http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, Sungchul,Lee, Sanghyun,Shin, Jinwook,Kim, Youngkyun,Evnouchidou, Irini,Kim, Donghyun,Kim, Young-Kook,Kim, Young-Eui,Ahn, Jin-Hyun,Riddell, Stanley R,Stratikos, Efstratios,Kim, V Narry,Ahn, Kwangs Nature Publishing Group, a division of Macmillan P 2011 NATURE IMMUNOLOGY Vol.12 No.10
Major histocompatibility complex (MHC) class I molecules present peptides on the cell surface to CD8<SUP>+</SUP> T cells, which is critical for the killing of virus-infected or transformed cells. Precursors of MHC class I??presented peptides are trimmed to mature epitopes by the aminopeptidase ERAP1. The US2??US11 genomic region of human cytomegalovirus (HCMV) is dispensable for viral replication and encodes three microRNAs (miRNAs). We show here that HCMV miR-US4-1 specifically downregulated ERAP1 expression during viral infection. Accordingly, the trimming of HCMV-derived peptides was inhibited, which led to less susceptibility of infected cells to HCMV-specific cytotoxic T lymphocytes (CTLs). Our findings identify a previously unknown viral miRNA??based CTL-evasion mechanism that targets a key step in the MHC class I antigen-processing pathway.
Kim, Youngjo,Kim, Hyung-Jun,Cha, Sun Joo,Choi, Hyun-Jun,Kim, Hayoung,Lee, Sanghyun,Jeon, Yu-Mi,Lee, Mihye,Lee, Seongsoo,Kim, Kiyoung Elsevier 2019 NEUROBIOLOGY OF AGING Vol.73 No.-
<P><B>Abstract</B></P> <P>Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disorder that is characterized pathologically by the loss of motor neurons. Mutations in the <I>TAF15</I> gene have been implicated in the pathogenesis of ALS. TATA-binding protein associated factor 15 (TAF15) accumulates as cytoplasmic aggregates in neuronal cells, the clearance of which may be a therapeutic strategy for ALS. However, the identification of a novel regulator for protection against a TAF15-induced proteinopathy and the exact pathogenic mechanism of TAF15-induced neurodegeneration remain to be elucidated. Here, we show that parkin directly binds to TAF15 and that parkin overexpression can suppress the defective phenotypes, including the life span and locomotive activity of a TAF15-induced proteinopathy. We also found that overexpression of parkin in neuronal cells leads to a reduction in TAF15 levels, because of the E3 ubiquitin ligase activity of parkin. Our study provides in vivo evidence supporting the use of parkin for neuroprotection in a TAF15-induced proteinopathy and offers new insights into the pathogenic mechanisms underlying TAF15-induced ALS.</P>
Evaluation of Korean wheat cultivars for type I and II resistance to Fusarium head blight in 2013
Sanghyun Shin,Kyeong-Hoon Kim,Chon-Sik Kang,Jae-Han Son,Hag-Sin Kim,In-Duck Choi,Young-Jin Kim,Jong-Chul Park,Kyong-Ho Kim,Jong-Nae Hyun,Chul Soo Park,Kwang-Geun Park 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
Fusarium head blight (FHB), caused by Fusarium graminearum is a major disease problem on wheat and barley in Korea. The objectives of this study were to evaluation of korean wheat cultivars for Type I and Type II resistance to FHB. We screened for Type II resistance in the greenhouse using single floret inoculation and for Type I resistance in the field using spray inoculation. Sumai 3 was used the FHB resistant check. Thirty-two korean wheat cultivars were evaluated for resistance to spread of symptoms within spike. The 2013 field screening with wheat cultivar was located in Kimjae-si Joeonbuk Korea. All plots were inoculated twice. Mist-irrigation was applied to facilitate FHB development. FHB severity was assessed visually 21 days after inoculation on 20 arbitrarily selected spikes per plot. FHB severity was determined as the percentage of symptomatic spikelets from the total of all spikelets. For FHB resistance, the average of FHB severity of Type I exhibited ranging from 21.9% to 77.2% and FHB severity of Type II ranging from 20.8% to 100%. Namhae, Milseong, Geuru, Joen, Anbaek and Sukang were the moderately resistant cultivars while Gobun, Alchan, Dajoong, Eunpa, Shinmichal1, Eunpa and Uri were the most susceptible cultivars for Type II resistance.
Natural occurrence of Fusarium mycotoxins in Korean 32 wheat cultivars harvested in 2011 and 2012
Sanghyun Shin,Kyeong-Hoon Kim,Chon-Sik Kang,Jae-Han Son,Hag-Sin Kim,In-Duck Choi,Young-Jin Kim,Jong-Chul Park,Young-Keun Cheong,Choon-Ki Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Fusarium head blight (FHB) is a major disease problem on wheat and barley around the world. F. graminearum produces trichothecenes mycotoxins such as nivalenol (NIV), deoxynivalenol (DON), and zearalenone (ZEA). The objectives of this study were to survey the natural occurrence of FHB and mycotoxins of 32 Korean wheat cultivars grown in 2011-2012 seasons at the National institute of crop science, Iksan, Korea. There was great deal of rainfall and high humidity during flowering time in May 2011. FHB incidence was counted by Fusarium infected spikes per square meter. The samples of 32 wheat cultivar were collected. The grain and flour samples were to analysis for DON and NIV by gas chromatography and ZEA by high performance liquid chromatography. The result showed that the average of FHB incidence(%) per square meter in 2011 and 2012 were 4.2%, 0.5% respectively. There were significant cultivar differences for FHB incidence ranged from 0% to 24% in 2011. All of 32 wheat cultivars contained 9-2088 ng/g for NIV and ten wheat cultivars contained 5.7-8.5 ng/g for ZEA. In addition, DON concentration of Tapdong, Shinmichal1, and Hanbaek were 217, 35 and 683 ng/g respectively. However, the grain and flour sample harvested in 2012 showed that lower FHB incidence and NIV concentration. These results showed that the 32 wheat cultivars harvested in 2011 were heavily contaminated with Fusarium mycotoxins (NIV, DON, ZEA).
Kim, Yong-Kyoung,Kim, Jae Kwang,Kim, Yeon Bok,Lee, Sanghyun,Kim, Soo-Un,Park, Sang Un American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.8
<P>3-Hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) catalyzes the rate-limiting step in the mevalonate pathway. To elucidate the functions of HMGR in triterpene biosynthesis, Platycodon grandiflorum was transformed with a construct expressing Panax ginseng HMGR (PgHMGR). We used PCR analysis to select transformed hairy root lines and selected six lines for further investigation. Quantitative real-time PCR showed higher expression levels of HMGR and total platycoside levels (1.5–2.5-fold increase) in transgenic lines than in controls. Phytosterols levels were also 1.1–1.6-fold higher in transgenic lines than in controls. Among these lines, line T7 produced the highest level of total platycosides (1.60 ± 0.2 mg g<SUP>–1</SUP> dry weight) and α-spinasterol (1.78 ± 0.16 mg g<SUP>–1</SUP> dry weight). These results suggest that metabolic engineering of P. grandiflorum by Agrobacterium-mediated genetic transformation may enhance production of phytosterols and triterpenoids.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-8/jf304911t/production/images/medium/jf-2012-04911t_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf304911t'>ACS Electronic Supporting Info</A></P>
Kim Kangjin,Lee Sanghun,Park Sang-Chul,Kim Nam-Eun,Shin Chol,Lee Seung Ku,Jung Youngae,Yoon Dankyu,Kim Hyeonjeong,Kim Sanghyun,Hwang Geum-Sook,Won Sungho 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Recent investigations have revealed that the human microbiome plays an essential role in the occurrence of type 2 diabetes (T2D). However, despite the importance of understanding the involvement of the microbiota throughout the body in T2D, most studies have focused specifically on the intestinal microbiota. Extracellular vesicles (EVs) have been recently found to provide important evidence regarding the mechanisms of T2D pathogenesis, as they act as key messengers between intestinal microorganisms and the host. Herein, we explored microorganisms potentially associated with T2D by tracking changes in microbiota-derived EVs from patient urine samples collected three times over four years. Mendelian randomization analysis was conducted to evaluate the causal relationships among microbial organisms, metabolites, and clinical measurements to provide a comprehensive view of how microbiota can influence T2D. We also analyzed EV-derived metagenomic (N = 393), clinical (N = 5032), genomic (N = 8842), and metabolite (N = 574) data from a prospective longitudinal Korean community-based cohort. Our data revealed that GU174097_g, an unclassified Lachnospiraceae, was associated with T2D (β = −189.13; p = 0.00006), and it was associated with the ketone bodies acetoacetate and 3-hydroxybutyrate (r = −0.0938 and −0.0829, respectively; p = 0.0022 and 0.0069, respectively). Furthermore, a causal relationship was identified between acetoacetate and HbA1c levels (β = 0.0002; p = 0.0154). GU174097_g reduced ketone body levels, thus decreasing HbA1c levels and the risk of T2D. Taken together, our findings indicate that GU174097_g may lower the risk of T2D by reducing ketone body levels.
Smart Sensor-based Interface Controlin the Mobile CloudEnvironment
Sanghyun Park,Ilmin Kim,Jinsul kim 보안공학연구지원센터 2014 International Journal of Control and Automation Vol.7 No.10
In this paper we propose the use of cloud services to delivery next-generation dynamic mobile interface. The improvement is focused on existing soft keyboard application. Through a simple sign-in process, the customized keyboard interfaced defined by user own can by synced, stored on the cloud and ready to be apply on other compatible devices of the same user in anytime. With cloud based interface, through specific username and password, various smart mobile devices are able to receive their custom interface. Also, we use Gyro Sensor and Wi-Fi direct to help the users freely share their layout through the internet.