http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Shaohui Ji (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Lifang Jin,Jian Ni,Shaohui Ji,Mei Shen,Jianlong Zhang,Jiwei Han 한국통합생물학회 2014 Animal cells and systems Vol.18 No.4
Mesenchymal stem cells (MSCs) derived from rabbit bone marrow are routinely investigated in cardiovascularand orthopedic models for regenerative medicine application. However, classical medium used for generatingrabbit MSCs (rbMSCs) was supplemented with fetal bovine serum (FBS), which would raise several safetyconcerns and inconsistencies in the generation of MSCs. Here, we tested a serum-free medium for culture ofrbMSCs through the investigation of multiple parameters including cell morphology, expansion, phenotype,and trilineage differentiation. RbMSCs were isolated using the gradient centrifugation method, and expandedin StemPro® MSC SFM in the presence of substrates and basic fibroblast growth factor (bFGF). Cells werethen characterized by phase contrast microscopy observation, cell proliferation analysis, immunocytochemistrystaining, RT-PCR, and qPCR analysis. Similar to that achieved with FBS-containing culture medium, theviability and proliferation of rbMSCs were improved in serum-free medium supplemented with bFGF and precoatedwith CELLstartTM. Serum-free expanded rbMSCs showed the fibroblastic spindle-shape morphology,and were positive for CD29, CD44, and CD73, and negative for CD34, CD45, and CD166. After thecorresponding differentiation, rbMSCs from serum-free condition were demonstrated to differentiate intoadipogenic, osteogenic, and chondrogenic lineages. In addition, differences were also observed betweenrbMSCs cultured with or without serum in terms of their morphology, proliferation, and gene expression levelsof early differentiation transcription factors. In conclusion, this study demonstrates that rbMSCs can beexpanded in a serum-free condition while maintaining the original characteristics of MSCs, which provides auseful tool to understand the basic biological properties of rbMSCs in culture.
-
( Jiaofen Nan ),( Liangliang Zhang ),( Qiqiang Chen ),( Nannan Zong ),( Peiyong Zhang ),( Xing Ji ),( Shaohui Ma ),( Yuchen Zhang ),( Wei Huang ),( Zhongzhou Du ),( Yongquan Xia ),( Ming Zhang ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2018 Journal of Neurogastroenterology and Motility (JNM Vol.24 No.1
Background/Aims The Rome III criteria separated chronic constipation into functional constipation (FC) and constipation-predominant irritable bowel syndrome (IBS-C), but some researchers questioned the partitioning and treated both as distinct parts of a continuum. The study aims to explore the similarity and diversity of brain white matter between FC and IBS-C. Methods The voxel-wise analysis of the diffusion parameters was used to quantify the white matter changes of female brains in 18 FC patients and 20 IBS-C patients compared with a comparison group with 19 healthy controls by tract-based spatial statistics. The correlations between diffusive parameters and clinical symptoms were evaluated using a Pearson’s correlation. Results In comparison to healthy controls, FC patients showed a decrease of fractional anisotropy (FA) and an increase of radial diffusivity (RD) in multiple major fibers encompassing the corpus callosum (CC, P = 0.001 at peak), external capsule (P = 0.002 at peak), corona radiata (CR, P = 0.001 at peak), and superior longitudinal fasciculus (SLF, P = 0.002 at peak). In contrast, IBS-C patients showed FA and RD aberrations in the CC (P = 0.048 at peak). Moreover, the direct comparison between FC and IBS-C showed only RD differences in the CR and SLF. In addition, FA and RD in the CC were significantly associated with abdominal pain for all patients, whereas FA in CR (P = 0.016) and SLF (P = 0.040) were significantly associated with the length of time per attempt and incomplete evacuation separately for FC patients. Conclusion These results may improve our understanding of the pathophysiological mechanisms underlying different types of constipation. (J Neurogastroenterol Motil 2018;24:107-118)
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
