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Expression of Sara2 Human Gene in Erythroid Progenitors
Jardim, Denis Leonardo Fontes,Cunha, Anderson Ferreira Da,Duarte, Adriana Da Silva Santos,Santos, Camila Oresco Dos,Saad, Sara Terezinha Olalla,Costa, Fernando Ferreira Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.3
A human homologue of Sar1, named Sara2, was shown to be preferentially expressed during erythropoiesis in a culture stimulated by EPO. Previous studies, in yeast, have shown that secretion-associated and Ras-related protein (Sar1p) plays an essential role in protein transport from the endoplasmic reticulum to the Golgi apparatus. Here, we report the molecular analysis of Sara2 in erythroid cell culture. A 1250 bp long cDNA, encoding a 198 amino-acid protein very similar to Sar1 proteins from other organisms, was obtained. Furthermore, we also report a functional study of Sara2 with Real-time quantitative PCR analysis, demonstrating that expression of Sara2 mRNA increases during the initial stages of erythroid differentiation with EPO and that a two-fold increase in expression occurs following the addition of hydroxyurea (HU). In K562 cells, Sara2 mRNA was observed to have a constant expression and the addition of HU also up-regulated the expression in these cells. Our results suggest that Sara2 is an important gene in processes involving proliferation and differentiation and could be valuable for understanding the vesicular transport system during erythropoiesis.
Loss of infectivity of Neospora caninum oocysts maintained for a prolonged time
Rosangela Soares UZEDA,Kattyanne De Souza COSTA,Sara Lima SANTOS,Alexandre Moraes PINHEIRO,Maria Angela Ornelas De ALMEID,Milton M. McALLISTER,Luis Fernando Pita GONDIM 대한기생충학열대의학회 2007 The Korean Journal of Parasitology Vol.45 No.4
RESEARCH ON PRODUCTION AND VALORIZATION OF MEAGRE (Argyrosomus regius) IN PORTUGAL
Pedro Pousao-Ferreira,Laura Ribeiro,Ana Candeias-Mendes,Margarida Gamboa,Sara Castanho,Marisa Barata,Hugo Quental-Ferreira,Maria Emilia Cunha,Florbela Soares,Sara Costa,Margarida Saavedra,Margarida Sa 한국수산과학회 양식분과 2015 한국수산과학회 양식분과 학술대회 Vol.2015 No.5