전자학회 2018 JOURNAL OF POWER ELECTRONICS Vol.18 No.3

Power loss reduction and total harmonic distortion(THD) minimization are two important goals of improving three-level inverters. In this paper, an optimized pulse width modulation (PWM) strategy that can reduce switching losses and balance the neutral point with an optional THD of three-level neutral-point-clamped inverters is proposed. An analysis of the two-level discontinuous PWM (DPWM) strategy indicates that the optimal goal of the proposed PWM strategy is to reduce switching losses to a minimum without increasing the THD compared to that of traditional SVPWMs. Thus, the analysis of the two-level DPWM strategy is introduced. Through the rational allocation of the zero vector, only two-phase switching devices are active in each sector, and their switching losses can be reduced by one-third compared with those of traditional PWM strategies. A detailed analysis of the impact of small vectors, which correspond to different zero vectors, on the neutral-point potential is conducted, and a hysteresis control method is proposed to balance the neutral point. This method is simple, does not judge the direction of midpoint currents, and can adjust the switching times of devices and the fluctuation of the neutral-point potential by changing the hysteresis loop width. Simulation and experimental results prove the effectiveness and feasibility of the proposed strategy.

Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene

Wang, Zhen,Yu, Hong-Sheng,Yao, Ru-Yong,Qiu, Wen-Sheng,Yue, Lu,Sui, Ai-Hua,Liu, Xiang-Ping,Liu, Shi-Hai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western-blotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.

Effects of Wind Generation Uncertainty and Volatility on Power System Small Signal Stability

Shi, Li-Bao,Kang, Li,Yao, Liang-Zhong,Qin, Shi-Yao,Wang, Rui-Ming,Zhang, Jin-Ping The Korean Institute of Electrical Engineers 2014 Journal of Electrical Engineering & Technology Vol.9 No.1

This paper discusses the impacts of large scale grid-connected wind farm equipped with permanent magnet synchronous generator (PMSG) on power system small signal stability (SSS) incorporating wind generation uncertainty and volatility. Firstly, a practical simplified PMSG model with rotor-flux-oriented control strategy applied is derived. In modeling PMSG generator side converter, the generator-voltage-oriented control strategy is utilized to implement the decoupled control of active and reactive power output. In modeling PMSG grid side converter, the grid-voltage-oriented control strategy is applied to realize the control of DC link voltage and the reactive power regulation. Based on the Weibull distribution of wind speed, the Monte Carlo simulation technique based is carried out on the IEEE 16-generator-68-bus test system as benchmark to study the impacts of wind generation uncertainty and volatility on small signal stability. Finally, some preliminary conclusions and comments are given.

Comparative Evaluation of Hepatoprotective Activities of Geniposide, Crocins and Crocetin by CCl4-Induced liver Injury in Mice

( Ping Chen ),( Yang Chen ),( Ya Rong Wang ),( Shi Ning Cai ),( Liang Deng ),( Jia Liu ),( Hao Zhang ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Iridoid glycosides (mainly geniposide) and crocetin derivatives (crocins) are the two major active constituents in Gardenia jasminoides Ellis. In the present study, geniposide, crocins, crocin-1 and crocetin were separated from gardenia chromatographically. Then, mice were orally administrated with geniposide (400 mg/kg b.w.), crocins (400 mg/kg b.w.), crocin-1 (400 mg/kg b.w.) and crocetin (140 mg/kg b.w.) once daily for 7 days with CCl4. Hepatoprotective properties were evaluated by biochemical parameters: Administration of geniposide, crocins, crocin-1and crocetin significantly lowered serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) levels in CCl4-treated mice. The reduced glutathione (GSH) levels and antioxidant enzymes (SOD and CAT) activities were also increased by geniposide, crocins, crocin-1 and crocetin. Histopathological examination of livers showed that these components reduced deformability, irregular arrangement and rupture of hepatocyte in CCl4-treated mice. These biochemical results and liver histopathological assessment demonstrated that geniposide, crocetin derivatives and crocetin show comparative beneficial effects on CCl4-induced liver damage via induction of antioxidant defense. Therefore, contents of geniposide and crocetin derivatives should be both considered for hepatoprotective efficacy of Gardenia jasminoides Ellis.

The establishment of IB-SEM numerical method and verification of fluid-solid interaction

Wang, Jing,Li, Shu-cai,Mao, Xuerui,Li, Li-ping,Shi, Shao-shuai,Zhou, Zong-qing Techno-Press 2018 Geomechanics & engineering Vol.15 No.6

The interaction between particles and fluid was investigated by IB-SEM numerical method which is a combination of combing the spectral/hp element method and the rigid immersed boundary method. The accuracy of this numerical method was verified based on the computed results with the traditional body-fitted mesh in numerical simulation of the flow through the cylinder. Then the governing equations of particles motion and contact in fluid are constructed. The movement of the particles and the interaction between the fluid and the particles are investigated. This method avoided the problem of low computational efficiency and error caused by the re-division of the grid when the solids moved. Finally, the movement simulation of multi particles in the fluid was carried out, which can provide a completely new numerical simulation method.