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RISS 인기검색어
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- 저자 : Schwarting, Arthur E. (검색결과 4 건)
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Claviceps paspali 의 액내배양에서의 라이써직산 알카로이드 생성에 대한 비산의 촉진작용
김병각,William J . Kelleher Arthur E . Schwarting ( Byong Kak Kim ) 생화학분자생물학회 1972 BMB Reports Vol.5 No.1
Analysis of the culture filtrates of lysergic acid alkaloid-producing fermentations at intervals during growth of the cultures showed that phosphate is most rapidly depleted from the medium in fermentations that produce the highest levels of alkaloid. In view of the known interference with phosphate metabolism by arsenate, sodium arsenate was added to the culture medium with the intent of creating, in effect, a phosphate deficiency. The addition of arsenate at levels between one-fiftieth and one-twentieth the molar concentration of phosphate resulted in increases in alkaloid production up to 100 per cent. Experiments with ten-times the usual concentration of phosphate showed that the ratio of arsenate to phosphate rather than the absolute concentration of arsenate was most significant in obtaining this effect. Increasing the concentration of phosphate alone (up to twenty times the usual concentration) resulted in substantial increases in alkaloid production. The effect of arsenate on both the rate and the extent of phosphate uptake was determined. Among several inhibitors of electron transport and/or oxidative phosphorylation, only the uncoupler of oxidative phosphorylation (dinitrophenol) was effective in increasing alkaloid production.
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김병국,Kim, Byong-Kak,Kelleher, William J.,Schwarting, Arthur E. 생화학분자생물학회 1972 한국생화학회지 Vol.5 No.1
라이써직산 알카로이드를 생성하는 발효에서 맥각균 Claviceps paspali의 성장과정중 경시적으로 발효액을 분석하여 본바, 가장 높은 농도의 알카로이드를 생성하는 발효액에서 인산이 가장 신속히 소모됨을 알았다. 인산대사가 비산에 의해 방해된다는 점에 착안하여 일종의 인산결핍상태를 만들 목적으로 배양액에다 비산나트륨을 첨가하였다. 인산의 0.02 및 0.05 몰 농도에 해당하는 비산을 첨가하였을때 알카로이드 생성이 100% 까지 증가하는 결과를 나타내었다. 폐지의 정상농도의 10배의 인산을 사용한 실험에서, 알카로이드의 생성증가에 중요한 영향을 미치는 것은 비산의 절대농도가 아니라 인산 대 비산의 비 임을 알게 되었다. 인산 농도만을 증가시켰을 때(정상 농도의 20배까지), 알카로이드 생성이 상당히 증가되는 결과를 초래하였다. 맥각균의 인산 섭취의 범위와 속도에 대한 비산의 영향을 측정하였다. 전자 수송 혹은 산화적 인산화 또는 두 가지 모두를 저지하는 수종의 억제제 중에서 다이나이트로훼놀만이 알카로이드 생성을 촉진시키는 데 유효하였다. Analysis of the culture filtrates of lysergic acid alkaloid-producing fermentations at intervals during growth of the cultures showed that phosphate is most rapidly depleted from the medium in fermentations that produce the highest levels of alkaloid. In view of the known interference with phosphate metabolism by arsenate, sodium arsenate was added to the culture medium with the intent of creating, in effect, a phosphate deficiency. The addition of arsenate at levels between one-fiftieth and one-twentieth the molar concentration of phosphate resulted in increases in alkaloid production up to 100 per cent. Experiments with ten-times the usual concentration of phosphate showed that the ratio of arsenate to phosphate rather than the absolute concentration of arsenate was most significant in obtaining this effect. Increasing the concentration of phosphate alone (up to twenty times the usual concentration) resulted in substantial increases in alkaloid production. The effect of arsenate on both the rate and the extent of phosphate uptake was determined. Among several inhibitors of electron transport and/or oxidative phosphorylation, only the un coupler of oxidative phosphorylation (dinitrophenol) was effective in increasing alkaloid production.
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Structure-rheology elucidation of human blood via SPP framework and TEVP modeling
Matthew Armstrong,Jeff Baker,Jesse Trump,Erin Milner,J. Kenneth Wickiser,Kenneth Cameron,Nick Clark,Kaitlyn Schwarting,Thomas Brown,Dorian Bailey,Corey James,Chi Nguyen,Trevor Corrigan 한국유변학회 2021 Korea-Australia rheology journal Vol.33 No.1
Recent work modeling the rheological behavior of human blood indicates that it has all the hallmark features of a complex material, including shear-thinning, viscoelastic behavior, a yield stress, and thixotropy. After decades of modeling steady state blood data, and the development of simple steady state models, like the Casson and Herschel-Bulkley the advancement and evolution of blood modeling to incorporate more thixo-elasto-visco-plastic (TEVP) features to accurately capture transient flow has renewed interest. With recently collected steady state and oscillatory shear flow rheological data from a DHR-3 using human blood, we show modeling efforts with a contemporary thixo-elasto-visco-plastic (TEVP) model. Best fit rheological model parameters are used to determine values for normal, h ealthy blood and corroborate correlations from literature. Series of physical processes (SPP) analysis is incorporated to illustrate how mechanical properties are tied to the transient, evolving microstructure of human blood and physiological parameters. Using LAOS data predictions of the structure parameter, is compared, and correlated with the transient elastic modulus, .
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The host environment regulates the function of CD8+ graft-versus-host-reactive effector cells.
Chakraverty, Ronjon,Flutter, Barry,Fallah-Arani, Farnaz,Eom, Hyeon-Seok,Means, Terry,Andreola, Giovanna,Schwarte, Sebastian,Buchli, Jennifer,Cotter, Pete,Zhao, Guiling,Sykes, Megan Williams Wilkins 2008 JOURNAL OF IMMUNOLOGY Vol.181 No.10
<P>We have examined how the host environment influences the graft-vs-leukemia (GVL) response following transfer of donor T cells to allogeneic chimeras. Donor T cells induce significant GVL when administered in large numbers to established mixed chimeras (MC). However, when using limiting numbers of T cells, we found that late transfer to MC induced less GVL than did early transfer to freshly irradiated allogeneic recipients. Late donor T cell transfer to MC was associated with marked accumulation of anti-host CD8 cells within the spleen, but delayed kinetics of differentiation, reduced expression of effector molecules including IFN-gamma, impaired cytotoxicity, and higher rates of sustained apoptosis. Furthermore, in contrast to the spleen, we observed a significant delay in donor CD8 cell recruitment to the bone marrow, a key location for hematopoietic tumors. Increasing the numbers of T cells transferred to MC led to the enhancement of CTL activity and detectable increases in absolute numbers of IFN-gamma(+) cells without inducing graft-vs-host disease (GVHD). TLR-induced systemic inflammation accelerated differentiation of functional CTL in MC but was associated with severe GVHD. In the absence of inflammation, both recipient T and non-T cell populations impeded the full development of GVHD-inducing effector function. We conclude that per-cell deficits in the function of donor CD8 cells activated in MC may be overcome by transferring larger numbers of T cells without inducing GVHD.</P>
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