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RISS 인기검색어
- 검색키워드
- 저자 : Reiza Dolendo Ventura (검색결과 3 건)
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An Overview of Laser-assisted Bioprinting (LAB) in Tissue Engineering Applications
Reiza Dolendo Ventura 대한의학레이저학회 2021 MEDICAL LASERS Vol.10 No.2
Biological tissues and organs are composed of different arrays of cells, biochemical cues, and extracellular matrices arranged in a complex microarchitecture. Laser-Assisted Bioprinting (LAB) is an emerging and promising technology that is reproducible with high accuracy that can be used for fabricating complex bioengineered scaffolds that mimic tissues and organs. The LAB process allows researchers to print intricate structural scaffolds using cells and different biomaterials essential for facilitating cell-scaffold interaction and to induce tissue and organ regeneration which cannot be achieved in a traditional scaffold fabrication. This process can fabricate artificial cell niches or architecture without affecting cellular viability and material integrity. This review tackles the basic principles and key aspects of Laser-Assisted Bioprinting. Recent advances, limitations, and future perspectives are also discussed.
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A review on photobiomodulation therapy for olfactory dysfunction caused by COVID-19
Reiza Dolendo Ventura 대한의학레이저학회 2022 MEDICAL LASERS Vol.11 No.2
Olfactory dysfunction is a loss of smell caused by a number of conditions such as chronic rhinosinusitis, neurodegenerative disease, aging, and viral infection, and it is a common symptom Coronavirus Disease 2019 (COVID-19). Photobiomodulation (PBM) is a non-invasive, safe, and effective treatment for a range of disorders. It has an anti-inflammatory action and stimulates tissue healing, which can help with the reversal of olfactory dysfunction. Due to the limited options for immunocom-promised patients, there is currently a need for an alternative treatment method for olfactory dysfunction. This review highlights the most recent research on the use of PBM for the treatment of olfactory dysfunction.
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Reiza Dolendo Ventura,Jun-Sang Bae,Eun Hee Kim,Shin Hyuk Yoo,Gwanghui Ryu,Ji-Hun Mo 대한의학레이저학회 2022 MEDICAL LASERS Vol.11 No.4
Background and Objectives: The goal of this study was to assess the effects of photobiomodulation (PBM) on the neuronal differentiation of olfactory stem cells (OSCs).Materials and Methods: The OSCs were irradiated with different wavelengths (740 nm and 850 nm) and different energy densities (5 J/cm2 and 10 J/cm2). Immunofluorescence staining and polymerase chain reaction (PCR) analysis were utilized to evaluate the effects of PBM on the OSCs.Results: PCR analyses showed lower neuroepithelial stem cell protein (Nestin) expression in the 740 nm groups (energy densities 5 and 10 J/cm2), and increase in the 850 nm-10 J/cm2 compared to the control. SRY-box transcription factor 2 (Sox2) was downregulated in the 740 nm-10 J/cm2 and elevated in the 850 nm-10 J/cm2 group. Increased glial fibrillary acidic protein (GFAP) expression in the 740 nm groups. Microtubule-associated protein 2 (Map2) expression increased in all groups. Futhermore, Doublecortin (Dcx) expressions increased in 740 nm groups while decreased in 850 nm groups. The expressions of the leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) varied in 740 nm groups and increased in 850 nm groups. Increased expressions in 740 nm groups and decreased expressions in 850 nm groups in cluster of differentiation 36 (CD36). Early B-cell factor 1 (Ebf1) expressions were lower in 740 nm groups and higher in 850 nm groups. Immunofluorescence staining revealed increased tubulin beta 3 (Tubb3) and olfactory marker protein (OMP) intensities in 850 nm-5 J/cm2.Conclusion: The PBM treatment showed positive effects on the maturation and development of OSCs into mature olfactory neurons.
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