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RISS 인기검색어
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- 저자 : Sczekan, Steve (검색결과 1 건)
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Lee, Dong-Hee,Mittag, Maria,Sczekan, Steve,Morse, David,Hastings, J.Woodland 이화여자대학교 생명과학연구소 1993 생명과학연구논문집 Vol.4 No.-
The circadian expressed luciferin-binding protein(LBP)gen from the marine bioluminescent alga Gonyaulax polyedra represents the first dinoflagellate gene that has been cloned and sequenced at both cDNA and genomic levels. Starting with a fragment from the 3'-end of the LBP eDNA that was found by immunoscreening of a cDNA library, genomic clones were obtained by the inverse polymerase chain reaction technique. Full-length cDNA clones were selected by sereening a cDNA library by plaque hybridizations and by polymerase chain reaction amplifications. The LBP sequence has a 2004-nucleotide open reading frame coding for a protein of 668 amino acids(~75kDa). The reading frame and identity of the clone were confirmed by the sequence of an octapeptide obtained from a purified fragment of CNBr-treated LBP. A variant LBP cDNA was found to differ in sequency by ~11% at the DNA level. The untranslated regions of the mRNA are 111 nucleotides (5'-untranslated region) and 158 nucleotides (3'-untranslated region) long, respectively. The LBP gene contains no introns and exhibits certain features not typical for a eukaryotic gene. Its promoter does not include the typical TATA box within ~50 nucleotides upstream of the transcription start site, and the usual poly (A+) signal (AAUAAA) is not present on the end of the LBP mRNA. The copy number of the gene is very high (~1000 copies/cell). However, the universal genetic code and coserved positions relevant for the translational apparatus are maintained.
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