http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs
Han, Sangjo,Kim, Dongsup BioMed Central 2006 BMC bioinformatics Vol.7 No.-
<P><B>Background</B></P><P>Primer design is a critical step in all types of RT-PCR methods to ensure specificity and efficiency of a target amplicon. However, most traditional primer design programs suggest primers on a single template of limited genetic complexity. To provide researchers with a sufficient number of pre-designed specific RT-PCR primer pairs for whole genes in Arabidopsis, we aimed to construct a genome-wide primer-pair database.</P><P><B>Description</B></P><P>We considered the homogeneous physical and chemical properties of each primer (homogeneity) of a gene, non-specific binding against all other known genes (specificity), and other possible amplicons from its corresponding genomic DNA or similar cDNAs (additional information). Then, we evaluated the reliability of our database with selected primer pairs from 15 genes using conventional and real time RT-PCR.</P><P><B>Conclusion</B></P><P>Approximately 97% of 28,952 genes investigated were finally registered in <I>AtRTPrimer</I>. Unlike other freely available primer databases for <I>Arabidopsis thaliana</I>, <I>AtRTPrimer </I>provides a large number of reliable primer pairs for each gene so that researchers can perform various types of RT-PCR experiments for their specific needs. Furthermore, by experimentally evaluating our database, we made sure that our database provides good starting primer pairs for Arabidopsis researchers to perform various types of RT-PCR experiments.</P>
Fold recognition by combining profile–profile alignment and support vector machine
Han, Sangjo,Lee, Byung-chul,Yu, Seung Taek,Jeong, Chan-seok,Lee, Soyoung,Kim, Dongsup Oxford University Press 2005 Bioinformatics Vol.21 No.11
<P><B>Motivation:</B> Currently, the most accurate fold-recognition method is to perform profile–profile alignments and estimate the statistical significances of those alignments by calculating <I>Z</I>-score or <I>E</I>-value. Although this scheme is reliable in recognizing relatively close homologs related at the family level, it has difficulty in finding the remote homologs that are related at the superfamily or fold level.</P><P><B>Results:</B> In this paper, we present an alternative method to estimate the significance of the alignments. The alignment between a query protein and a template of length <I>n</I> in the fold library is transformed into a feature vector of length <I>n</I> + 1, which is then evaluated by support vector machine (SVM). The output from SVM is converted to a posterior probability that a query sequence is related to a template, given SVM output. Results show that a new method shows significantly better performance than PSI-BLAST and profile–profile alignment with <I>Z</I>-score scheme. While PSI-BLAST and <I>Z</I>-score scheme detect 16 and 20% of superfamily-related proteins, respectively, at 90% specificity, a new method detects 46% of these proteins, resulting in more than 2-fold increase in sensitivity. More significantly, at the fold level, a new method can detect 14% of remotely related proteins at 90% specificity, a remarkable result considering the fact that the other methods can detect almost none at the same level of specificity.</P><P><B>Contact:</B> kds@kaist.ac.kr</P>
한상조(Sangjo Han),최범석(Bum Seog Choi),박무룡(Moo Ryong Park) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11
The paper discusses the development of hydrogen liquefaction turbine. Hydrogen gas has good energy density by weight, but has poor energy density compared with hydrocarbons. To get same energy with hydrocarbon, hydrogen needs large storage tanks. Liquefaction of hydrogen can solve this problem. In Claude cycle, the liquefaction system requires a turbo-expander which is not developed domestically. This research covers the process of developing the hydrogen liquefaction turbo-expander from meridional designs to 3D CFD. Off-design performance is checked in different rotating speed and expansion ratio. To check the performance of the turbo-expander, 3D fully CFD simulation is conducted to check the flow and thermal performance using ANSYS CFX. Due to high expansion ratio(5) required for a hydrogen liquefaction system, multi-stage design is employed in this study.
한상조(Sangjo Han),서정민(JeongMin Seo),최범석(Bum Seog Choi),박준영(Jun-Young Park) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
Recently renewable energy gets a lot of attention to generate electricity. One of them is Ocean Thermal Energy Conversion (OTEC). OTEC system provides the high pressure working fluid to the turbine-generator and produce electricity. However, small temperature difference in seawater requires the turbine design with high efficiency. The paper presents the details of 20kW turbine development for CC-OTEC including the thermal cycle analysis and meridional design of the 20kW turbine for CC-OTEC.
한상조(Sangjo Han),서종범(JongBeom Seo) 대한기계학회 2014 大韓機械學會論文集B Vol.38 No.12
최근 전세계적으로 재생에너지의 관심이 증가하고 있다. 그 중 많은 관심을 받고 있는 것이 상대적으로 낮은 온도의 폐열을 사용하는 유기랭킨사이클(ORC)이다. 유기랭킨사이클은 기존의 증기터빈사이클과 유사한 기술을 사용하지만 낮은 온도의 폐열을 사용하기 위해서 증기대신 냉매를 작동유체로 사용한다. 작동유체를 냉매로 사용함으로 인해 이상기체 가정을 사용할 수 없고, 이는 ORC 시스템에 이용되는 터빈의 설계를 좀 더 복잡하게 만든다. 또, 냉매의 큰 분자량과 복잡한 분자구조로 인하여 낮은 음속을 가지게 되고 이로 인해서 쉽게 초킹 조건에 접근하게 된다. 본 연구에서 R245fa 를 작동유체로 하여 입구온도 124℃에 팽창비 9 의 조건에서 터빈의 효율을 증가시키기 위해 다단으로 설계된 아음속 ORC 터빈을 설계하는 과정과 터빈의 성능에 대하여 설명하고자 한다. 설계된 터빈은 200kW 급의 ORC 발전 시스템에 사용될 예정이다. Recently, there has been a growing interest in sustainable energy. One method that has been used is an organic Rankine cycle using conventional turbine technology with a low-temperature waste heat source. A 200-kW organic Rankine cycle (ORC) system was designed for a waste heat recovery application using R245fa as the working fluid. A radial turbine running at 15,000 rpm was employed to generate more than 200 kW with an expansion ratio of nine. Because an ORC turbine uses a refrigerant as the working fluid, the ideal gas law was not employed to design the turbine. In addition, the complexity of the molecular structure of R245fa made it difficult to design the turbine. Because R245fa has an Ma value of one at a low velocity for the working fluid (about 1/3 of the speed of sound in air) at about 100°C, it easily reaches a supersonic flow condition with a small pressure expansion. To increase the efficiency of the turbine, a dual stage radial-type turbine with a subsonic speed was suggested. This paper will describe the design procedure and performance evaluation of the ORC turbine using R245fa.