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Saophuong Neung,Xuan Hoa Nguyen,Kyaw Wai Naing,이영상,김길용 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.2
Diamondback moth, Plutella xylostella, is one of themost destructive insect pests of several crops world-wide. Effectsof Paenibacillus elgii HOA73 and its combined application withorganic sulfur pesticide on Plutella xylostella were evaluated. Results showed that M3 medium composition indicated the bestmedium for optimization of bacterial colony growth, hydrolyticenzyme production, and insecticidal activity and was selected forculturing P. elgii HOA73 in further assays. The highest colonygrowth of P. elgii HOA73 was identified at 5 days after inoculation. Extracted crude enzyme and crude insecticidal compound from P. elgii HOA73 cultured in the optimized medium kill the secondinstar larvae of Diamondback moth in 40 and 50% at 220mg mL−1of crude enzyme and 2% crude extract, respectively. Combinedapplication of organic sulfur pesticide with bacterial suspensionsignificantly killed 85% second instar larvae of Diamondbackmoth, when compared to use of single application: bacterialsuspension (65%) and organic sulfur pesticide (38%), suggestingthat P. elgii HOA73 combined with organic sulfur pesticide couldbe used to control P. xylostella.
Neung, Saophuong,Nguyen, Xuan Hoa,Naing, Kyaw Wai,Lee, Young Seong,Kim, Kil Yong The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.2
Diamondback moth, Plutella xylostella, is one of the most destructive insect pests of several crops world-wide. Effects of Paenibacillus elgii HOA73 and its combined application with organic sulfur pesticide on Plutella xylostella were evaluated. Results showed that M3 medium composition indicated the best medium for optimization of bacterial colony growth, hydrolytic enzyme production, and insecticidal activity and was selected for culturing P. elgii HOA73 in further assays. The highest colony growth of P. elgii HOA73 was identified at 5 days after inoculation. Extracted crude enzyme and crude insecticidal compound from P. elgii HOA73 cultured in the optimized medium kill the second instar larvae of Diamondback moth in 40 and 50% at $220mg\;mL^{-1}$ of crude enzyme and 2% crude extract, respectively. Combined application of organic sulfur pesticide with bacterial suspension significantly killed 85% second instar larvae of Diamondback moth, when compared to use of single application: bacterial suspension (65%) and organic sulfur pesticide (38%), suggesting that P. elgii HOA73 combined with organic sulfur pesticide could be used to control P. xylostella
Yong Seong Lee,Saophuong Neung,Yun Suk Park,Kil Yong Kim 한국토양비료학회 2014 한국토양비료학회지 Vol.47 No.4
In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of 2.0 g L<SUP>-1</SUP>, crab shell powder of 2.0 g L<SUP>-1</SUP> and gelatin of 1.0 g L<SUP>-1</SUP>, respectively with the highest chitinase activity (3.34 U mL<SUP>-1</SUP>) and gelatinase activity (14.15 U mL<SUP>-1</SUP>). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.
Lee, Yong Seong,Neung, Saophuong,Park, Yun Suk,Kim, Kil Yong Korean Society of Soil Science and Fertilizer 2014 한국토양비료학회지 Vol.47 No.4
In this paper, fractional factorial screening design (FFSD) and central composition design (CCD) were used to optimize the medium components for producing chitinase and gelatinase by Lysobacter capsici YS1215. Crab shell powder, nutrient broth and gelatin were proved to have significant effects on chitinase and gelatinase activity by FFSD first. An optimal medium was obtained by using a three factor CCD, which consisted of nutrient broth of $2.0gL^{-1}$, crab shell powder of $2.0gL^{-1}$ and gelatin of $1.0gL^{-1}$, respectively with the highest chitinase activity ($3.34UmL^{-1}$) and gelatinase activity ($14.15UmL^{-1}$). This value was 3.76 and 1.11 fold of the chitinase and gelatinase activity, respectively, compared to the lowest productive medium in the design matrix. In investigating potential of these enzymes partially purified from L. capsici YS1215 for biotechnological use, the crude enzymes was found to be inhibition against pathogenic fungal mycelia: Colletotrichum gleosporioides, Phytophthora capsici, and Rhizoctonia solani. In this study, we demonstrated the optimal medium for producing the chitinolytic and gelatinolytic enzymes by the strain YS1215 and the role of their enzymes that may be useful for further development of a biotechnological use and agricultural use for biological control of phytopathogenic fungi.