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Preliminary Experiments on Cloning of Collagenase from C. histolyticum
Han, Song,Seifter, Sam The Official Publication of Korean Academy of Oral 1993 International Journal of Oral Biology Vol.17 No.2
Collagenase from Clostridium histolyticum was purified to homogeneity using several column chromatographic methods in the presence of protease inhibitors. The purified enzyme migrated as one band in SDSPAGE with an apparent Mr of 110,000 and in several test systems, did not exhibit any other proteolytic activity. Automatic Edman degradation of the protein revealed that the primary structure of the aminoterminal region of the protein was IANTNSEKYDFEYLNGL. The protein was cleaved with CNBr and the resulting CNBr peptides were separated by HPLC. A polyclonal antibody against the enzyme was raised and its titer determined on a slot blot using an ABC detection method. DNA from C. histolyticum was prepared, digested partially with Sau3AI and inserted onto a multiple cloning site of the vector plasmid pBluescript SK(-). Host E. coli strain XL1 Blue transformed with the recombinant plasmids was screened with an oligonucleotide probe; several positive clones were thereby revealed.
Sequential Processing: Control of Nanomorphology in Bulk Heterojunction Solar Cells
Wang, Dong Hwan,Moon, Ji Sun,Seifter, Jason,Jo, Jang,Park, Jong Hyeok,Park, O Ok,Heeger, Alan J. American Chemical Society 2011 Nano letters Vol.11 No.8
<P>Bulk heterojunction organic photovoltaic devices based on poly[<I>N</I>-9′′-hepta-decanyl-2,7-carbazole-<I>alt</I>-5,5-(4′,7′-di-2-thienyl-2′,1′,3′-benzothiadiazole) (PCDTBT)/[6,6]-phenyl C<SUB>70</SUB> butyric acid methyl ester (PC<SUB>70</SUB>BM) can be successfully fabricated by a sequential solution deposition process. When the top layer is deposited from an appropriate cosolvent, the PC<SUB>70</SUB>BM penetrates a predeposited bottom layer of PCDTBT during the spin-casting process, resulting in an interdiffused structure with a layer-evolved bulk heterojunction (LE-BHJ) nanomorphology. The PCDTBT:PC<SUB>70</SUB>BM LE-BHJ solar cells prepared with an optimized cosolvent ratio have comparable power conversion efficiency to the conventional BHJ solar cells. The nanomorphology of the optimized PCDTBT:PC<SUB>70</SUB>BM LE-BHJ mixture was found to have better vertical connectivity than the conventional BHJ material.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2011/nalefd.2011.11.issue-8/nl202320r/production/images/medium/nl-2011-02320r_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl202320r'>ACS Electronic Supporting Info</A></P>
Nutritional Management of Kidney Stones (Nephrolithiasis)
( Hae Wook Han ),( Adam M. Segal ),( Julian L. Seifter ),( Johanna T. Dwyer ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.3
The incidence of kidney stones is common in the United States and treatments for them are very costly. This review article provides information about epidemiology, mechanism, diagnosis, and pathophysiology of kidney stone formation, and methods for the evaluation of stone risks for new and follow-up patients. Adequate evaluation and management can prevent recurrence of stones. Kidney stone prevention should be individualized in both its medical and dietary management, keeping in mind the specific risks involved for each type of stones. Recognition of these risk factors and development of long-term management strategies for dealing with them are the most effective ways to prevent recurrence of kidney stones.