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- 저자 : Seyed Qasem Mousavi (검색결과 2 건)
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Qasem Mousavi Seyed,Reza Bandani Ali,Alaie Ebrahim 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4
The symbiotic microorganisms in the gut of termites play a key role in the digestion of cellulose and nitrogen metabolism. Therefore, disruption of these symbioses activity can open a door toward termite management. Thus, the current study aimed to exploit termite gut bacterial capacity in order to utilize it for efficient termite control. So, in the current study, gut bacteria of Microcerotermes diversus have been extracted, cultivated on both liquid and solid media, and screened with a range of antibiotics to find the most effective antibiotics. Results showed that chloramphenicol and nalidixic acid were the most effective antibiotics in preventing the colony unit formation of the gut bacteria amongst 16 antibiotics tested. Also, from two chitin synthesis inhibitors tested hexaflumuron with an LC 50 of 613.9 µg ml −1 was more toxic than lufenuron with an LC 50 of 1414.5 µg ml −1 . Thus, two antibiotics (chloramphenicol and nalidixic acid) were used simultaneously with a sub-lethal con centration of hexaflumuron against the termite and a variety of factors such as wood consumption rate, running speed, body water content, and tunneling activity were evaluated under laboratory conditions. Results showed that the combination of these two antibiotics (chloramphenicol and nalidixic acid) with hexaflumuron caused a significant decrease in termite consumption rate, running speed, and tunneling behavior, but didn’t affect the body water contents of termites. These results suggest that using a combination of antibiotic/s and hexaflumuron is a promising management practice to get a suitable control measure for the studied termite.
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Ali Darvishzadeh,Ali Reza Bandani,Azam Amiri,Seyed Qasem Mousavi 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.3
The red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is a serious pest of date palms in many Middle East countries. The aimof the current study was to obtain a good understanding of the gut proteolytic activity of R. ferrugineus. Thus, the enzymes in the alimentary tract were isolated and their properties were investigated using a general protease substrates (azocasein and hemoglobin) as well as specific substrates of trypsin, chymotrypsin, elastase, cathepsin L, and cathepsin B. Results showed that in the gut of the RPW both serine and cysteine proteases are present although with different activities. Among the active proteases, trypsin was the most active in the gut followed by chymotrypsin and elastase. The activities of these three proteases in the larval gut were 1.32, 1.12 and 0.32 U, respectively. Among cysteine proteases, cathepsin L and cathepsin B were active in the larval gut. The activity of cathepsin L was 0.82 U while that of cathepsin B was 0.31 U. The order of the activity of the proteases in the larval gut was trypsin N chymotrypsin N cathepsin L N elastase N cathepsin B. The effect of pH on general protease substrates hydrolysis showed two distinct peaks: one in the acidic area (pH 6.0) and another in the alkaline area (pH 10.0); these correspond to cysteine and serine protease activities, respectively. Zymogram analysis along with inhibition studies revealed that four proteases are present in the gut.
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