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Aniruddha Sarker,Sang-Hyeob Lee,Se-Yeon Kwak,Rakhi Nandi,Jang-Eok Kim 한국농약과학회 2021 한국농약과학회 학술발표대회 논문집 Vol.2021 No.11
3,5-dichloroaniline (3,5-DCA), the most toxic of the mono- and dichloroaniline metabolites of dicarboximide fungicides, has been identified as an emergent environmental pollutant. In this study, the immobilized laccase-mediator-system (ILMS) is used to investigate the biocatalytic detoxification of 3,5-DCA, and catechol is discovered to be the most effective phenolic mediator for increased bioconversion. Laccase loading on microporous support surfaces could be as high as 36.4mg/g. In comparison to free laccase, immobilized laccase on microporous support surfaces had remarkable temperature stability, pH adaptability, storage stability, and reusability (six consecutive reaction cycles). In the presence of catechol, the immobilized laccase efficiently removed investigated 3,5-DCA (99-100%) in the aqueous medium after 72 hours, according to the ILMS assay. Using an ILMS assay, we were able to identify three coupling reaction products during the catalysis of 3,5-DCA in this work. We hypothesized a reaction pathways for the biotransformation of 3,5-DCA by immobilized laccase based on the identified coupling reaction products. This is the first time a pre-field evaluation of biocatalysis utilizing fungal laccase has been done before a real-field investigation. In this pre-field mimic study, laccase immobilization on microporous support surfaces, zeolite, and bentonite was shown to be potentially useful in the sustainable environmental remediation of aniline metabolite (i.e., 3,5-DCA) derived from dicarboximide fungicides.
Aniruddha Sarker,Taek-Kyum Kim,Won-Tae Jeong 한국농약과학회 2023 농약과학회지 Vol.27 No.3
The persistence, mobility and distribution patterns of phorate and terbufos in soil was not well explored. As a result, this study is designed to evaluate the residual uncertainty and leaching potential of phorate and terbufos in packed soil columns. The leaching and distribution behavior of applied pesticides will indicate the soil-pesticide interaction and groundwater contamination through soil column leaching. In this study, a comparative soil column study is conducted following the OECD guidelines for leaching soil column test for exploring the distribution, and mobility of phorate and terbufos including their metabolic transformation into soil column. Briefly, the packed soil column (5 cm diameter, 50 cm height) were filled up to 30 cm, and oversaturated by capillary waters. After leaching test, the leachate and soil sections (each section for 10 cm depth) were analyzed for parent pesticides and transformed metabolites. According to GUS index, both phorate and terbufos considered immobile pesticide (no residue is detected in the leachate) with low to extremely low leachability. Besides, the major pesticides and metabolite concentration (˃90% recovered) was found in the upper layer soil (0-10 cm) followed by subsoils. Among the transformed metabolites, sulfone and sulfoxide were the predominant for both pesticides. The degradation of phorate and terbufos was controlled by soil moisture, organic matter, and soil texture, respectively. In summary, the persistence, distribution and metabolic transformation of phorate and terbufos is limited within the vadose zone soil (0- 10 cm) and no parent pesticides were detected in leachate due to slow leaching potential and immobility.
( Aniruddha Sarker ),( Jang-eok Kim ) 한국환경농학회 2018 한국환경농학회 학술대회집 Vol.2018 No.-
Phosphorus (P) is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Thus P fixation bounds availability of P to plants in tropical soil, which is a major constraint for crop production. Our research was laid out to isolate, screen and characterize phosphate solubilizing bacteria (PSB) from wheat rhizosphere and evaluate their efficacy in Phosphorus nutrition. Upon screening, nine isolated strains showing varying level of phosphate solubilizing activity in both agar plate and broth assays using Pikovskaya’s screening medium. The continual decrease of pH of the culture media was observed with increased bacterial growth suggesting that these bacteria might secrete organic acids to solubilize insoluble phosphorus. In vitro wheat seedling bioassay with two superior PSB stains (PSB1 and PSB8) and varying sources of P revealed that both bacterial strains significantly enhanced seedling growth (shoot and root length, shoot and root dry weight) and nutrient contents (%N, %P and %K) in plant tissue compared to control (no PSB). The performance of PSB8 was higher to PSB1 in respect of all growth and yield contributing parameters studied. The PSB8 was identified as Pseudomonas sp. through 16S rRNA gene sequencing. These findings suggest that Pseudomonas sp. (PSB8) isolated from wheat might be useful for improving P nutrition in wheat in soils with low available Phosphorus.
산화 촉매를 이용한 procymidone 살균제 및 그 대사물 3,5-dichloroaniline 향상된 변형
아니루다사커 ( Aniruddha Sarker ),이상협 ( Sang-hyeob Lee ),곽세연 ( Se-yeon Kwak ),김장억 ( Jang-eok Kim ) 한국환경농학회 2022 한국환경농학회 학술대회집 Vol.2022 No.-
In this study, oxidative catalysts are assessed for sustainable remediation of procymidone and its metabolite 3,5-dichloroaniline (3,5-DCA). The laccase-mediator system (LMS) was employed to transform 3,5-DCA. Amongst the investigated treatments, 2.0 U/mL fungal laccase, and 2.0 mM catechol are the optimum reactant concentrations during oxidative catalysis of 3,5-DCA under acidic reaction conditions (pH 5.0). Besides, heterogeneous catalysis in presence of various types of MnO<sub>2</sub> catalysts is revealed as the rapid oxidative strategy for procymidone and its metabolite. Among the studied MnO<sub>2</sub> catalysts, δ-MnO<sub>2</sub> performed well, when compared to the solitary influence of peroxides and ozone. Further, the reaction mechanism for the accelerated transformation of aniline metabolite by heterogeneous catalysis is explored. However immobilized laccase has exhibited a wide range of pH adaptability (65.35% residual activity at pH 7.0) and thermal stability (56.51% residual activity at 60°C). The reusability (six consecutive reaction cycles) of immobilized laccase and effective removal of 3,5-DCA were the unique feature of the sustainable and cost-effective catalysts through immobilized laccase. The pioneering finding of this study identified and proposed three coupling reaction products during oxidative remediation of investigated 3,5-DCA in presence of a phenolic mediator.