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Jo, Sangmee A.,Ahn, Kyungsook,Kim, Eunkyung,Kim, Hwa-Su,Jo, Inho,Kim, Doh Kwan,Han, Changsoo,Park, Moon Ho S. Karger AG 2008 Dementia and geriatric cognitive disorders Vol.25 No.2
<P><I>Background:</I> β-Site amyloid precursor protein cleaving enzyme (BACE) is a candidate risk factor for Alzheimer’s disease (AD) from its key role in β-amyloid generation. Previous genetic association studies of <I>BACE1</I> gene have yielded conflicting results. This study is an attempt to clarify whether the common SNP in exon 5 of <I>BACE1</I> (rs638405, Val262) is associated with a risk for late-onset AD. <I>Methods:</I> We genotyped a synonymous C/G polymorphism of <I>BACE1</I> located in exon 5 and apolipoprotein E (ApoE) in 248 AD patients and 224 healthy persons. A meta-analysis with pooled data from four Chinese studies and our results was performed. <I>Results:</I> The allele and genotype frequencies of <I>BACE1</I> polymorphism were not significantly different between cases and controls (p > 0.05) in the Korean population. A meta-analysis of previously published Asian populations including Koreans showed evidence of a weak association (p = 0.0555 for genotypes, p = 0.0352 for alleles). However, a significant association between the CC genotype and AD was observed in the ApoE-ε4-positive groups (p = 0.0044, OR = 1.995; 95% CI = 1.319-3.018). <I>Conclusion:</I> These data suggest that BACE1 polymorphism in exon 5 influences risk for late-onset AD in those carrying the ApoE ε4 allele.</P><P>Copyright © 2008 S. Karger AG, Basel</P>
Han, Changsu,Jo, Sangmee Ahn,Seo, Ji A,Kim, Byoung Gwon,Kim, Nan Hee,Jo, Inho,Park, Moon Ho,Park, Kun Woo Elsevier 2009 ARCHIVES OF GERONTOLOGY AND GERIATRICS Vol.49 No.2
<P><B>Abstract</B></P> <P>Obesity has a strong association with cardiovascular and metabolic diseases, which have also been linked with dementia. While recent studies have reported an association between mid-life obesity and dementia, the role that later-life obesity may have is less clear. A total of 721 community-dwelling elderly (60–85 years old) were selected. Obesity parameters, like body mass index (BMI), waist-hip ratio (WHR), waist circumference (WC), and percent body fat (PBF), as well as cognitive functions were measured over a period of approximately 2 years, and then the relationships between these variables were assessed. The change in cognitive function in the elderly was associated with the baseline assessment of BMI (linearly, <I>β</I> =0.092), WC (quadratic, <I>β</I> =1.333), and PBF (linearly, <I>β</I> =0.097). Using multiple regression analyses, the differences exist in the change of cognitive function over time according to the sex. For men, increased obesity over time when obese in the baseline assessment (BMI, WHR, WC) were associated with a positive change in cognitive function. For women, a decreased obesity over time when obese in the baseline assessment (WHR) and an increased obesity over time when they had a normal adiposity in the baseline assessment (WC) were both associated with cognitive decline. The relationship between obesity and cognitive decline in the elderly is complex and some differences exist between the sexes. The application of the “Jolly Fat” hypothesis to cognitive function can only be applied to elderly men and not to elderly women.</P>
Yoo, Chulbae,Ahn, Kyungsook,Park, Jeong Eun,Kim, Min-Ju,Jo, Sangmee Ahn Elsevier 2010 FEBS letters Vol.584 No.19
<P><B>Abstract</B></P><P>The accumulation of beta amyloid (Aβ) has been a primary target for Alzheimer disease therapeutic strategies. Previously, we discovered an activity from <I>Streptomyces</I> sp. KK565 growth media that inhibits Aβ aggregation. The active component was an aminopeptidase and named <I>Streptomyces</I> sp. KK565 aminopeptidase (SKAP). SKAP cleaved N-terminal amino-acids of Aβ<SUB>1–42</SUB> monomer, inhibited formation of fibrils and protected Aβ<SUB>1–42</SUB>-induced neurotoxicity. Over-expression of a human homolog of SKAP, glutamate carboxypeptidase II (hGCPII) in Aβ-oversynthesizing cells dramatically reduced the Aβ levels. These findings suggest a possible role of M28 family peptidases in preventing Aβ deposits in mammalian brain.</P><P><B>Structured summary</B></P><P>MINT-7992796: <I>SKAP</I> (uniprotkb:Q306T3) <I>physically interacts</I> (MI:0915) with <I>Abeta</I> (uniprotkb:P05067) by <I>protease assay</I> (MI:0435)</P><P>MINT-7992752, MINT-7992778: <I>SKAP</I> (uniprotkb:Q306T3) <I>binds</I> (MI:0407) to <I>Abeta</I> (uniprotkb:P05067) by <I>protease assay</I> (MI:0435)</P>
Awareness of putative risk factors for Alzheimer’s disease among elderly Koreans
Park, Moon Ho,Jo, Sangmee Ahn,Jo, Inho,Kim, Eunkyung,Woo, Eun Kyung,Kim, Sung-Soo,Eun, Su-Yong,Han, Changsu,Park, Min Kyu Blackwell Publishing Ltd 2008 Acta neuropsychiatrica Vol.20 No.1
<P>Objective</P><P>Although there are rapidly growing concerns about the high rates of cognitive dysfunction in Korea, the knowledge of risk factors for Alzheimer’s disease (AD) among the general public in Korea remains to be elucidated.</P><P>Methods</P><P>A total of 2767 randomly selected subjects from the Ansan Geriatric Study were questioned on their knowledge of putative risk factors for AD. Their answers were compared with their sociodemographic data and other variables.</P><P>Results</P><P>The most common stated risk factor was being older (59.6%), followed by head trauma (33.6%) and cerebrovascular disease (30.4%). However, a substandard education, which is a known risk factor, was considered significant by only 9.5% of the subjects. Predictors for a worse knowledge of the risk factors for AD were being older, a lower level of education, lower economic status and the attitude that dementia is not curable.</P><P>Conclusion</P><P>This study revealed that misunderstanding about AD is more prevalent in older subjects and those with a lower level of education, and so public health education on the basic concepts of AD should be targeted at this population.</P>
Song Ji-Hye,Oh Se-Young,Jo Sangmee Ahn 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5
BACKGROUND: Mesenchymal stem cells (MSCs) are considered a potential tool for regenerating damaged tissues due to their great multipotency into various cell types. Here, we attempted to find the appropriate conditions for neuronal differentiation of tonsil-derived MSCs (TMSCs) and expand the potential application of TMSCs for treating neurological diseases. METHODS: The TMSCs were differentiated in DMEM/F-12 (Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12) supplemented with various neurotrophic factors for 7–28 days to determine the optimal neuronal differentiation condition for the TMSCs. The morphologies as well as the levels of the neural markers and neurotransmitters were assessed to determine neuronal differentiation potentials and the neuronal lineages of the differentiated TMSCs. RESULTS: Our initial study demonstrated that DMEM/F12 supplemented with 50 ng/mL basic fibroblast growth factor with 10 lM forskolin was the optimal condition for neuronal differentiation for the TMSCs. TMSCs had higher protein expression of neuronal markers, including neuron-specific enolase (NSE), GAP43, postsynaptic density protein 95 (PSD95), and synaptosomal-associated protein of 25 kDa (SNAP25) compared to the undifferentiated TMSCs. Immunofluorescence staining also validated the increased mature neuron markers, NeuN and synaptophysin, in the differentiated TMSCs. The expression of glial fibrillar acidic protein and ionized calcium-binding adaptor molecule 1 the markers of astrocytes and microglia, were also slightly increased. Additionally, the differentiated TMSCs released a significantly higher level of acetylcholine, the cholinergic neurotransmitter, as analyzed by the liquid chromatographytandem mass spectrometry and showed an enhanced choline acetyltransferase immunoreactivity compared to the undifferentiated cells. CONCLUSION: Our study suggests that the optimized condition favors the TMSCs to differentiate into cholinergic neuron-like phenotype, which could be used as a possible therapeutic tool in treating certain neurological disorders such as Alzheimer’s disease.
Cho, Du-Hyong,Choi, Yoon Jung,Jo, Sangmee Ahn,Ryou, Jungsang,Kim, Jin Yi,Chung, Jongkyeong,Jo, Inho American Physiological Society 2006 American journal of physiology. Cell physiology Vol.291 No.2
<P>Thiazolidinediones (TZDs), synthetic peroxisome proliferator-activated receptor gamma (PPARgamma) ligands, have been implicated in the inhibition of protein synthesis in a variety of cells, but the underlying mechanisms remain obscure. We report that troglitazone, the first TZD drug, acutely inhibited protein synthesis by decreasing p70 S6 kinase (p70S6K) activity in bovine aortic endothelial cells (BAEC). This inhibition was not accompanied by decreased phosphorylation status or in vitro kinase activity of mammalian target of rapamycin (mTOR). Furthermore, cotreatment with rapamycin, a specific mTOR inhibitor, and troglitazone additively inhibited both p70S6K activity and protein synthesis, suggesting that the inhibitory effects of troglitazone are not mediated by mTOR. Overexpression of the wild-type p70S6K gene significantly reversed the troglitazone-induced inhibition of protein synthesis, indicating an important role of p70S6K. Okadaic acid, a protein phosphatase 2A (PP2A) inhibitor, partially reversed the troglitazone-induced inhibition of p70S6K activity and protein synthesis. Although troglitazone did not alter total cellular PP2A activity, it increased the physical association between p70S6K and PP2A, suggesting an underlying molecular mechanism. GW9662, a PPARgamma antagonist, did not alter any of the observed inhibitory effects. Finally, we also found that the mTOR-independent inhibitory mechanism of troglitazone holds for the TZDs ciglitazone, pioglitazone, and rosiglitazone, in BAEC and other types of endothelial cells tested. In conclusion, our data demonstrate for the first time that troglitazone (and perhaps other TZDs) acutely decreases p70S6K activity through a PP2A-dependent mechanism that is independent of mTOR and PPARgamma, leading to the inhibition of protein synthesis in endothelial cells.</P>
정현강(Hyun Gang Jung),안상미(Sangmee Ahn),박문호(Moon Ho Park),김난희(Nan Hee Kim),박건우(Kun Woo Park),조숙행(Sook Haeng Joe),정인과(In Kwa Jung),조인호(Inho Jo),한창수(Changsu Han) 대한노인정신의학회 2006 노인정신의학 Vol.10 No.2
Background : The number and proportion of live-alone elders in Korea have been increasing dramatically. We tried to identify the physical health status of live-alone elders in community and their needs for public health service in order to provide basic data for effective public health service to promote health and quality of life. Methods : The subjects for this study were 38 nurses who provide visit-nursing service to live-alone elders in Seoul and Kyunggi Province. Data were collected by semi-structured questionnaires. Results : The half of live-alone elders had disease, but only half of them took appropriate treatment. Barrier to treatment were difficulty to access to medical center, immobility, lack of drive and also poor insight. Conclusion : To solve the problems related to the elderly living alone in community, the instillation of public health policy that encompass professional medical service and comprehensive team approach are needed.