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Geometric Optimization of Micro Drills using Taguchi Methods and Response Surface Methodology
윤해성,Renzhe Wu,이태무,안성훈 한국정밀공학회 2011 International Journal of Precision Engineering and Vol.12 No.5
Micro drill-bits for halogen-free printed circuit boards were analyzed using Taguchi methods and response surface methodology (RSM). Since micro drills have many shape factors, which are not easy to describe exactly, Taguchi methods are one of the most common optimization approaches. However, Taguchi methods can only optimize among fixed variables. Using RSM, one can create a regression surface. Optimal shapes of micro drills were determined using a normalized sum of various measures of performance. It was suggested that RSM combined with other methodologies should be used with more shape factors to further analyze the performance of micro drill-bits.
Ahn, Sung-Hoon,Lee, Kyung-Tae,Kim, Hyung-Jung,Wu, Renzhe,Kim, Ji-Soo,Song, Sung-Hyuk 한국정밀공학회 2012 International Journal of Precision Engineering and Vol.13 No.4
Traditionally, hard or high-stiffness materials are used to achieve morphing but only with limited magnitudes of deformation. Soft and low-stiffness materials are required to implement soft morphing with large deformations. In this paper, a novel concept for soft morphing structures that makes use of smart soft composites (SSC) is introduced. These materials have the following characteristics: 1) Large deformations from smart actuators and soft anisotropic materials embedded in a soft matrix material, 2) Utilization of the coupling effect for in-plane/bending/twisting deformations of the morphing structure to achieve complex morphed shapes, 3) Semi-automated three-dimensional (3D) fabrication via 3D printing processes. Classical lamination theory is used to express in-plane deformation, out-of-plane deformation, and twisting and coupling of these deformations. 3D-printed-scaffold and/or weaving methods can be used to fabricate anisotropic materials for SSCs. An integrated SSC in a cantilever shape is fabricated using SMA (Shape memory alloy)/ABS/PDMS and tested as an example to show large deformation with bend/twist coupling effect.
Expression profiles of microRNAs in skeletal muscle of sheep by deep sequencing
Zhi-Jin Liu,Cun-Yuan Li,Xiao-Yue Li,Yang Yao,Wei Ni,Xiang-Yu Zhang,Yang Cao,Wureli Hazi,Dawei Wang,Renzhe Quan,Shuting Yu,Yuyu Wu,Songmin Niu,Yulong Cui,Yaseen Khan,Shengwei Hu 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.6
Objective: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative real-time polymerase chain reaction. To further investigate the function of miRNAs, candidate targeted genes were enriched for analysis using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment. Results: The results showed total of 1,086 known miRNAs and 40 new candidate miRNAs were detected in prenatal and postnatal skeletal muscle of sheep. In addition, 345 miRNAs (151 up-regulated, 94 down-regulated) were differentially expressed. Moreover, miRanda software was performed to predict targeted genes of miRNAs, resulting in a total of 2,833 predicted targets, especially miR-381 which targeted multiple muscle-related mRNAs. Furthermore, GO and KEGG pathway analysis confirmed that targeted genes of miRNAs were involved in development of skeletal muscles. Conclusion: This study supplements the miRNA database of sheep, which provides valuable information for further study of the biological function of miRNAs in sheep skeletal muscle.