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      • Development of an advanced electrochemical biosensing platform for <i>E. coli</i> using hybrid metal-organic framework/polyaniline composite

        Gupta, Arushi,Bhardwaj, Sanjeev K.,Sharma, Amit L.,Kim, Ki-Hyun,Deep, Akash Elsevier 2019 Environmental research Vol.171 No.-

        <P><B>Abstract</B></P> <P>Because of numerous merits (e.g., the possibility of their synthesis in 1-D, 2-D, and 3-D forms, large surface-to-volume ratio, and flexible framework functionality), metal-organic frameworks (MOFs) are envisaged as excellent media for the development of biosensors for diverse analytes present in environmental media. The present research work, for the first time, reports the development of a Cu-MOF based electrochemical biosensor for highly sensitive detection of <I>E. coli</I> bacteria. In order to realize an MOF-based electrochemically active platform, Cu<SUB>3</SUB>(BTC)<SUB>2</SUB> (BTC = 1,3,5-benzenetricarboxylic acid) was mixed with polyaniline (PANI). The spectroscopic/morphological characterizations of the resulting composite were established with the aid of FT-IR, UV–visible spectroscopy, X-ray diffraction, electron microscopy, and surface area analysis. The thin films of Cu<SUB>3</SUB>(BTC)<SUB>2</SUB>-PANI, on an indium-tin oxide (ITO) substrate, were bio-interfaced with anti-<I>E. coli</I> antibodies for use as a novel biosensing electrode. Based on the electrochemical impedance spectroscopy (EIS) technique of signal measurement, the above sensor exhibited high sensitivity to detect very low concentrations of <I>E. coli</I> (2cfu/mL) in a short response time (~2 min) and was also selective in the presence of other non-specific bacteria. As a novel highlight of the research, this new MOF/PANI based detection platform for <I>E. coli</I> has shown improved performance than many of the previously reported electrochemical biosensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cu<SUB>3</SUB>(BTC)<SUB>2</SUB>-PANI based electrochemical biosensor developed for <I>E. coli</I> bacteria. </LI> <LI> EIS based quantification offers highly sensitive detection with LOD of 2 cfu/mL. </LI> <LI> Detection is highly specific even in presence of other non-specific bacteria. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

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        Diversity and seasonal fluctuations in dung beetle (Coleoptera) community in Kurukshetra, India

        Nidhi KAKKAR,Sanjeev K. GUPTA 한국곤충학회 2010 Entomological Research Vol.40 No.3

        Five pitfall traps baited with 150–200 g of fresh cattle dung were installed for 24 h at weekly intervals. A total of 991 dung beetles from 11 genera, 31 species and three subfamilies was obtained. The community was dominated by Oniticellus spinipes individuals by 32.3%. Ten species appeared only once during the collection period and species composition and dominance changed throughout the period. The overall pattern we detected in the organization of the dung beetle community is that the species richness, abundance and diversity rise in September and the 2nd week of October. The dung beetle community was found to be affected by season.

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        Electronic, Magnetic and Optical Properties of 2D Metal Nanolayers: A DFT Study

        Prabal Dev Bhuyan,Sanjeev K. Gupta,Deobrat Singh,Yogesh Sonvane,P. N. Gajjar 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.4

        In the recent work, we have investigated the structural, electronic, magnetic and optical properties of graphene-like hexagonalmonolayers and multilayers (up to fi ve layers) of 3 d -transition metals Fe, Co and Ni based on spin-polarized density functionaltheory. Here, we have taken two types of pattern namely AA-stacking and AB-stacking for the calculations. The bindingenergy calculations show that the AA-type confi guration is energetically more stable. The calculated binding energies of Fe,Co and Ni-bilayer monolayer are − 3.24, − 2.53 and − 1.94 eV, respectively. The electronic band structures show metallicbehavior for all the systems and each confi gurations of Fe, Co and Ni-atoms. While, the quantum ballistic conductancesof these metallic systems are found to be higher for pentalayer than other layered systems. The density of states confi rmsthe ferromagnetic behavior of monolayers and multilayers of Fe and Co having negative spin polarizations. We have alsocalculated frequency dependent complex dielectric function, electronic energy loss spectrum and refl ectance spectrum ofmonolayer to pentalayer metallic systems. The ferromagnetic material shows Different permittivity tensor ( ɛ ), which is dueto high spin magnetic moment for n-layered Fe and Co two-dimensional (2D) nanolayers. The theoretical investigation suggeststhat the electronic, magnetic and optical properties of 3 d -transition metal nanolayers off ers great promise for their usein spintronics nanodevices and magneto-optical nanodevices applications.

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