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Jiang, H.,Jang, M.,Sabo-Attwood, T.,Robinson, S.E. Pergamon Press ; Elsevier [distribution] 2016 Atmospheric environment Vol.131 No.-
The oxidative potential of various secondary organic aerosols (SOA) was measured using dithiothreitol (DTT) assay to understand how organic aerosols react with cellular materials. SOA was produced via the photooxidation of four different hydrocarbons (toluene, 1,3,5-trimethylbenzene, isoprene and α-pinene) in the presence of NO<SUB>x</SUB> using a large outdoor photochemical smog chamber. The DTT consumption rate was normalized by the aerosol mass, which is expressed as DTT<SUB>mass</SUB>. Toluene SOA and isoprene SOA yielded higher DTT<SUB>mass</SUB> than 1,3,5-trimethylbenzene SOA or α-pinene SOA. In order to discover the correlation between the molecular structure and oxidative potential, the DTT responses of selected model compounds were also measured. Among them, conjugated aldehydes, quinones, and H<SUB>2</SUB>O<SUB>2</SUB> showed considerable DTT response. To investigate the correlation between DTT response and cell responses in vitro, the expression of biological markers, i.e. IL-6, IL-8, and HMOX-1 were studied using small airway epithelial cells. Higher cellular expression of IL-8 was observed with toluene SOA exposure compared to 1,3,5-trimethylbenzene SOA exposure, which aligned with the results from DTT assay. Our study also suggests that within the urban atmosphere, the contribution of toluene SOA and isoprene SOA to the oxidative potential of ambient SOA will be more significant than that of α-pinene SOA.
Yu, Zechen,Jang, Myoseon,Sabo-Attwood, Tara,Robinson, Sarah E.,Jiang, Huanhuan Elsevier 2017 Toxicology in vitro Vol.42 No.-
<P><B>Abstract</B></P> <P>To better characterize biological responses to atmospheric organic aerosols, the efficient delivery of aerosol to <I>in vitro</I> lung cells is necessary. In this study, chamber generated secondary organic aerosol (SOA) entered the commercialized exposure chamber (CULTEX® Radial Flow System Compact) where it interfaced with an electrostatic precipitator (ESP) (CULTEX® Electrical Deposition Device) and then deposited on a particle collection plate. This plate contained human lung cells (BEAS-2B) that were cultured on a membrane insert to produce an air-liquid interface (ALI). To augment <I>in vitro</I> assessment using the ESP exposure device, the particle dose was predicted for various sampling parameters such as particle size, ESP deposition voltage, and sampling flowrate. The dose model was evaluated against the experimental measured mass of collected airborne particles. The high flowrate used in this study increased aerosol dose but failed to achieve cell stability. For example, RNA in the ALI BEAS-2B cells <I>in vitro</I> was stable at 0.15L/minute but decayed at high flowrates. The ESP device and the resulting model were applied to <I>in vitro</I> studies (i.e., viability and IL-8 expression) of toluene SOA using ALI BEAS-2B cells with a flowrate of 0.15L/minute, and no cellular RNA decay occurred.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A dose model to predict the delivery of particles to ALI cells <I>in vitro</I> using ESP </LI> <LI> Optimal exposure conditions using ESP to obtain the stability of <I>in vitro</I> cells </LI> <LI> Application of the ESP device and the dose model to study <I>in vitro</I> toxicity of SOA </LI> </UL> </P>