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- 저자 : Saijie Gong (검색결과 2 건)
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Zhaojun Hao,Ding Liu,Saijie Gong,Daqiu Zhao,Jun Tao 한국유전학회 2017 Genes & Genomics Vol.39 No.4
Herbaceous peony (Paeonia lactiflora Pall.) is an ornamental flower with wide applications for landscape. However, its application potential is greatly restricted because it is prone to heat stress injury. Although there are some studies about thermo-tolerance of P. lactiflora which focused on physiological and transcriptional level, the information on post-transcriptional level is lacked. Here, we chose a thermo-tolerant cultivar ‘Zifengyu’ and a moderately thermo-tolerant cultivar ‘Hongyanzhenghui’ under the natural long summer heat to investigate heat responsive miRNAs by miRNA sequencing. A total of 24,008,974 and 21,981,360 raw reads were generated from ‘Zifengyu’ and ‘Hongyanzhenghui’, respectively, as well as 45,016,796 total sRNAs and 6,297,111 unique sRNAs were obtained. Then 271 conserved and 9 novel miRNAs were identified in ‘Zifengyu’, while 236 conserved and 11 novel miRNAs were identified in ‘Hongyanzhenghui’. Among them, 71 known miRNAs with 272 potential target genes and 3 novel miRNAs with 4 potential target genes were significantly differentially expressed. Expression analysis of the 7 candidate miRNAs previously reported to be regulated by heat stress and their target genes were performed. It showed that miR172c-3p, miR395a, miR397a, miR408-5p and miR827 were up-regulated by heat stress, and expressed much higher in thermo-tolerant cultivar ‘Zifengyu’, suggesting that they might be involved in heat stress response at the post-transcriptional level. These results could provide a better understanding about the thermos-tolerant mechanism of P. lactiflora on post-transcriptional level.
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Jun Tao,Zhaojun Hao,Mengran Wei,Saijie Gong,Daqiu Zhao 한국유전학회 2016 Genes & Genomics Vol.38 No.12
Herbaceous peony (Paeonia lactiflora Pall.) is easily injured by heat stress (HS), which greatly restricts its application and promotion. In this study, the thermo-tolerance of three representative P. lactiflora cultivars had been firstly assessed. ‘Zifengyu’ was identified as the thermo-tolerant cultivar with relatively lower values and smaller variations in malondialdehyde, hydrogen peroxide (H2O2) and proline contents under HS. Subsequently, their transcriptomes were sequenced by RNA sequencing (RNAseq) technology to construct a complete database. 81,599 unigenes were obtained, and 34,940 unigenes had been annotated. Moreover, through digital gene expression analysis of thermo-tolerant ‘Zifengyu’ and moderately thermo-tolerant ‘Hongyanzhenghui’, 161 heat stress response genes had been screened involving heat shock protein genes, plant hormone signal transduction related genes, fatty acid synthesis genes, reactive oxygen speciesscavenging genes and secondary metabolites related genes. And the effectively and timely response of these genes to HS could endow thermo-tolerance to ‘Zifengyu’. Among these genes, 11 key thermo-tolerant related genes whose expressions were all significantly up-regulated in ‘Zifengyu’ and ‘Hongyanzhenghui’ during development and the former possessed higher levels could be regarded as the candidate genes, including isoprene synthase gene, 2 peroxidase genes, 3-oxoacyl-acyl carrier protein reductase gene (FabG), 3 transcription factor genes (bHLH, NAC and WRKY), HSP20 and 3 HSP70. These results could provide a better understanding of heat stress response in P. lactiflora, and pave for the breeding of thermo-tolerant cultivars.
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