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        Characterization and phylogenetic analysis of the complete mitogenome of a rare cavefish, Sinocyclocheilus multipunctatus (Cypriniformes: Cyprinidae)

        Renyi Zhang,Xue Wang 한국유전학회 2018 Genes & Genomics Vol.40 No.10

        The genus Sinocyclocheilus is a representative group of cave creatures. However, genetic studies on Sinocyclocheilus are rare. The primary objective of this study was to explore the structure and feature of the complete mitochondrial genome of S. multipunctatus, and reconstruct the mitogenomic phylogeny of Sinocyclocheilus. The mitochondrial DNA of S. multipunctatus was amplified by overlapping PCR fragments. The mitogenome was assembled by the SeqMan and annotated using MitoAnnotator. The phylogenetic tree was established using the Bayesian inference and Maximum likelihood methods. The mitogenome of S. multipunctatus is a typical circular molecule of 16,586 bp with base composition A (31.25%), T (25.90%), G (16.35%), and C (26.50%), and consists of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs) genes, two ribosomal RNAs, and a 931 bp control region. Phylogenetic analysis reveals two clades in the Sinocyclocheilus with robust support. S. multipunctatus is close to a newly discovered cavefish, S. ronganensis. We obtained and described the complete mitogenome of S. multipunctatus, and investigated its phylogenetic status, which may provide a valuable resource for future phylogenetic analyses and population genetic studies in Sinocyclocheilus.

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        FAILURE ANALYSIS OF A BRASS SYNCHRONIZER RING IN THE GEARBOX OF VEHICLE

        Gao Hongchen,Xue Song,Renyi Zhang,Peng Yi,Zheng Lixuan 한국자동차공학회 2023 International journal of automotive technology Vol.24 No.2

        Synchronizers are the heart for vehicles. The reliability of synchronizer ring in the gearbox affects the operation of the entire systems of vehicles. In this paper, a pre-failure of synchronizer ring made of brass was investigated. Macro and micro analysis, chemical composition analysis, mechanical property test and finite element method were conducted on the synchronizer ring. The analysis results showed that the content of element in the micro area of the outer ring exceeded the standard, which caused the increases of hardness and brittleness of material. The resisting crack propagation ability of material was reduced due to the element segregation. It was concluded that the synchronizer ring was fractured due to the high hardness and brittleness of material, combined with the stress concentration. Finally, countermeasures were proposed to address similar problems.

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        pH and Redox Dual Stimuli-Responsive Injectable Hydrogels Based on Carboxymethyl Cellulose Derivatives

        Yueqin Shen,Xian Li,Yawen Huang,Guanjun Chang,Ke Cao,Junxiao Yang,Renyi Zhang,Xueying Sheng,Xu Ye 한국고분자학회 2016 Macromolecular Research Vol.24 No.7

        pH and redox dual stimuli-responsive injectable hydrogels were prepared by cross-linking oxidized carboxymethyl cellulose (oxi-CMC) with 3,3'-dithiobis (propionohydrazide) (DTP) via Schiff base reaction under physiological condition. The hydrogels showed good performance such as tunable gelling time, appropriate rheology properties, high swelling ratio and low degradation rate. In vitro release studies confirmed that bovine serum albumin (BSA) as a model drug exhibited a sustainable release at pH 7.4 and an accelerated release under a lower pH 5.0 and/or reducing environments. The results signified that oxi-CMC/DTP hydrogels could be an attractive candidate for drug delivery system, tissue engineering or cell scaffold materials.

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