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Wan Abd Al Qadr Imad Wan-Mohtar,Safuan Ab Kadir,Sarina Abdul Halim Lim,Zul Ilham,Siti Hajar-Azhari,Nazamid Saari 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.6
In submerged-liquid fermentation, seven keyparameters were assessed using one-factor-at-a-time toobtain the highest GABA yield using an industrial soysauce koji Aspergillus oryzae strain NSK (AOSNSK). AOSNSK generated maximum GABA at 30 C (194 mg/L) and initial pH 5 (231 mg/L), thus was able to utilizesucrose (327 mg/L of GABA) for carbon source. Sucrose at100 g/L, improved GABA production at 646 mg/L. Singlenitrogen sources failed to improve GABA production,however a combination of yeast extract (YE) and glutamicacid (GA) improved GABA at 646.78 mg/L. Carbon-tonitrogenratio (C8:N3) produced the highest cell (24.01 g/L) and GABA at a minimal time of 216 h. The keyparameters of 30 C, initial pH 5, 100 g/L of sucrose,combination YE and GA, and C8:N3 generated the highestGABA (3278.31 mg/L) in a koji fermentation. AOSNSKpromisingly showed for the development of a new GABArichsoy sauce.
Wan Abd Al Qadr Imad Wan-Mohtar,Christina Viegelmann,Anita Klaus,Sarina Abdul Halim Lim 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1
Underutilized mycelium of Ganoderma lucidum BCCM 31549 has been a significant source of a glucan sulfate (GS) possessing therapeutic activities. GS have been evaluated for their antifungaldemelanizing properties and nitrite oxide production from stimulated RAW264.7 macrophages. GS exhibited antifungal activity against Aspergillus niger A60 with a minimum inhibitory concentration of 60 mg/mL and a minimum fungicidal concentration of 100 mg/mL. At 60mg/mL (sublethal) and 30mg/mL (subinhibitory) doses of GS, the mycelium of A. niger A60 was successfully demelanized with a conidiophore head and black pigment reduction. Additionally, GS successfully stimulated RAW264.7 macrophage cells at a concentration of 500 μg/mL to produce 0.45 μM of nitric oxide. The GSstimulated RAW264.7 macrophages were morphologically similar to those treated with lipopolysaccharide. The results highlight a novel bifunctional property of mycelial GS from G. lucidum.