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      • Green Synthesis of Non-Purified Silver Nanoparticles Using Morinda citrifolia (Noni) Fruit Extract for Cytotoxicity Assay against MCF-7 (Human Breast Cancer)

        Reisha Claffel Z. Ferraren,Peter Gabriel A. Muaña,Benito A. Baje 국제과학영재학회 2017 APEC Youth Scientist Journal Vol.9 No.1

        With the problem of cancer on the rise, a need to explore alternative cures such as nanoparticles (NPs) exists. The usual production of these NPs is costly and hazardous, which highlights the need for much eco-friendly and cost-efficient techniques. In this study, silver (Ag) NPs were synthesized using green synthesis method from the fruit extract of Morinda citrifolia. Fourier Transform Infrared (FT-IR) Spectroscopy was used to identify the functional groups. Morphological assessment of the synthesized NPs was done using Scanning Electron Microscopy (SEM). Determination of the elemental composition was done using Energy Dispersive X-ray (EDX). The effect of the synthesized NPs on MCF-7 human breast cancer cell line was evaluated using MTT Cytotoxicity Assay. SEM images revealed nanospherical particles with sizes ranging from 28 nm to 136 nm. EDX spectroscopy evaluations suggested the successful formation of NPs with purity of 32.20%. FT-IR showed a number of peaks that represent different functional groups of biological origin. The green synthesized Ag NPs exhibited cytotoxicity against human breast cancer cell (MCF-7) with inhibitory concentration (IC50) greater than 100 μg/mL. Data indicated that the green synthesized NPs possessed anti-cancer activity. No significant differences were revealed among the anticancer activities of the silver nanoparticles and among the anticancer activities of the positive control, Doxorubicin®, in all respective concentrations. The green synthesized Ag NPs were potential alternative cures for cancer.

      • Low-Cost Photoplethysmographic Circuit for Heart Rate Measurement

        Reisha Claffel Z. Ferraren,Lindly Van S. Medrano 국제과학영재학회 2016 APEC Youth Scientist Journal Vol.8 No.1

        Heart rate measurement helps in assessing the condition of the cardiovascular system. Today, there are many other methods to measure heart rates such as Phonocardiogram, ECG, blood pressure wave form, and pulse meters but these methods are clinical and expensive. Because of these, a need exists for cheap sensing devices that can measure pulse in an accurate but much easier way. In this study, a low-cost photoplethysmographic circuit was fabricated with the use ofLM358 Operational Amplifier, photodiode, infrared LED, resistors, and potentiometers. The heartbeats per minute of five volunteers were recorded. Three trials were done per volunteer. Data showed that the average heartbeats per minute for the five volunteers with standard deviations are 67.33 ± 1.53, 63.00 ± 2.00, 65.67 ± 2.52, 70.33 ± 1.53, and 65.33 ± 1.53 respectively. The fabricated circuit successfully measured the heart rates of five people. The experiment established the potential of low-cost do-it-yourself photoplethysmographic circuits as alternatives to the expensive medical heart rate devices currently being used in hospitals and clinics.

      • HUMAN LOCOMOTION VIBRA-E (VIBRATIONAL ENERGY) HARVESTER PROTOTYPE FOR POWERING LIGHT EMITTING DIODE

        Reisha Claffel Z. Ferraren,Peter Gabriel A. Muaña 국제과학영재학회 2015 APEC Youth Scientist Journal Vol.7 No.2

        With the global energy crisis, many technologies in energy harvesting, such as solar, wind, geothermal, and hydraulic power plants, have been developed. This project envisioned to explore the potential of vibrations as a sustainable alternative source of energy through a simple and easy-to-make harvester. Piezoelectric materials that convert vibrations from human activities into energy were applied. A digital multi-meter was used to continually monitor the voltage output of the system. Three trials were done per monitoring. Actual testing to light emitting diode was also performed. Data showed that an output voltage was successfully generated. An average output voltage of 0.52V had a charging time of 20.56 seconds, 1.03V in 33.16 seconds, 1.52V in 60.52 seconds, 2.02V in 88.30 seconds, and 2.34V in 119.71 seconds. An average discharging time of 41.86 seconds per 1mV drop was also recorded. Results suggested that a fully charged device could have a theoretical continuous illumination of approximately 26 hours. In addition, the fabricated device successfully powered a light emitting diode for possible lighting purposes. The experiment established the viability of converting vibrations from human locomotion using a simple wooden box energy harvester and the potential of vibration as one of the most promising sources of energy for powering devices with low voltage electric requirements.

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