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Patel, Sanjay K.S.,Anwar, Muhammad Z.,Kumar, Ashok,Otari, Sachin V.,Pagolu, Ravi T.,Kim, Sang-Yong,Kim, In-Won,Lee, Jung-Kul Elsevier 2018 Biochemical engineering journal Vol.132 No.-
<P><B>Abstract</B></P> <P>The structural morphology and composition of a support play a key role in the performance of nanoparticle-based enzymatic biosensors. In the present study, the influence of different functional groups, including glutaraldehyde, 3-aminopropyltriethoxysilane, carbodiimide, cyano, and polyethyleneimine for the immobilization of laccase on synthesized Fe<SUB>2</SUB>O<SUB>3</SUB> yolk-shell and commercially available Fe<SUB>2</SUB>O<SUB>3</SUB>, SrFe<SUB>12</SUB>O<SUB>19</SUB>, and Y<SUB>3</SUB>Fe<SUB>5</SUB>O<SUB>12</SUB> particles was analyzed. Glutaraldehyde-activated particles showed higher laccase activity after immobilization and higher relative detection currents for 2,6-dimethoxyphenol (2,6-DMP). The multi-shelled structural morphology of Fe<SUB>2</SUB>O<SUB>3</SUB> yolk-shell particles significantly improved the biosensing properties of immobilized laccase compared to that of spherical pure Fe<SUB>2</SUB>O<SUB>3</SUB> and composite SrFe<SUB>12</SUB>O<SUB>19</SUB> and Y<SUB>3</SUB>Fe<SUB>5</SUB>O<SUB>12</SUB> particles. The prepared biosensors showed high selectivity towards 2,6-DMP, with a sensitivity of 452 μA/mM/cm<SUP>2</SUP>. Under optimum conditions, the linear ranges of detection were as follows: 2,6-DMP (0.025–750 μM), guaiacol (0.10–250 μM), pyrogallol (0.25–250 μM), and 3,4-dihydroxy-<SMALL>L</SMALL>-phenylalanine (1.0–125 μM), with limit of detection values of 0.010, 0.052, 0.093, and 0.273 μM, respectively. Laccase immobilized on bio-friendly multi-shelled Fe<SUB>2</SUB>O<SUB>3</SUB> yolk-shell particles showed a broad linear range of detection, the lowest limit of detection, high sensitivity and stability, good reproducibility, anti-interference and recovery, and insignificant inhibition by laccase inhibitors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Fe<SUB>2</SUB>O<SUB>3</SUB> yolk-shell particles were used to prepare laccase biosensors. </LI> <LI> Particle composition and morphology exhibited significant variation in biosensing. </LI> <LI> Fe<SUB>2</SUB>O<SUB>3</SUB> yolk-shell biosensor showed a high selectivity towards 2,6-dimethoxyphenol. </LI> <LI> A broad linear range of detection with the lowest limit of detection (0.01 μM) was observed. </LI> </UL> </P>
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Ratnesh Kumar Patel,Ravi Shankar,Prateek Khare,Prasenjit Mondal 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7
This study demonstrates the optimization of treatment option, an integrated advanced-oxidation process(AOP) based approach where integration of two processes, ultrasonication (US) and electrocoagulation (EC), wereapplied for sugar industry wastewater (SIW) treatment. Experimental results confirm that the individual US and ECprocesses for SIW treatment are found to be inefficient, as only 16% COD removal (equivalent to COD removal:268mg/L) is achieved with US process in 20min, while 68% COD removal (equivalent to COD removal: 1,142mg/L)is achieved in 60 min operation time with EC process. Encouraging results were obtained after integration of US(10.6% COD removal in 20min equivalent to COD removal from 1,680mg/L to 1,502mg/L) with EC (82% CODremoval in 30 min equivalent to COD removal from 1,502mg/L to 270mg/L) process, which is collectively classified asultrasonication-electrochemical (US+EC) process. Overall integration of US and EC process ultimately increased theCOD removal up to ~84% (equivalent to COD removal from 1,680mg/L to 270mg/L) in comparatively shorter operatingtime (US+EC=30min). Calorific value of sludge and scum obtained after EC treatment was determined as 3.69and 2.87MJ/kg, respectively. Treatment cost of sono-electrochemical is estimated based on 1 kg COD removal or 1m3wastewater as 1.40 or 1.974 $, respectively, which is found on lower ends when compared with the many other availabletreatment technologies.
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3D Printing: It’s Microfluidic Functions and Environmental Impacts
Abhishek Sharma,Jitendra K. Pandey,Surajit Mondal,Amit Kumar Mondal,Soumadeep Baksi,Ravi Kumar Patel,추원식 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.4 No.3
Innovative micro products essential for the utilization of a wide variety of macro subjects have complicated three-dimensional (3D) microstructures in addition to a high aspect ratio. Till date, many micro manufacturing processes have been developed, but a specific class of such processes is applicable for fabrication of true 3D micro assembly. The aptitude to process a broad range of materials and the ability to fabricate functional and geometrically complicated, 3D microstructures provides the additive manufacturing (AM) processes which significant profits over traditional methods, such as lithography-based or micromachining approaches investigated widely in the past. In this paper, 3D micro-AM processes have been classified into three main groups, including scalable micro-AM systems, 3D direct writing, and hybrid processes, and the key processes have been reviewed comprehensively. Principle and recent progress of each 3D micro-AM process have been described, and the advantages and disadvantages of each process have low-cost along with its occupational health safety & environmental issues.
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